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- Publisher Website: 10.1016/j.bbrc.2004.02.166
- Scopus: eid_2-s2.0-1642338831
- PMID: 15044100
- WOS: WOS:000220579300022
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Article: Human MCRS2, a cell-cycle-dependent protein, associates with LPTS/PinX1 and reduces the telomere length
Title | Human MCRS2, a cell-cycle-dependent protein, associates with LPTS/PinX1 and reduces the telomere length |
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Authors | |
Keywords | cDNA Cell-cycle regulation Nucleolar protein Telomerase inhibitor Yeast two-hybrid screening |
Issue Date | 2004 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description |
Citation | Biochemical And Biophysical Research Communications, 2004, v. 316 n. 4, p. 1116-1123 How to Cite? |
Abstract | Human LPTS/PinX1 is a telomerase-inhibitory protein, which binds to the telomere protein Pin2/TRF1 and the catalytic subunit hTERT of telomerase. To explore the proteins that might be involved in the telomerase pathway, we performed a yeast two-hybrid screening with LPTS/PinX1 as the bait. A novel gene, MCRS2, encoding for an isoform of MCRS1/p78 and MSP58 was isolated. The expression of MCRS2 protein is cell-cycle dependent, accumulating in the very early S phase. MCRS2 interacts with LPTS/PinX1 in vitro, in vivo and colocalizes with LPTS/PinX1 in cells. MCRS2 and its amino terminus inhibit telomerase activity in vitro and long-term overexpression of MCRS2 in SMMC-7721 cells results in a gradual and progressive shortening of telomeres. Our findings suggest that MCRS2 might be a linker between telomere maintenance and cell-cycle regulation. © 2004 Elsevier Inc. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/151144 |
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 0.770 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Song, H | en_US |
dc.contributor.author | Li, Y | en_US |
dc.contributor.author | Chen, G | en_US |
dc.contributor.author | Xing, Z | en_US |
dc.contributor.author | Zhao, J | en_US |
dc.contributor.author | Yokoyama, KK | en_US |
dc.contributor.author | Li, T | en_US |
dc.contributor.author | Zhao, M | en_US |
dc.date.accessioned | 2012-06-26T06:17:40Z | - |
dc.date.available | 2012-06-26T06:17:40Z | - |
dc.date.issued | 2004 | en_US |
dc.identifier.citation | Biochemical And Biophysical Research Communications, 2004, v. 316 n. 4, p. 1116-1123 | en_US |
dc.identifier.issn | 0006-291X | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/151144 | - |
dc.description.abstract | Human LPTS/PinX1 is a telomerase-inhibitory protein, which binds to the telomere protein Pin2/TRF1 and the catalytic subunit hTERT of telomerase. To explore the proteins that might be involved in the telomerase pathway, we performed a yeast two-hybrid screening with LPTS/PinX1 as the bait. A novel gene, MCRS2, encoding for an isoform of MCRS1/p78 and MSP58 was isolated. The expression of MCRS2 protein is cell-cycle dependent, accumulating in the very early S phase. MCRS2 interacts with LPTS/PinX1 in vitro, in vivo and colocalizes with LPTS/PinX1 in cells. MCRS2 and its amino terminus inhibit telomerase activity in vitro and long-term overexpression of MCRS2 in SMMC-7721 cells results in a gradual and progressive shortening of telomeres. Our findings suggest that MCRS2 might be a linker between telomere maintenance and cell-cycle regulation. © 2004 Elsevier Inc. All rights reserved. | en_US |
dc.language | eng | en_US |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description | en_US |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | en_US |
dc.subject | cDNA | - |
dc.subject | Cell-cycle regulation | - |
dc.subject | Nucleolar protein | - |
dc.subject | Telomerase inhibitor | - |
dc.subject | Yeast two-hybrid screening | - |
dc.subject.mesh | Base Sequence | en_US |
dc.subject.mesh | Brain - Metabolism | en_US |
dc.subject.mesh | Carcinoma, Hepatocellular - Genetics - Metabolism | en_US |
dc.subject.mesh | Cell Cycle | en_US |
dc.subject.mesh | Cell Cycle Proteins - Genetics - Metabolism | en_US |
dc.subject.mesh | Cell Line, Tumor | en_US |
dc.subject.mesh | Gene Expression Regulation, Neoplastic - Genetics | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Liver - Metabolism | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Nuclear Proteins | en_US |
dc.subject.mesh | Proteins - Genetics - Metabolism | en_US |
dc.subject.mesh | Rna-Binding Proteins | en_US |
dc.subject.mesh | Telomere - Genetics - Metabolism | en_US |
dc.subject.mesh | Tumor Suppressor Proteins - Genetics - Metabolism | en_US |
dc.title | Human MCRS2, a cell-cycle-dependent protein, associates with LPTS/PinX1 and reduces the telomere length | en_US |
dc.type | Article | en_US |
dc.identifier.email | Li, Y:yiliang@hkucc.hku.hk | en_US |
dc.identifier.authority | Li, Y=rp01354 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.bbrc.2004.02.166 | en_US |
dc.identifier.pmid | 15044100 | - |
dc.identifier.scopus | eid_2-s2.0-1642338831 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-1642338831&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 316 | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.spage | 1116 | en_US |
dc.identifier.epage | 1123 | en_US |
dc.identifier.isi | WOS:000220579300022 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Song, H=36984932000 | en_US |
dc.identifier.scopusauthorid | Li, Y=27171876700 | en_US |
dc.identifier.scopusauthorid | Chen, G=15051784500 | en_US |
dc.identifier.scopusauthorid | Xing, Z=7201601380 | en_US |
dc.identifier.scopusauthorid | Zhao, J=7410312823 | en_US |
dc.identifier.scopusauthorid | Yokoyama, KK=7401877315 | en_US |
dc.identifier.scopusauthorid | Li, T=7406375403 | en_US |
dc.identifier.scopusauthorid | Zhao, M=7403535359 | en_US |
dc.identifier.issnl | 0006-291X | - |