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Article: Detection of anti-HIV-1 IgG antibodies in whole saliva by Gacelisa and Western Blot assays

TitleDetection of anti-HIV-1 IgG antibodies in whole saliva by Gacelisa and Western Blot assays
Authors
Matee, MINLyamuya, EFSimon, EMbena, ECKagoma, CSamaranayake, LPScheutz, F
Issue Date1996
PublisherKenya Medical Association.
Citation
East African Medical Journal, 1996, v. 73 n. 5, p. 292-294 How to Cite?
AbstractThe present study, based on 158 HIV seropositives and 167 HIV seronegatives, demonstrates that saliva collected with the Omni-SAL™device and tested with GACELISA (an IgG antibody capture ELISA) is an effective non-invasive alternative to serum for anti-HIV IgG antibody screening. The study also shows that a conventional serum Western blot kit can be used, with slight modifications, for confirmatory testing of saliva specimens. Collecting saliva with the Omni-SAL™ device had a very good acceptance rate among Tanzanian subjects, and although this diagnostic method is not yet known by the general public, 65% of the study participants preferred to give saliva instead of blood for HIV testing.
Persistent Identifierhttp://hdl.handle.net/10722/153971
ISSN
0012-835X
2020 SCImago Journal Rankings: 0.102
ISI Accession Number ID
WOS:A1996VN46900004
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorMatee, MINen_US
dc.contributor.authorLyamuya, EFen_US
dc.contributor.authorSimon, Een_US
dc.contributor.authorMbena, ECen_US
dc.contributor.authorKagoma, Cen_US
dc.contributor.authorSamaranayake, LPen_US
dc.contributor.authorScheutz, Fen_US
dc.date.accessioned2012-08-08T08:22:34Z-
dc.date.available2012-08-08T08:22:34Z-
dc.date.issued1996en_US
dc.identifier.citationEast African Medical Journal, 1996, v. 73 n. 5, p. 292-294en_US
dc.identifier.issn0012-835Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/153971-
dc.description.abstractThe present study, based on 158 HIV seropositives and 167 HIV seronegatives, demonstrates that saliva collected with the Omni-SAL™device and tested with GACELISA (an IgG antibody capture ELISA) is an effective non-invasive alternative to serum for anti-HIV IgG antibody screening. The study also shows that a conventional serum Western blot kit can be used, with slight modifications, for confirmatory testing of saliva specimens. Collecting saliva with the Omni-SAL™ device had a very good acceptance rate among Tanzanian subjects, and although this diagnostic method is not yet known by the general public, 65% of the study participants preferred to give saliva instead of blood for HIV testing.en_US
dc.languageengen_US
dc.publisherKenya Medical Association.-
dc.relation.ispartofEast African Medical Journalen_US
dc.subject.meshAdulten_US
dc.subject.meshBlotting, Western - Methodsen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assay - Methodsen_US
dc.subject.meshHiv Antibodies - Analysisen_US
dc.subject.meshHiv Seropositivity - Blood - Diagnosisen_US
dc.subject.meshHiv-1 - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin G - Analysisen_US
dc.subject.meshPatient Acceptance Of Health Careen_US
dc.subject.meshSaliva - Virologyen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshTanzaniaen_US
dc.titleDetection of anti-HIV-1 IgG antibodies in whole saliva by Gacelisa and Western Blot assaysen_US
dc.typeArticleen_US
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8756029en_US
dc.identifier.scopuseid_2-s2.0-0030138532en_US
dc.identifier.hkuros20268-
dc.identifier.hkuros23391-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030138532&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume73en_US
dc.identifier.issue5en_US
dc.identifier.spage292en_US
dc.identifier.epage294en_US
dc.identifier.isiWOS:A1996VN46900004-
dc.publisher.placeKenyaen_US
dc.publisher.placeKenya-
dc.identifier.scopusauthoridMatee, MIN=35577234100en_US
dc.identifier.scopusauthoridLyamuya, EF=7004124770en_US
dc.identifier.scopusauthoridSimon, E=19235990400en_US
dc.identifier.scopusauthoridMbena, EC=6701842141en_US
dc.identifier.scopusauthoridKagoma, C=6602714859en_US
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_US
dc.identifier.scopusauthoridScheutz, F=7005543084en_US
dc.identifier.issnl0012-835X-

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