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- Publisher Website: 10.1016/j.archoralbio.2004.05.012
- Scopus: eid_2-s2.0-5644227666
- PMID: 15485645
- WOS: WOS:000225233900010
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Article: Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor during healing of allogeneic bone graft
Title | Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor during healing of allogeneic bone graft |
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Authors | |
Keywords | Allogeneic bone graft bFGF DBM IM Revascularization VEGF |
Issue Date | 2004 |
Publisher | Pergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio |
Citation | Archives Of Oral Biology, 2004, v. 49 n. 12, p. 1025-1033 How to Cite? |
Abstract | Recently we reported that basic fibroblast growth factor (bFGF) improved the healing of allogeneic bone grafts. However, the mechanism of action of the bFGF was not known. Therefore, the present study was designed to identify the expression pattern of vascular endothelial growth factor (VEGF) in the presence of bFGF reconstituted in demineralized intramembranous bone matrix (DBM IM) during the healing of allogeneic bone grafts. Eighteen critical size (15 mm × 10 mm) defects were created on rabbit mandibles bilaterally. Three groups of six defects each were grafted with allogeneic bone alone, allogeneic bone and DBM IM, and allogeneic bone and bFGF reconstituted in DBM IM. Three weeks later, the defects were retrieved for immunohistochemistry and in situ hybridization for VEGF. The percentage of positive staining area was quantified by using image analyzer. The increase (517%) in the expression of VEGF mRNA was accompanied by an increase (492%) of immunoreactive VEGF protein in allogeneic bone graft augmented by bFGF reconstituted in DBM IM. A close correlation existed between levels of VEGF production and the amount of newly formed bone. The results show that bFGF reconstituted in DBM IM markedly up-regulated the expression of VEGF in the grafted area. Basic FGF augments the healing of allogeneic bone grafts by enhancing vascularization through the up-regulation of VEGF. © 2004 Elsevier Ltd. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/154550 |
ISSN | 2023 Impact Factor: 2.2 2023 SCImago Journal Rankings: 0.562 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Rabie, ABM | en_HK |
dc.contributor.author | Lu, M | en_HK |
dc.date.accessioned | 2012-08-08T08:26:07Z | - |
dc.date.available | 2012-08-08T08:26:07Z | - |
dc.date.issued | 2004 | en_HK |
dc.identifier.citation | Archives Of Oral Biology, 2004, v. 49 n. 12, p. 1025-1033 | en_HK |
dc.identifier.issn | 0003-9969 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/154550 | - |
dc.description.abstract | Recently we reported that basic fibroblast growth factor (bFGF) improved the healing of allogeneic bone grafts. However, the mechanism of action of the bFGF was not known. Therefore, the present study was designed to identify the expression pattern of vascular endothelial growth factor (VEGF) in the presence of bFGF reconstituted in demineralized intramembranous bone matrix (DBM IM) during the healing of allogeneic bone grafts. Eighteen critical size (15 mm × 10 mm) defects were created on rabbit mandibles bilaterally. Three groups of six defects each were grafted with allogeneic bone alone, allogeneic bone and DBM IM, and allogeneic bone and bFGF reconstituted in DBM IM. Three weeks later, the defects were retrieved for immunohistochemistry and in situ hybridization for VEGF. The percentage of positive staining area was quantified by using image analyzer. The increase (517%) in the expression of VEGF mRNA was accompanied by an increase (492%) of immunoreactive VEGF protein in allogeneic bone graft augmented by bFGF reconstituted in DBM IM. A close correlation existed between levels of VEGF production and the amount of newly formed bone. The results show that bFGF reconstituted in DBM IM markedly up-regulated the expression of VEGF in the grafted area. Basic FGF augments the healing of allogeneic bone grafts by enhancing vascularization through the up-regulation of VEGF. © 2004 Elsevier Ltd. All rights reserved. | en_HK |
dc.language | eng | en_US |
dc.publisher | Pergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio | en_HK |
dc.relation.ispartof | Archives of Oral Biology | en_HK |
dc.subject | Allogeneic bone graft | en_HK |
dc.subject | bFGF | en_HK |
dc.subject | DBM IM | en_HK |
dc.subject | Revascularization | en_HK |
dc.subject | VEGF | en_HK |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Bone Transplantation - Physiology | en_US |
dc.subject.mesh | Bone And Bones - Anatomy & Histology - Physiology | en_US |
dc.subject.mesh | Fibroblast Growth Factor 2 - Physiology | en_US |
dc.subject.mesh | Immunohistochemistry - Methods | en_US |
dc.subject.mesh | In Situ Hybridization - Methods | en_US |
dc.subject.mesh | Neovascularization, Physiologic - Physiology | en_US |
dc.subject.mesh | Rna, Messenger - Analysis | en_US |
dc.subject.mesh | Rabbits | en_US |
dc.subject.mesh | Up-Regulation - Physiology | en_US |
dc.subject.mesh | Vascular Endothelial Growth Factors - Physiology | en_US |
dc.subject.mesh | Wound Healing - Physiology | en_US |
dc.title | Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor during healing of allogeneic bone graft | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Rabie, ABM: rabie@hku.hk | en_HK |
dc.identifier.authority | Rabie, ABM=rp00029 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.archoralbio.2004.05.012 | en_HK |
dc.identifier.pmid | 15485645 | - |
dc.identifier.scopus | eid_2-s2.0-5644227666 | en_HK |
dc.identifier.hkuros | 98722 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-5644227666&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 49 | en_HK |
dc.identifier.issue | 12 | en_HK |
dc.identifier.spage | 1025 | en_HK |
dc.identifier.epage | 1033 | en_HK |
dc.identifier.isi | WOS:000225233900010 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.identifier.scopusauthorid | Rabie, ABM=7007172734 | en_HK |
dc.identifier.scopusauthorid | Lu, M=7402821078 | en_HK |
dc.identifier.issnl | 0003-9969 | - |