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Article: Determination of Internucleotide hJ HN Couplings by the Modified 2D J NN-Correlated [ 15N, 1H] TROSY

TitleDetermination of Internucleotide hJ HN Couplings by the Modified 2D J NN-Correlated [ 15N, 1H] TROSY
Authors
KeywordsHydrogen Bond
J Nn-Correlated [ 15N, 1H] Trosy
Nmr
Trna
Trosy
Issue Date2000
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yjmre
Citation
Journal Of Magnetic Resonance, 2000, v. 147 n. 2, p. 357-360 How to Cite?
AbstractRecent developments in the direct observation of J couplings across hydrogen bonds in proteins and nucleic acids provide additional information for structure and function studies of these molecules by NMR spectroscopy. A J NN-correlated [ 15N, 1H] TROSY experiment proposed by Pervushin et al. (Proc. Natl. Acad. Sci. USA 95, 14147-14151, 1998) can be applied to measure hJ HN in smaller nucleic acids in an E.COSY manner. However, it cannot be effectively applied to large nucleic acids, such as tRNA Trp, since one of the peaks corresponding to a fast relaxing component will be too weak to be observed in the spectra of large molecules. In this Communication, we proposed a modified J NN-correlated [ 15N, 1H] TROSY experiment which enables direct measurement of hJ HN in large nucleic acids. © 2000 Academic Press.
Persistent Identifierhttp://hdl.handle.net/10722/157316
ISSN
2023 Impact Factor: 2.0
2023 SCImago Journal Rankings: 0.593
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYan, Xen_US
dc.contributor.authorKong, Xen_US
dc.contributor.authorXia, Yen_US
dc.contributor.authorSze, KHen_US
dc.contributor.authorZhu, Gen_US
dc.date.accessioned2012-08-08T08:48:52Z-
dc.date.available2012-08-08T08:48:52Z-
dc.date.issued2000en_US
dc.identifier.citationJournal Of Magnetic Resonance, 2000, v. 147 n. 2, p. 357-360en_US
dc.identifier.issn1090-7807en_US
dc.identifier.urihttp://hdl.handle.net/10722/157316-
dc.description.abstractRecent developments in the direct observation of J couplings across hydrogen bonds in proteins and nucleic acids provide additional information for structure and function studies of these molecules by NMR spectroscopy. A J NN-correlated [ 15N, 1H] TROSY experiment proposed by Pervushin et al. (Proc. Natl. Acad. Sci. USA 95, 14147-14151, 1998) can be applied to measure hJ HN in smaller nucleic acids in an E.COSY manner. However, it cannot be effectively applied to large nucleic acids, such as tRNA Trp, since one of the peaks corresponding to a fast relaxing component will be too weak to be observed in the spectra of large molecules. In this Communication, we proposed a modified J NN-correlated [ 15N, 1H] TROSY experiment which enables direct measurement of hJ HN in large nucleic acids. © 2000 Academic Press.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yjmreen_US
dc.relation.ispartofJournal of Magnetic Resonanceen_US
dc.subjectHydrogen Bonden_US
dc.subjectJ Nn-Correlated [ 15N, 1H] Trosyen_US
dc.subjectNmren_US
dc.subjectTrnaen_US
dc.subjectTrosyen_US
dc.titleDetermination of Internucleotide hJ HN Couplings by the Modified 2D J NN-Correlated [ 15N, 1H] TROSYen_US
dc.typeArticleen_US
dc.identifier.emailSze, KH:khsze@hku.hken_US
dc.identifier.authoritySze, KH=rp00785en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/jmre.2000.2221en_US
dc.identifier.pmid11097825-
dc.identifier.scopuseid_2-s2.0-0034573338en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034573338&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume147en_US
dc.identifier.issue2en_US
dc.identifier.spage357en_US
dc.identifier.epage360en_US
dc.identifier.isiWOS:000165909600019-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYan, X=8437853000en_US
dc.identifier.scopusauthoridKong, X=7202794462en_US
dc.identifier.scopusauthoridXia, Y=7403022398en_US
dc.identifier.scopusauthoridSze, KH=7006735061en_US
dc.identifier.scopusauthoridZhu, G=7402633110en_US
dc.identifier.issnl1090-7807-

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