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Article: Octaarginine-modified chitosan as a nonviral gene delivery vector: Properties and in vitro transfection efficiency

TitleOctaarginine-modified chitosan as a nonviral gene delivery vector: Properties and in vitro transfection efficiency
Authors
Zhao, XLi, ZLiu, WLam, WSun, PKao, RYTLuk, KDKLu, WW
KeywordsChitosan
Gene delivery
Nanomedicine
Octaarginine
Issue Date2011
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=1388-0764
Citation
Journal Of Nanoparticle Research, 2011, v. 13 n. 2, p. 693-702 How to Cite?
DOI: http://dx.doi.org/10.1007/s11051-010-0067-3
AbstractProtein transduction domains (PTD) have been identified to have the capacity to facilitate molecular cargo to translocate through cell membrane. This study aims to utilize the cell membrane penetrating ability of octaarginine oligopeptide, a simplified prototype of the PTD, to enhance the transfection efficiency of chitosan. Octaarginine-modified chitosan (R 8-CS) was synthesized as a gene transfer carrier by carbodiimide chemistry. The structure and composition of R 8-CSs were characterized using FTIR and 1H NMR. Agarose gel electrophoresis assay showed that R 8-CS could efficiently condense the DNA. The particle size of R 8-CS/DNA complexes were determined to be around 100-200 nm. The nanoparticle complexes exhibited a spherical and compact morphology. R 8-CS demonstrated higher transfection activity and lower cytotoxicity as compared to the unmodified chitosan and also showed good serum resistance. © Springer Science+Business Media B.V. 2010.
Persistent Identifierhttp://hdl.handle.net/10722/157634
ISSN
1388-0764
2021 Impact Factor: 2.533
2020 SCImago Journal Rankings: 0.453
ISI Accession Number ID
WOS:000287860200024
Funding AgencyGrant Number
Hong Kong RGCHKU7147/07E
Hong Kong Innovation Technology Commission ITF-GHP009-06
HKU200811159133
Funding Information:

This project was partially supported by Hong Kong RGC (HKU7147/07E) and Hong Kong Innovation Technology Commission ITF-GHP 009-06. The authors also acknowledge the support from HKU Seed Funding Programme (200811159133).

References
References in Scopus
Grants
Guanidinylated polyethylenimine (PEI) as nano nonviral vector to transfer human umbilical cord mesenchymal stem cells (UC-hMSC) with BMP2 gene for bone formation

 

DC FieldValueLanguage
dc.contributor.authorZhao, Xen_HK
dc.contributor.authorLi, Zen_HK
dc.contributor.authorLiu, Wen_HK
dc.contributor.authorLam, Wen_HK
dc.contributor.authorSun, Pen_HK
dc.contributor.authorKao, RYTen_HK
dc.contributor.authorLuk, KDKen_HK
dc.contributor.authorLu, WWen_HK
dc.date.accessioned2012-08-08T08:51:50Z-
dc.date.available2012-08-08T08:51:50Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Nanoparticle Research, 2011, v. 13 n. 2, p. 693-702en_HK
dc.identifier.issn1388-0764en_HK
dc.identifier.urihttp://hdl.handle.net/10722/157634-
dc.description.abstractProtein transduction domains (PTD) have been identified to have the capacity to facilitate molecular cargo to translocate through cell membrane. This study aims to utilize the cell membrane penetrating ability of octaarginine oligopeptide, a simplified prototype of the PTD, to enhance the transfection efficiency of chitosan. Octaarginine-modified chitosan (R 8-CS) was synthesized as a gene transfer carrier by carbodiimide chemistry. The structure and composition of R 8-CSs were characterized using FTIR and 1H NMR. Agarose gel electrophoresis assay showed that R 8-CS could efficiently condense the DNA. The particle size of R 8-CS/DNA complexes were determined to be around 100-200 nm. The nanoparticle complexes exhibited a spherical and compact morphology. R 8-CS demonstrated higher transfection activity and lower cytotoxicity as compared to the unmodified chitosan and also showed good serum resistance. © Springer Science+Business Media B.V. 2010.en_HK
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=1388-0764en_HK
dc.relation.ispartofJournal of Nanoparticle Researchen_HK
dc.subjectChitosanen_HK
dc.subjectGene deliveryen_HK
dc.subjectNanomedicineen_HK
dc.subjectOctaarginineen_HK
dc.titleOctaarginine-modified chitosan as a nonviral gene delivery vector: Properties and in vitro transfection efficiencyen_HK
dc.typeArticleen_HK
dc.identifier.emailKao, RYT:rytkao@hkucc.hku.hken_HK
dc.identifier.emailLuk, KDK:hcm21000@hku.hken_HK
dc.identifier.emailLu, WW:wwlu@hku.hken_HK
dc.identifier.authorityKao, RYT=rp00481en_HK
dc.identifier.authorityLuk, KDK=rp00333en_HK
dc.identifier.authorityLu, WW=rp00411en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s11051-010-0067-3en_HK
dc.identifier.scopuseid_2-s2.0-79956153879en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79956153879&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume13en_HK
dc.identifier.issue2en_HK
dc.identifier.spage693en_HK
dc.identifier.epage702en_HK
dc.identifier.isiWOS:000287860200024-
dc.publisher.placeNetherlandsen_HK
dc.relation.projectGuanidinylated polyethylenimine (PEI) as nano nonviral vector to transfer human umbilical cord mesenchymal stem cells (UC-hMSC) with BMP2 gene for bone formation-
dc.identifier.scopusauthoridZhao, X=39162273900en_HK
dc.identifier.scopusauthoridLi, Z=35784563200en_HK
dc.identifier.scopusauthoridLiu, W=7408473555en_HK
dc.identifier.scopusauthoridLam, W=13403256300en_HK
dc.identifier.scopusauthoridSun, P=35254303000en_HK
dc.identifier.scopusauthoridKao, RYT=7101675499en_HK
dc.identifier.scopusauthoridLuk, KDK=7201921573en_HK
dc.identifier.scopusauthoridLu, WW=7404215221en_HK
dc.identifier.citeulike7815357-
dc.identifier.issnl1388-0764-

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