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- Publisher Website: 10.1016/j.jbiomech.2011.09.007
- Scopus: eid_2-s2.0-83555178421
- PMID: 22104169
- WOS: WOS:000299448500016
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Article: Probing the mechanobiological properties of human embryonic stem cells in cardiac differentiation by optical tweezers
Title | Probing the mechanobiological properties of human embryonic stem cells in cardiac differentiation by optical tweezers |
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Authors | |
Keywords | Biomechanics Cardiac differentiation Cell manipulation Human embryonic stem cell Optical tweezers |
Issue Date | 2012 |
Publisher | Pergamon. The Journal's web site is located at http://www.elsevier.com/locate/jbiomech |
Citation | Journal Of Biomechanics, 2012, v. 45 n. 1, p. 123-128 How to Cite? |
Abstract | Human embryonic stem cells (hESC) and hESC-derived cardiomyocytes (hESC-CM) hold great promise for the treatment of cardiovascular diseases. However the mechanobiological properties of hESC and hESC-CM remains elusive. In this paper, we examined the dynamic and static micromechanical properties of hESC and hESC-CM, by manipulating via optical tweezers at the single-cell level. Theoretical approaches were developed to model the dynamic and static mechanical responses of cells during optical stretching. Our experiments showed that the mechanical stiffness of differentiated hESC-CM increased after cardiac differentiation. Such stiffening could associate with increasingly organized myofibrillar assembly that underlines the functional characteristics of hESC-CM. In summary, our findings lay the ground work for using hESC-CMs as models to study mechanical and contractile defects in heart diseases. © 2011 Elsevier Ltd. |
Persistent Identifier | http://hdl.handle.net/10722/163436 |
ISSN | 2023 Impact Factor: 2.4 2023 SCImago Journal Rankings: 0.734 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tan, Y | en_US |
dc.contributor.author | Kong, CW | en_US |
dc.contributor.author | Chen, S | en_US |
dc.contributor.author | Cheng, SH | en_US |
dc.contributor.author | Li, RA | en_US |
dc.contributor.author | Sun, D | en_US |
dc.date.accessioned | 2012-09-05T05:31:19Z | - |
dc.date.available | 2012-09-05T05:31:19Z | - |
dc.date.issued | 2012 | en_US |
dc.identifier.citation | Journal Of Biomechanics, 2012, v. 45 n. 1, p. 123-128 | en_US |
dc.identifier.issn | 0021-9290 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/163436 | - |
dc.description.abstract | Human embryonic stem cells (hESC) and hESC-derived cardiomyocytes (hESC-CM) hold great promise for the treatment of cardiovascular diseases. However the mechanobiological properties of hESC and hESC-CM remains elusive. In this paper, we examined the dynamic and static micromechanical properties of hESC and hESC-CM, by manipulating via optical tweezers at the single-cell level. Theoretical approaches were developed to model the dynamic and static mechanical responses of cells during optical stretching. Our experiments showed that the mechanical stiffness of differentiated hESC-CM increased after cardiac differentiation. Such stiffening could associate with increasingly organized myofibrillar assembly that underlines the functional characteristics of hESC-CM. In summary, our findings lay the ground work for using hESC-CMs as models to study mechanical and contractile defects in heart diseases. © 2011 Elsevier Ltd. | en_US |
dc.language | eng | en_US |
dc.publisher | Pergamon. The Journal's web site is located at http://www.elsevier.com/locate/jbiomech | en_US |
dc.relation.ispartof | Journal of Biomechanics | en_US |
dc.subject | Biomechanics | - |
dc.subject | Cardiac differentiation | - |
dc.subject | Cell manipulation | - |
dc.subject | Human embryonic stem cell | - |
dc.subject | Optical tweezers | - |
dc.subject.mesh | Biomechanics - Physiology | en_US |
dc.subject.mesh | Cardiovascular Diseases - Physiopathology | en_US |
dc.subject.mesh | Cell Differentiation - Physiology | en_US |
dc.subject.mesh | Cells, Cultured | en_US |
dc.subject.mesh | Embryonic Stem Cells - Cytology - Physiology | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Models, Biological | en_US |
dc.subject.mesh | Myocardial Contraction - Physiology | en_US |
dc.subject.mesh | Myocytes, Cardiac - Cytology - Physiology | en_US |
dc.subject.mesh | Myofibrils - Physiology | en_US |
dc.subject.mesh | Optical Tweezers | en_US |
dc.title | Probing the mechanobiological properties of human embryonic stem cells in cardiac differentiation by optical tweezers | en_US |
dc.type | Article | en_US |
dc.identifier.email | Kong, CW:marcokong@hku.hk | en_US |
dc.identifier.email | Li, RA:ronaldli@hkucc.hku.hk | en_US |
dc.identifier.authority | Kong, CW=rp01563 | en_US |
dc.identifier.authority | Li, RA=rp01352 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.jbiomech.2011.09.007 | en_US |
dc.identifier.pmid | 22104169 | - |
dc.identifier.scopus | eid_2-s2.0-83555178421 | en_US |
dc.identifier.hkuros | 212204 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-83555178421&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 45 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 123 | en_US |
dc.identifier.epage | 128 | en_US |
dc.identifier.isi | WOS:000299448500016 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Tan, Y=26028707000 | en_US |
dc.identifier.scopusauthorid | Kong, CW=36784634200 | en_US |
dc.identifier.scopusauthorid | Chen, S=53986175500 | en_US |
dc.identifier.scopusauthorid | Cheng, SH=20233852300 | en_US |
dc.identifier.scopusauthorid | Li, RA=7404724466 | en_US |
dc.identifier.scopusauthorid | Sun, D=7403968591 | en_US |
dc.identifier.citeulike | 10063807 | - |
dc.identifier.issnl | 0021-9290 | - |