RT-SHIV Infected and Passaged in Chinese-Origin Rhesus Macaque

Abstract

目的了解RT-SHIV感染中國恒河猴的感染特點,研究RT-SHIV在中國恒河猴中傳代特點;建立RT-SHIV中國恒河猴動物模型,為評價HIV-1藥物有效性提供動物平臺。方法選擇4只健康恒河猴,其中兩只動物經上肢靜脈感染RT-SHIV病毒,感染急性期采取外周血分離CD8-PBMC,擴增病毒,將新制備的病毒靜脈感染另外兩只中國恒河猴,通過監測血漿病毒載量,CD4+/CD8+比值,CD4+T淋巴細胞和B淋巴細胞的絕對數,了解實驗猴的感染狀態,同時分析病毒RT基因變異情況。結果 4只動物均獲得系統性感染,且傳代動物急性期表現更為強烈,RT基因在感染和傳代的過程中共觀察到3個氨基酸的改變。結論本研究為RT-SHIV中國恒河猴模型的建立提供了基礎信息。 Objective To study the characteristics of RT-SHIV passaging in Chinese rhesus macaques and to establish a RT-SHIV / rhesus animal model providing an animal platform in evaluation of the effectiveness of HIV-1 drugs. Methods Four healthy rhesus macaques selected were negative to SIV，SRV and STLV-1. Two monkeys of them were infected with RT-SHIV intravenously. Blood samples were collected from the infected monkeys at the acute phase and CD8 － PBMC were isolated，and then propagated RT-SHIV. The propagated RT-SHIV with PBMC were injected intravenously into the other two monkeys. Viral load in plasma，CD4 + /CD8 ratio and absolute number of CD4 + T lymphocytes and B lymphocytes were detected，and the RT gene variation was analyzed. Results Systemic infection was established in all of the four rhesus macaques，and the two passaged animals demonstrated more severe changes at the acute phase. There were three amino acid changed during infection and passages. Conclusion The results of our study provide basic information for the establishment of RT-SHIV /Chinese-origin rhesus monkeys models.