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- Publisher Website: 10.1016/j.cellimm.2012.02.016
- Scopus: eid_2-s2.0-84860595423
- PMID: 22483852
- WOS: WOS:000304509400010
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Article: Over-expression of Hlx homeobox gene in DC2.4 dendritic cell enhances its maturation and antigen presentation
Title | Over-expression of Hlx homeobox gene in DC2.4 dendritic cell enhances its maturation and antigen presentation |
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Authors | |
Keywords | Antigen presenting Dendritic cell Genetic modification H2.0-like homebox |
Issue Date | 2012 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/ycimm |
Citation | Cellular Immunology, 2012, v. 275 n. 1-2, p. 61-68 How to Cite? |
Abstract | Hlx as a Th1-specific transcription factor, it appears to drive maturation of Th1 and IFN-γ secretion in cooperation with T-bet. In this study, we established a stable Hlx-over-expressed dendritic cell line (DC2.4/Hlx), and investigated the possible effect of Hlx gene on maturation of dendritic cell-line (DC2.4). Results shown that over-expressed Hlx in DC2.4 up-regulated the transcription and expression of IFN-γ, increased the expression of maturation makers including CD40, CD80, CD86, MHC-I and MHC-II. Functional assay for DC2.4/Hlx showed that over-expressed Hlx increased the expression level of interleukin-12 in the supernatant and decreased DC endocytosis when cells were incubated in vitro. Furthermore, using a syngeneic T cell activation model, we found that DC2.4/Hlx could obviously present ovalbumin (OVA) antigen to T cell in OVA pre-immunized mice. |
Persistent Identifier | http://hdl.handle.net/10722/164842 |
ISSN | 2023 Impact Factor: 3.7 2023 SCImago Journal Rankings: 1.011 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Shotorbani, SS | - |
dc.contributor.author | He, Z | - |
dc.contributor.author | Yang, H | - |
dc.contributor.author | Sun, Q | - |
dc.contributor.author | Xu, Y | - |
dc.contributor.author | Su, Z | - |
dc.contributor.author | Xue, Y | - |
dc.contributor.author | Zheng, D | - |
dc.contributor.author | Zhang, Y | - |
dc.contributor.author | Wang, S | - |
dc.contributor.author | Shao, Q | - |
dc.contributor.author | Lu, L | - |
dc.contributor.author | Xu, H | - |
dc.date.accessioned | 2012-09-20T08:10:47Z | - |
dc.date.available | 2012-09-20T08:10:47Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | Cellular Immunology, 2012, v. 275 n. 1-2, p. 61-68 | - |
dc.identifier.issn | 0008-8749 | - |
dc.identifier.uri | http://hdl.handle.net/10722/164842 | - |
dc.description.abstract | Hlx as a Th1-specific transcription factor, it appears to drive maturation of Th1 and IFN-γ secretion in cooperation with T-bet. In this study, we established a stable Hlx-over-expressed dendritic cell line (DC2.4/Hlx), and investigated the possible effect of Hlx gene on maturation of dendritic cell-line (DC2.4). Results shown that over-expressed Hlx in DC2.4 up-regulated the transcription and expression of IFN-γ, increased the expression of maturation makers including CD40, CD80, CD86, MHC-I and MHC-II. Functional assay for DC2.4/Hlx showed that over-expressed Hlx increased the expression level of interleukin-12 in the supernatant and decreased DC endocytosis when cells were incubated in vitro. Furthermore, using a syngeneic T cell activation model, we found that DC2.4/Hlx could obviously present ovalbumin (OVA) antigen to T cell in OVA pre-immunized mice. | - |
dc.language | eng | - |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/ycimm | - |
dc.relation.ispartof | Cellular Immunology | - |
dc.subject | Antigen presenting | - |
dc.subject | Dendritic cell | - |
dc.subject | Genetic modification | - |
dc.subject | H2.0-like homebox | - |
dc.title | Over-expression of Hlx homeobox gene in DC2.4 dendritic cell enhances its maturation and antigen presentation | - |
dc.type | Article | - |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1090-2163 (Electronic) 0008-8749 (Linkin&volume=275&issue=1-2&spage=61&epage=8&date=2012&atitle=Over-expression+of+Hlx+homeobox+gene+in+DC2.4+dendritic+cell+enhances+its+maturation+and+antigen+presentation | en_US |
dc.identifier.email | Lu, L: liweilu@hkucc.hku.hk | - |
dc.identifier.authority | Lu, L=rp00477 | - |
dc.identifier.doi | 10.1016/j.cellimm.2012.02.016 | - |
dc.identifier.pmid | 22483852 | - |
dc.identifier.scopus | eid_2-s2.0-84860595423 | - |
dc.identifier.hkuros | 208157 | - |
dc.identifier.volume | 275 | - |
dc.identifier.issue | 1-2 | - |
dc.identifier.spage | 61 | - |
dc.identifier.epage | 68 | - |
dc.identifier.isi | WOS:000304509400010 | - |
dc.publisher.place | United States | - |
dc.identifier.citeulike | 10469753 | - |
dc.identifier.issnl | 0008-8749 | - |