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Article: Measurement of dimethylglycine in biological fluids

TitleMeasurement of dimethylglycine in biological fluids
Authors
Porter, DHLin, MWagner, C
Keywordsamino acids
dimethylglycine analysis
dimethylglycine dehydrogenase
enzymatic assay
HPLC
plasma
urine
Issue Date1985
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yabio
Citation
Analytical Biochemistry, 1985, v. 151 n. 2, p. 299-303 How to Cite?
DOI: http://dx.doi.org/10.1016/0003-2697(85)90179-4
AbstractThe method of quantitating N,N-dimethylglycine involves cation-exchange high-performance liquid chromatography and detection of dimethylglycine with dimethylglycine dehydrogenase. Dimethylglycine was added to plasma and urine and samples were assayed for dimethylglycine. Plasma and urine to which no dimethylglycine was added were also assayed. Recoveries of added dimethylglycine were 99 to 104% with no endogenous dimethylglycine found in rat plasma or normal human urine. The human plasma used contained a small amount of endogenous dimethylglycine. The cation-exchange chromatography separates dimethylglycine from other compounds which can serve as substrates for dimethylglycine dehydrogenase. Repeatability of the assay is ±10%. Using this method we have identified dimethylglycine in the urine of a 1-month-old female human patient.
Persistent Identifierhttp://hdl.handle.net/10722/167467
ISSN
0003-2697
2021 Impact Factor: 3.191
2020 SCImago Journal Rankings: 0.633
ISI Accession Number ID
WOS:A1985AWR2800012

 

DC FieldValueLanguage
dc.contributor.authorPorter, DHen_US
dc.contributor.authorLin, Men_US
dc.contributor.authorWagner, Cen_US
dc.date.accessioned2012-10-08T03:07:23Z-
dc.date.available2012-10-08T03:07:23Z-
dc.date.issued1985en_US
dc.identifier.citationAnalytical Biochemistry, 1985, v. 151 n. 2, p. 299-303en_US
dc.identifier.issn0003-2697en_US
dc.identifier.urihttp://hdl.handle.net/10722/167467-
dc.description.abstractThe method of quantitating N,N-dimethylglycine involves cation-exchange high-performance liquid chromatography and detection of dimethylglycine with dimethylglycine dehydrogenase. Dimethylglycine was added to plasma and urine and samples were assayed for dimethylglycine. Plasma and urine to which no dimethylglycine was added were also assayed. Recoveries of added dimethylglycine were 99 to 104% with no endogenous dimethylglycine found in rat plasma or normal human urine. The human plasma used contained a small amount of endogenous dimethylglycine. The cation-exchange chromatography separates dimethylglycine from other compounds which can serve as substrates for dimethylglycine dehydrogenase. Repeatability of the assay is ±10%. Using this method we have identified dimethylglycine in the urine of a 1-month-old female human patient.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yabioen_US
dc.relation.ispartofAnalytical Biochemistryen_US
dc.subjectamino acids-
dc.subjectdimethylglycine analysis-
dc.subjectdimethylglycine dehydrogenase-
dc.subjectenzymatic assay-
dc.subjectHPLC-
dc.subjectplasma-
dc.subjecturine-
dc.subject.meshCarbon Radioisotopesen_US
dc.subject.meshChromatography, High Pressure Liquid - Methodsen_US
dc.subject.meshDimethylglycine Dehydrogenaseen_US
dc.subject.meshGlycine - Analogs & Derivativesen_US
dc.subject.meshHumansen_US
dc.subject.meshMitochondrial Proteinsen_US
dc.subject.meshOxidoreductases, N-Demethylating - Metabolismen_US
dc.subject.meshRadioisotope Dilution Techniqueen_US
dc.subject.meshSarcosine - Analogs & Derivatives - Blood - Urineen_US
dc.subject.meshSubstrate Specificityen_US
dc.titleMeasurement of dimethylglycine in biological fluidsen_US
dc.typeArticleen_US
dc.identifier.emailLin, M:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, M=rp00746en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0003-2697(85)90179-4-
dc.identifier.pmid2420226-
dc.identifier.scopuseid_2-s2.0-0022335230en_US
dc.identifier.volume151en_US
dc.identifier.issue2en_US
dc.identifier.spage299en_US
dc.identifier.epage303en_US
dc.identifier.isiWOS:A1985AWR2800012-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridPorter, DH=36847248400en_US
dc.identifier.scopusauthoridLin, M=7404816359en_US
dc.identifier.scopusauthoridWagner, C=7402148945en_US
dc.identifier.issnl0003-2697-

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