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- Publisher Website: 10.1016/0014-4827(86)90339-3
- Scopus: eid_2-s2.0-0022590855
- PMID: 3002821
- WOS: WOS:A1986AYY0600005
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Article: Revertants of Ha-MuSV-transformed MDCK cells express reduced levels of p21 and possess a more normal phenotype
Title | Revertants of Ha-MuSV-transformed MDCK cells express reduced levels of p21 and possess a more normal phenotype |
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Authors | |
Issue Date | 1986 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexcr |
Citation | Experimental Cell Research, 1986, v. 162 n. 2, p. 335-346 How to Cite? |
Abstract | Four subclones of the originally cloned Harvey murine sarcoma virus-transformed Madin Darby canine kidney (MDCK) cells have been isolated. These subclones fall into two general classes. Two subclones have a fibroblastic morphology, have lost the growth requirement for prostaglandin E 1 (PGE 1), do not respond to glucagon or vasopressin, and, in general, appear transformed. Two other subclones have epithelioid morphologies, are growth-stimulated by PGE 1, respond to vasopressin with an increase in intracellular cAMP. We propose that these cells represent revertants to a more non-transformed phenotype. Unlike normal cells, however, these revertants grow under anchorage-independent conditions, express detectable but reduceced amounts of the transforming gene product, p21, and grow in nude mice. The appearance of such revertants may be one cause of the observed heterogeneity of tumor cells. |
Persistent Identifier | http://hdl.handle.net/10722/167470 |
ISSN | 2023 Impact Factor: 3.3 2023 SCImago Journal Rankings: 0.947 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Darfler, FJ | en_US |
dc.contributor.author | Shih, TY | en_US |
dc.contributor.author | Lin, MC | en_US |
dc.date.accessioned | 2012-10-08T03:07:24Z | - |
dc.date.available | 2012-10-08T03:07:24Z | - |
dc.date.issued | 1986 | en_US |
dc.identifier.citation | Experimental Cell Research, 1986, v. 162 n. 2, p. 335-346 | en_US |
dc.identifier.issn | 0014-4827 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/167470 | - |
dc.description.abstract | Four subclones of the originally cloned Harvey murine sarcoma virus-transformed Madin Darby canine kidney (MDCK) cells have been isolated. These subclones fall into two general classes. Two subclones have a fibroblastic morphology, have lost the growth requirement for prostaglandin E 1 (PGE 1), do not respond to glucagon or vasopressin, and, in general, appear transformed. Two other subclones have epithelioid morphologies, are growth-stimulated by PGE 1, respond to vasopressin with an increase in intracellular cAMP. We propose that these cells represent revertants to a more non-transformed phenotype. Unlike normal cells, however, these revertants grow under anchorage-independent conditions, express detectable but reduceced amounts of the transforming gene product, p21, and grow in nude mice. The appearance of such revertants may be one cause of the observed heterogeneity of tumor cells. | en_US |
dc.language | eng | en_US |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexcr | en_US |
dc.relation.ispartof | Experimental Cell Research | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Cell Differentiation | en_US |
dc.subject.mesh | Cell Division - Drug Effects | en_US |
dc.subject.mesh | Cell Line | en_US |
dc.subject.mesh | Cell Transformation, Viral | en_US |
dc.subject.mesh | Culture Media | en_US |
dc.subject.mesh | Cyclic Amp - Metabolism | en_US |
dc.subject.mesh | Dinoprostone | en_US |
dc.subject.mesh | Dogs | en_US |
dc.subject.mesh | Glucagon - Pharmacology | en_US |
dc.subject.mesh | Growth Substances | en_US |
dc.subject.mesh | Harvey Murine Sarcoma Virus - Genetics | en_US |
dc.subject.mesh | Mutation | en_US |
dc.subject.mesh | Oncogene Proteins, Viral - Genetics - Metabolism | en_US |
dc.subject.mesh | Prostaglandins E - Metabolism | en_US |
dc.subject.mesh | Sarcoma, Experimental - Genetics - Microbiology - Pathology | en_US |
dc.subject.mesh | Vasopressins - Pharmacology | en_US |
dc.title | Revertants of Ha-MuSV-transformed MDCK cells express reduced levels of p21 and possess a more normal phenotype | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lin, MC:mcllin@hkucc.hku.hk | en_US |
dc.identifier.authority | Lin, MC=rp00746 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/0014-4827(86)90339-3 | - |
dc.identifier.pmid | 3002821 | - |
dc.identifier.scopus | eid_2-s2.0-0022590855 | en_US |
dc.identifier.volume | 162 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 335 | en_US |
dc.identifier.epage | 346 | en_US |
dc.identifier.isi | WOS:A1986AYY0600005 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Darfler, FJ=6603120994 | en_US |
dc.identifier.scopusauthorid | Shih, TY=7202664631 | en_US |
dc.identifier.scopusauthorid | Lin, MC=7404816359 | en_US |
dc.identifier.issnl | 0014-4827 | - |