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- Publisher Website: 10.1021/ac981351n
- Scopus: eid_2-s2.0-0033168984
- PMID: 10405604
- WOS: WOS:000081265600029
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Article: Sequencing of argentinated peptides by means of electrospray tandem mass spectrometry
Title | Sequencing of argentinated peptides by means of electrospray tandem mass spectrometry |
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Authors | |
Issue Date | 1999 |
Publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/ac |
Citation | Analytical Chemistry, 1999, v. 71 n. 13, p. 2364-2372 How to Cite? |
Abstract | A strategy for semiautomatic sequencing of argentinated (silver- containing) oligopeptides has been developed. Sequencing is based on a search algorithm that identifies a triplet peak relationship in a product ion spectrum of the [M + Ag] + ion of an oligopeptide. The ions that constitute a triplet are [b(n) + OH + Ag] +, [b(n) - H+Ag] +, and [a(n) - H + Ag] +, and [a(n) - H + Ag] +, which are separated by 18 and 28 m/z units, respectively. The difference in the m/z values of adjacent triplets identifies the residue that is 'cleaved'. Observation of the [y(n) + H + Ag] + ion containing the cleaved residue confirms the assignment. Sequencing of argentinated tryptic peptides may prove useful for automated proteome analysis via the sequence tag method. |
Persistent Identifier | http://hdl.handle.net/10722/167617 |
ISSN | 2023 Impact Factor: 6.7 2023 SCImago Journal Rankings: 1.621 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chu, IK | en_US |
dc.contributor.author | Guo, X | en_US |
dc.contributor.author | Lau, TI | en_US |
dc.contributor.author | Siu, KWM | en_US |
dc.date.accessioned | 2012-10-08T03:09:06Z | - |
dc.date.available | 2012-10-08T03:09:06Z | - |
dc.date.issued | 1999 | en_US |
dc.identifier.citation | Analytical Chemistry, 1999, v. 71 n. 13, p. 2364-2372 | en_US |
dc.identifier.issn | 0003-2700 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/167617 | - |
dc.description.abstract | A strategy for semiautomatic sequencing of argentinated (silver- containing) oligopeptides has been developed. Sequencing is based on a search algorithm that identifies a triplet peak relationship in a product ion spectrum of the [M + Ag] + ion of an oligopeptide. The ions that constitute a triplet are [b(n) + OH + Ag] +, [b(n) - H+Ag] +, and [a(n) - H + Ag] +, and [a(n) - H + Ag] +, which are separated by 18 and 28 m/z units, respectively. The difference in the m/z values of adjacent triplets identifies the residue that is 'cleaved'. Observation of the [y(n) + H + Ag] + ion containing the cleaved residue confirms the assignment. Sequencing of argentinated tryptic peptides may prove useful for automated proteome analysis via the sequence tag method. | en_US |
dc.language | eng | en_US |
dc.publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/ac | en_US |
dc.relation.ispartof | Analytical Chemistry | en_US |
dc.subject.mesh | Amino Acid Sequence | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Cattle | en_US |
dc.subject.mesh | Hydrolysis | en_US |
dc.subject.mesh | Mass Spectrometry | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Oligopeptides - Analysis - Chemistry | en_US |
dc.subject.mesh | Silver - Chemistry | en_US |
dc.subject.mesh | Trypsin | en_US |
dc.title | Sequencing of argentinated peptides by means of electrospray tandem mass spectrometry | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chu, IK:ivankchu@hku.hk | en_US |
dc.identifier.authority | Chu, IK=rp00683 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1021/ac981351n | en_US |
dc.identifier.pmid | 10405604 | - |
dc.identifier.scopus | eid_2-s2.0-0033168984 | en_US |
dc.identifier.volume | 71 | en_US |
dc.identifier.issue | 13 | en_US |
dc.identifier.spage | 2364 | en_US |
dc.identifier.epage | 2372 | en_US |
dc.identifier.isi | WOS:000081265600029 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Chu, IK=7103327484 | en_US |
dc.identifier.scopusauthorid | Guo, X=36725887400 | en_US |
dc.identifier.scopusauthorid | Lau, TI=20935027800 | en_US |
dc.identifier.scopusauthorid | Siu, KWM=35312218500 | en_US |
dc.identifier.issnl | 0003-2700 | - |