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- Publisher Website: 10.1002/jcb.21477
- Scopus: eid_2-s2.0-40349113287
- PMID: 18027883
- WOS: WOS:000253873800004
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Article: Oligodeoxynucleotide decoy therapy blocks type 1 procollagen transcription and the prolyl hydroxylase β subunit translation
Title | Oligodeoxynucleotide decoy therapy blocks type 1 procollagen transcription and the prolyl hydroxylase β subunit translation |
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Authors | |
Keywords | Collagen synthesis Gene therapy Oligonucleotide decoy Proteomics |
Issue Date | 2008 |
Publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503 |
Citation | Journal Of Cellular Biochemistry, 2008, v. 103 n. 4, p. 1066-1075 How to Cite? |
Abstract | Persistent transforming growth factor-β1 (TGF-β1) exposure to lungs increases type 1 collagen synthesis and deposition resulting in excess fibrosis which leads to morbidity and possibly death. We now report using human embryonic lung fibroblasts in the presence of TGF-β1, a novel double-stranded (ds) DNA decoy with phosphorothioate (PT) linkages, containing the TGF-β cis-element found in the distal promoter region of the COL1A1 gene which silences COL1A1 gene expression. In a cell-free protein translation system, we have previously reported that collagen synthesis was inhibited by disulfide isomerase, the prolyl-4-hydroxylase (P-4-H) β subunit. By comparative proteomics dsdecoy therapy increased the levels of disulfide isomerase, the P-4-H β subunit. These findings taken together support the notion that the dsdecoy inhibits type 1 collagen synthesis at both the transcriptional and translational levels. © 2007 Wiley-Liss, Inc. |
Persistent Identifier | http://hdl.handle.net/10722/168279 |
ISSN | 2023 Impact Factor: 3.0 2023 SCImago Journal Rankings: 0.768 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Lok, CN | en_US |
dc.contributor.author | Ehrlich, HP | en_US |
dc.contributor.author | White, SL | en_US |
dc.contributor.author | Buttolph, TR | en_US |
dc.contributor.author | Cutroneo, KR | en_US |
dc.contributor.author | Chiu, JF | en_US |
dc.date.accessioned | 2012-10-08T03:16:58Z | - |
dc.date.available | 2012-10-08T03:16:58Z | - |
dc.date.issued | 2008 | en_US |
dc.identifier.citation | Journal Of Cellular Biochemistry, 2008, v. 103 n. 4, p. 1066-1075 | en_US |
dc.identifier.issn | 0730-2312 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/168279 | - |
dc.description.abstract | Persistent transforming growth factor-β1 (TGF-β1) exposure to lungs increases type 1 collagen synthesis and deposition resulting in excess fibrosis which leads to morbidity and possibly death. We now report using human embryonic lung fibroblasts in the presence of TGF-β1, a novel double-stranded (ds) DNA decoy with phosphorothioate (PT) linkages, containing the TGF-β cis-element found in the distal promoter region of the COL1A1 gene which silences COL1A1 gene expression. In a cell-free protein translation system, we have previously reported that collagen synthesis was inhibited by disulfide isomerase, the prolyl-4-hydroxylase (P-4-H) β subunit. By comparative proteomics dsdecoy therapy increased the levels of disulfide isomerase, the P-4-H β subunit. These findings taken together support the notion that the dsdecoy inhibits type 1 collagen synthesis at both the transcriptional and translational levels. © 2007 Wiley-Liss, Inc. | en_US |
dc.language | eng | en_US |
dc.publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503 | en_US |
dc.relation.ispartof | Journal of Cellular Biochemistry | en_US |
dc.subject | Collagen synthesis | - |
dc.subject | Gene therapy | - |
dc.subject | Oligonucleotide decoy | - |
dc.subject | Proteomics | - |
dc.subject.mesh | Cell Line | en_US |
dc.subject.mesh | Collagen Type I - Biosynthesis - Genetics | en_US |
dc.subject.mesh | Embryo, Mammalian - Cytology | en_US |
dc.subject.mesh | Fibroblasts - Metabolism | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Lung - Cytology | en_US |
dc.subject.mesh | Oligonucleotides - Genetics - Pharmacology | en_US |
dc.subject.mesh | Oligonucleotides, Antisense - Genetics - Pharmacology | en_US |
dc.subject.mesh | Procollagen-Proline Dioxygenase - Biosynthesis | en_US |
dc.subject.mesh | Promoter Regions, Genetic | en_US |
dc.subject.mesh | Protein Biosynthesis | en_US |
dc.subject.mesh | Transcription, Genetic | en_US |
dc.subject.mesh | Transforming Growth Factor Beta1 - Genetics - Pharmacology | en_US |
dc.title | Oligodeoxynucleotide decoy therapy blocks type 1 procollagen transcription and the prolyl hydroxylase β subunit translation | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lok, CN:cnlok@hku.hk | en_US |
dc.identifier.authority | Lok, CN=rp00752 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1002/jcb.21477 | en_US |
dc.identifier.pmid | 18027883 | - |
dc.identifier.scopus | eid_2-s2.0-40349113287 | en_US |
dc.identifier.hkuros | 141592 | - |
dc.identifier.volume | 103 | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.spage | 1066 | en_US |
dc.identifier.epage | 1075 | en_US |
dc.identifier.isi | WOS:000253873800004 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Lok, CN=7006410829 | en_US |
dc.identifier.scopusauthorid | Ehrlich, HP=7102414814 | en_US |
dc.identifier.scopusauthorid | White, SL=7404080473 | en_US |
dc.identifier.scopusauthorid | Buttolph, TR=13104427200 | en_US |
dc.identifier.scopusauthorid | Cutroneo, KR=7005730844 | en_US |
dc.identifier.scopusauthorid | Chiu, JF=7201501692 | en_US |
dc.identifier.issnl | 0730-2312 | - |