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Article: MiR-637 maintains the balance between adipocytes and osteoblasts by directly targeting Osterix
Title | MiR-637 maintains the balance between adipocytes and osteoblasts by directly targeting Osterix |
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Authors | |
Issue Date | 2011 |
Publisher | American Society for Cell Biology. The Journal's web site is located at http://www.molbiolcell.org/ |
Citation | Molecular Biology Of The Cell, 2011, v. 22 n. 21, p. 3955-3961 How to Cite? |
Abstract | Bone development is dynamically regulated by homeostasis, in which a balance between adipocytes and osteoblasts is maintained. Disruption of this differentiation balance leads to various bone-related metabolic diseases, including osteoporosis. In the present study, a primate-specific microRNA (miR-637) was found to be involved in the differentiation of human mesenchymal stem cells (hMSCs). Our preliminary data indicated that miR-637 suppressed the growth of hMSCs and induced S-phase arrest. Expression of miR-637 was increased during adipocyte differentiation (AD), whereas it was decreased during osteoblast differentiation (OS), which suggests miR-637 could act as a mediator of adipoosteogenic differentiation. Osterix (Osx), a significant transcription factor of osteoblasts, was shown to be a direct target of miR-637, which significantly enhanced AD and suppressed OS in hMSCs through direct suppression of Osx expression. Furthermore, miR-637 also significantly enhanced de novo adipogenesis in nude mice. In conclusion, our data indicated that the expression of miR-637 was indispensable for maintaining the balance of adipocytes and osteoblasts. Disruption of miR-637 expression patterns leads to irreversible damage to the balance of differentiation in bone marrow. © 2011 Zhang et al. |
Persistent Identifier | http://hdl.handle.net/10722/168582 |
ISSN | 2023 Impact Factor: 3.1 2023 SCImago Journal Rankings: 1.566 |
PubMed Central ID | |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zhang, JF | en_US |
dc.contributor.author | Fu, WM | en_US |
dc.contributor.author | He, ML | en_US |
dc.contributor.author | Wang, H | en_US |
dc.contributor.author | Wang, WM | en_US |
dc.contributor.author | Yu, SC | en_US |
dc.contributor.author | Bian, XW | en_US |
dc.contributor.author | Zhou, J | en_US |
dc.contributor.author | Lin, MCM | en_US |
dc.contributor.author | Lu, G | en_US |
dc.contributor.author | Poon, WS | en_US |
dc.contributor.author | Kung, HF | en_US |
dc.date.accessioned | 2012-10-08T03:21:05Z | - |
dc.date.available | 2012-10-08T03:21:05Z | - |
dc.date.issued | 2011 | en_US |
dc.identifier.citation | Molecular Biology Of The Cell, 2011, v. 22 n. 21, p. 3955-3961 | en_US |
dc.identifier.issn | 1059-1524 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/168582 | - |
dc.description.abstract | Bone development is dynamically regulated by homeostasis, in which a balance between adipocytes and osteoblasts is maintained. Disruption of this differentiation balance leads to various bone-related metabolic diseases, including osteoporosis. In the present study, a primate-specific microRNA (miR-637) was found to be involved in the differentiation of human mesenchymal stem cells (hMSCs). Our preliminary data indicated that miR-637 suppressed the growth of hMSCs and induced S-phase arrest. Expression of miR-637 was increased during adipocyte differentiation (AD), whereas it was decreased during osteoblast differentiation (OS), which suggests miR-637 could act as a mediator of adipoosteogenic differentiation. Osterix (Osx), a significant transcription factor of osteoblasts, was shown to be a direct target of miR-637, which significantly enhanced AD and suppressed OS in hMSCs through direct suppression of Osx expression. Furthermore, miR-637 also significantly enhanced de novo adipogenesis in nude mice. In conclusion, our data indicated that the expression of miR-637 was indispensable for maintaining the balance of adipocytes and osteoblasts. Disruption of miR-637 expression patterns leads to irreversible damage to the balance of differentiation in bone marrow. © 2011 Zhang et al. | en_US |
dc.language | eng | en_US |
dc.publisher | American Society for Cell Biology. The Journal's web site is located at http://www.molbiolcell.org/ | en_US |
dc.relation.ispartof | Molecular Biology of the Cell | en_US |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject.mesh | Adipocytes - Metabolism - Physiology | en_US |
dc.subject.mesh | Adipogenesis - Genetics | en_US |
dc.subject.mesh | Adipose Tissue - Anatomy & Histology - Physiology | en_US |
dc.subject.mesh | Adult | en_US |
dc.subject.mesh | Alkaline Phosphatase - Metabolism | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Cell Cycle Checkpoints | en_US |
dc.subject.mesh | Cell Proliferation | en_US |
dc.subject.mesh | Cells, Cultured | en_US |
dc.subject.mesh | Enzyme Assays | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Gene Expression | en_US |
dc.subject.mesh | Gene Expression Regulation | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Mesenchymal Stem Cells | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Mice, Nude | en_US |
dc.subject.mesh | Micrornas - Genetics - Metabolism | en_US |
dc.subject.mesh | Middle Aged | en_US |
dc.subject.mesh | Osteoblasts - Metabolism - Physiology | en_US |
dc.subject.mesh | Transcription Factors - Genetics - Metabolism | en_US |
dc.title | MiR-637 maintains the balance between adipocytes and osteoblasts by directly targeting Osterix | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lin, MCM:mcllin@hkucc.hku.hk | en_US |
dc.identifier.authority | Lin, MCM=rp00746 | en_US |
dc.description.nature | published_or_final_version | en_US |
dc.identifier.doi | 10.1091/mbc.E11-04-0356 | en_US |
dc.identifier.pmid | 21880893 | - |
dc.identifier.pmcid | PMC3204058 | - |
dc.identifier.scopus | eid_2-s2.0-80655125005 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-80655125005&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 22 | en_US |
dc.identifier.issue | 21 | en_US |
dc.identifier.spage | 3955 | en_US |
dc.identifier.epage | 3961 | en_US |
dc.identifier.isi | WOS:000296603300012 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Zhang, JF=13007942600 | en_US |
dc.identifier.scopusauthorid | Fu, WM=52463551200 | en_US |
dc.identifier.scopusauthorid | He, ML=35080389700 | en_US |
dc.identifier.scopusauthorid | Wang, H=8748902500 | en_US |
dc.identifier.scopusauthorid | Wang, WM=54409697600 | en_US |
dc.identifier.scopusauthorid | Yu, SC=25629204700 | en_US |
dc.identifier.scopusauthorid | Bian, XW=7103023096 | en_US |
dc.identifier.scopusauthorid | Zhou, J=7405550537 | en_US |
dc.identifier.scopusauthorid | Lin, MCM=7404816359 | en_US |
dc.identifier.scopusauthorid | Lu, G=36619108300 | en_US |
dc.identifier.scopusauthorid | Poon, WS=7103025507 | en_US |
dc.identifier.scopusauthorid | Kung, HF=7402514190 | en_US |
dc.identifier.issnl | 1059-1524 | - |