File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Rgs19 regulates mouse palatal fusion by modulating cell proliferation and apoptosis in the MEE

TitleRgs19 regulates mouse palatal fusion by modulating cell proliferation and apoptosis in the MEE
Authors
KeywordsApoptosis
Genome Wide Screening
Palatal Fusion
Palatogenesis
Rgs19
Issue Date2012
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/modo
Citation
Mechanisms Of Development, 2012, v. 129 n. 9-12, p. 244-254 How to Cite?
AbstractPalatal development is one of the critical events in craniofacial morphogenesis. During fusion of the palatal shelves, removal of the midline epithelial seam (MES) is a fundamental process for achieving proper morphogenesis of the palate. The reported mechanisms for removing the MES are the processes of apoptosis, migration or general epithelial-to-mesenchymal transition (EMT) through modulations of various signaling molecules including Wnt signaling. RGS19, a regulator of the G protein signaling (RGS) family, interacts selectively with the specific α subunits of the G proteins (Gαi, Gαq) and enhances their GTPase activity. Rgs19 was reported to be a modulator of the Wnt signaling pathway. In mouse palatogenesis, the restricted epithelial expression pattern of Rgs19 was examined in the palatal shelves, where expression of Wnt11 was observed. Based on these specific expression patterns of Rgs19 in the palatal shelves, the present study examined the detailed developmental function of Rgs19 using AS-ODN treatments during in vitro palate organ cultivations as a loss-of-function study. After the knockdown of Rgs19, the morphological changes in the palatal shelves was examined carefully using a computer-aided three dimensional reconstruction method and the altered expression patterns of related signaling molecules were evaluated using genome wide screening methods. RT-qPCR and in situ hybridization methods were also used to confirm these array results. These morphological and molecular examinations suggested that Rgs19 plays important roles in palatal fusion through the degradation of MES via activation of the palatal fusion related and apoptotic related genes. Overall, inhibition of the proliferation related and Wnt responsive genes by Rgs19 are required for proper palatal fusion. © 2012 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/169594
ISSN
2022 Impact Factor: 2.6
2020 SCImago Journal Rankings: 0.890
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSohn, WJen_US
dc.contributor.authorJi, YRen_US
dc.contributor.authorKim, HSen_US
dc.contributor.authorGwon, GJen_US
dc.contributor.authorChae, YMen_US
dc.contributor.authorAn, CHen_US
dc.contributor.authorPark, Hden_US
dc.contributor.authorJung, HSen_US
dc.contributor.authorRyoo, ZYen_US
dc.contributor.authorLee, Sen_US
dc.contributor.authorKim, JYen_US
dc.date.accessioned2012-10-25T04:53:17Z-
dc.date.available2012-10-25T04:53:17Z-
dc.date.issued2012en_US
dc.identifier.citationMechanisms Of Development, 2012, v. 129 n. 9-12, p. 244-254en_US
dc.identifier.issn0925-4773en_US
dc.identifier.urihttp://hdl.handle.net/10722/169594-
dc.description.abstractPalatal development is one of the critical events in craniofacial morphogenesis. During fusion of the palatal shelves, removal of the midline epithelial seam (MES) is a fundamental process for achieving proper morphogenesis of the palate. The reported mechanisms for removing the MES are the processes of apoptosis, migration or general epithelial-to-mesenchymal transition (EMT) through modulations of various signaling molecules including Wnt signaling. RGS19, a regulator of the G protein signaling (RGS) family, interacts selectively with the specific α subunits of the G proteins (Gαi, Gαq) and enhances their GTPase activity. Rgs19 was reported to be a modulator of the Wnt signaling pathway. In mouse palatogenesis, the restricted epithelial expression pattern of Rgs19 was examined in the palatal shelves, where expression of Wnt11 was observed. Based on these specific expression patterns of Rgs19 in the palatal shelves, the present study examined the detailed developmental function of Rgs19 using AS-ODN treatments during in vitro palate organ cultivations as a loss-of-function study. After the knockdown of Rgs19, the morphological changes in the palatal shelves was examined carefully using a computer-aided three dimensional reconstruction method and the altered expression patterns of related signaling molecules were evaluated using genome wide screening methods. RT-qPCR and in situ hybridization methods were also used to confirm these array results. These morphological and molecular examinations suggested that Rgs19 plays important roles in palatal fusion through the degradation of MES via activation of the palatal fusion related and apoptotic related genes. Overall, inhibition of the proliferation related and Wnt responsive genes by Rgs19 are required for proper palatal fusion. © 2012 Elsevier Ireland Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/modoen_US
dc.relation.ispartofMechanisms of Developmenten_US
dc.subjectApoptosisen_US
dc.subjectGenome Wide Screeningen_US
dc.subjectPalatal Fusionen_US
dc.subjectPalatogenesisen_US
dc.subjectRgs19en_US
dc.titleRgs19 regulates mouse palatal fusion by modulating cell proliferation and apoptosis in the MEEen_US
dc.typeArticleen_US
dc.identifier.emailJung, HS: hsjung@yuhs.acen_US
dc.identifier.authorityJung, HS=rp01683en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.mod.2012.07.004en_US
dc.identifier.pmid22841956-
dc.identifier.scopuseid_2-s2.0-84867899114en_US
dc.identifier.isiWOS:000310355900005-
dc.publisher.placeIrelanden_US
dc.identifier.scopusauthoridSohn, WJ=44161404800en_US
dc.identifier.scopusauthoridJi, YR=36672985800en_US
dc.identifier.scopusauthoridKim, HS=35112301400en_US
dc.identifier.scopusauthoridGwon, GJ=52463597100en_US
dc.identifier.scopusauthoridChae, YM=36855355800en_US
dc.identifier.scopusauthoridAn, CH=17134437600en_US
dc.identifier.scopusauthoridPark, Hd=55330107200en_US
dc.identifier.scopusauthoridJung, HS=7403030195en_US
dc.identifier.scopusauthoridRyoo, ZY=16937104900en_US
dc.identifier.scopusauthoridLee, S=7601418915en_US
dc.identifier.scopusauthoridKim, JY=34870132800en_US
dc.identifier.citeulike11799673-
dc.identifier.issnl0925-4773-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats