File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Potential of glucan HBP-A in chondrocytes-alginate hydrogel constructs

TitlePotential of glucan HBP-A in chondrocytes-alginate hydrogel constructs
Authors
Cao, YLPang, JZhan, HSShi, YYAbbah, SALu, WWWang, XWang, SC
KeywordsCartilage
Chondrocyte
Glucan
Hydrogel Constructs
Type Ii Collagen
Issue Date2011
PublisherAcademic Journals. The Journal's web site is located at http://www.academicjournals.org/sre/
Citation
Scientific Research And Essays, 2011, v. 6 n. 6, p. 1364-1370 How to Cite?
AbstractTo explore the biological characteristics of an injectable chondrocytes-alginate hydrogel, constructs in the presence of glucan HBP-A as well as its role in cartilage tissue engineering, chondrocytes were isolated from rabbit knee cartilage and verified by immunocytochemistry for type II collagen. The constructs were embedded with HBP-A and then injected to nude mice subcutaneously. Six weeks after transplantaiton, the specimens were collected for Safranin O staining and transmission electron microscopy (TEM). The mRNA expressions of disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTs-5), aggrecan and type II collegan in constructs were determined by real time PCR. Newly generated cartilaginous tissue was found after transplantation, and the collagen was obvious in the HBP-A group. In accordance with morphological observations, the mRNA expression of type II collagen was also increased significantly, accompanied by decreased expression of ADAMTs-5 (P<0.05) in the HBP-A group compared with the control group. © 2011 Academic Journals.
Persistent Identifierhttp://hdl.handle.net/10722/170175
ISSN
1992-2248
2010 Impact Factor: 0.445
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorCao, YLen_US
dc.contributor.authorPang, Jen_US
dc.contributor.authorZhan, HSen_US
dc.contributor.authorShi, YYen_US
dc.contributor.authorAbbah, SAen_US
dc.contributor.authorLu, WWen_US
dc.contributor.authorWang, Xen_US
dc.contributor.authorWang, SCen_US
dc.date.accessioned2012-10-30T06:05:50Z-
dc.date.available2012-10-30T06:05:50Z-
dc.date.issued2011en_US
dc.identifier.citationScientific Research And Essays, 2011, v. 6 n. 6, p. 1364-1370en_US
dc.identifier.issn1992-2248en_US
dc.identifier.urihttp://hdl.handle.net/10722/170175-
dc.description.abstractTo explore the biological characteristics of an injectable chondrocytes-alginate hydrogel, constructs in the presence of glucan HBP-A as well as its role in cartilage tissue engineering, chondrocytes were isolated from rabbit knee cartilage and verified by immunocytochemistry for type II collagen. The constructs were embedded with HBP-A and then injected to nude mice subcutaneously. Six weeks after transplantaiton, the specimens were collected for Safranin O staining and transmission electron microscopy (TEM). The mRNA expressions of disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTs-5), aggrecan and type II collegan in constructs were determined by real time PCR. Newly generated cartilaginous tissue was found after transplantation, and the collagen was obvious in the HBP-A group. In accordance with morphological observations, the mRNA expression of type II collagen was also increased significantly, accompanied by decreased expression of ADAMTs-5 (P<0.05) in the HBP-A group compared with the control group. © 2011 Academic Journals.en_US
dc.languageengen_US
dc.publisherAcademic Journals. The Journal's web site is located at http://www.academicjournals.org/sre/en_US
dc.relation.ispartofScientific Research and Essaysen_US
dc.subjectCartilageen_US
dc.subjectChondrocyteen_US
dc.subjectGlucanen_US
dc.subjectHydrogel Constructsen_US
dc.subjectType Ii Collagenen_US
dc.titlePotential of glucan HBP-A in chondrocytes-alginate hydrogel constructsen_US
dc.typeArticleen_US
dc.identifier.emailLu, WW:wwlu@hku.hken_US
dc.identifier.authorityLu, WW=rp00411en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-79957643544en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79957643544&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume6en_US
dc.identifier.issue6en_US
dc.identifier.spage1364en_US
dc.identifier.epage1370en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridCao, YL=9036350500en_US
dc.identifier.scopusauthoridPang, J=7201733948en_US
dc.identifier.scopusauthoridZhan, HS=7102409198en_US
dc.identifier.scopusauthoridShi, YY=8275964800en_US
dc.identifier.scopusauthoridAbbah, SA=14032930600en_US
dc.identifier.scopusauthoridLu, WW=7404215221en_US
dc.identifier.scopusauthoridWang, X=54386136200en_US
dc.identifier.scopusauthoridWang, SC=54386338300en_US
dc.identifier.issnl1992-2248-

Export via OAI-PMH Interface in XML Formats

OR

Export to Other Non-XML Formats