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- Publisher Website: 10.1111/j.1476-5381.1988.tb10306.x
- Scopus: eid_2-s2.0-0023867044
- PMID: 2453240
- WOS: WOS:A1988M347400007
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Article: Endothelium-dependent hyperpolarization of canine coronary smooth muscle
Title | Endothelium-dependent hyperpolarization of canine coronary smooth muscle |
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Authors | |
Keywords | acetylcholine cyclooxygenase endothelium‐derived relaxing factor(s) hyperpolarization membrane potential sodium‐potassium pump vascular smooth muscle |
Issue Date | 1988 |
Publisher | John Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1 |
Citation | British Journal Of Pharmacology, 1988, v. 93 n. 3, p. 515-524 How to Cite? |
Abstract | 1. Experiments were designed to determine whether endothelium-dependent relaxing factor(s) released by acetylcholine from the canine femoral artery influences the membrane potential of coronary arterial smooth muscle. 2. The membrane potential was recorded in small canine coronary arteries (internal diameter ≤ 500 μm; without endothelium) by means of intracellular microelectrodes. The organ bath also contained a strip of left descending coronary artery without endothelium in which isometric force was measured to bioassay relaxing factor(s) as well as segments of femoral artery with endothelium, which served as the source of endothelium-derived relaxing factor(s). 3. Acetylcholine induced endothelium-dependent, transient hyperpolarizations and relaxations that were not affected by indomethacin. 4. Inhibition of the sodium-potassium pump by ouabain or potassium-free solution did not inhibit the relaxation to acetylcholine but prevented the corresponding hyperpolarization. 5. Activation of the sodium-potassium pump of the smooth muscle cells by readmission of potassium ions after incubation in potassium-free solution caused relaxation and marked hyperpolarization. 6. These results suggest that endothelium-derived relaxing factor(s) induces hyperpolarization of vascular smooth muscle of the canine coronary artery, possibly by activation of sodium-potassium pumping, but that this effect on the cell membrane may only partially explain endothelium-dependent relaxations evoked by acetylcholine. |
Persistent Identifier | http://hdl.handle.net/10722/170891 |
ISSN | 2023 Impact Factor: 6.8 2023 SCImago Journal Rankings: 2.119 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Feletou, M | en_US |
dc.contributor.author | Vanhoutte, PM | en_US |
dc.date.accessioned | 2012-10-30T06:11:18Z | - |
dc.date.available | 2012-10-30T06:11:18Z | - |
dc.date.issued | 1988 | en_US |
dc.identifier.citation | British Journal Of Pharmacology, 1988, v. 93 n. 3, p. 515-524 | en_US |
dc.identifier.issn | 0007-1188 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/170891 | - |
dc.description.abstract | 1. Experiments were designed to determine whether endothelium-dependent relaxing factor(s) released by acetylcholine from the canine femoral artery influences the membrane potential of coronary arterial smooth muscle. 2. The membrane potential was recorded in small canine coronary arteries (internal diameter ≤ 500 μm; without endothelium) by means of intracellular microelectrodes. The organ bath also contained a strip of left descending coronary artery without endothelium in which isometric force was measured to bioassay relaxing factor(s) as well as segments of femoral artery with endothelium, which served as the source of endothelium-derived relaxing factor(s). 3. Acetylcholine induced endothelium-dependent, transient hyperpolarizations and relaxations that were not affected by indomethacin. 4. Inhibition of the sodium-potassium pump by ouabain or potassium-free solution did not inhibit the relaxation to acetylcholine but prevented the corresponding hyperpolarization. 5. Activation of the sodium-potassium pump of the smooth muscle cells by readmission of potassium ions after incubation in potassium-free solution caused relaxation and marked hyperpolarization. 6. These results suggest that endothelium-derived relaxing factor(s) induces hyperpolarization of vascular smooth muscle of the canine coronary artery, possibly by activation of sodium-potassium pumping, but that this effect on the cell membrane may only partially explain endothelium-dependent relaxations evoked by acetylcholine. | en_US |
dc.language | eng | en_US |
dc.publisher | John Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1 | en_US |
dc.relation.ispartof | British Journal of Pharmacology | en_US |
dc.subject | acetylcholine | - |
dc.subject | cyclooxygenase | - |
dc.subject | endothelium‐derived relaxing factor(s) | - |
dc.subject | hyperpolarization | - |
dc.subject | membrane potential | - |
dc.subject | sodium‐potassium pump | - |
dc.subject | vascular smooth muscle | - |
dc.subject.mesh | Acetylcholine - Pharmacology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Biological Agents - Pharmacology | en_US |
dc.subject.mesh | Coronary Vessels - Drug Effects | en_US |
dc.subject.mesh | Dogs | en_US |
dc.subject.mesh | Ion Channels - Drug Effects | en_US |
dc.subject.mesh | Membrane Potentials - Drug Effects | en_US |
dc.subject.mesh | Muscle, Smooth, Vascular - Drug Effects | en_US |
dc.subject.mesh | Nitric Oxide | en_US |
dc.subject.mesh | Potassium - Metabolism | en_US |
dc.subject.mesh | Sodium - Metabolism | en_US |
dc.subject.mesh | Tetraethylammonium | en_US |
dc.subject.mesh | Tetraethylammonium Compounds - Pharmacology | en_US |
dc.subject.mesh | Vasodilator Agents - Pharmacology | en_US |
dc.title | Endothelium-dependent hyperpolarization of canine coronary smooth muscle | en_US |
dc.type | Article | en_US |
dc.identifier.email | Vanhoutte, PM:vanhoutt@hku.hk | en_US |
dc.identifier.authority | Vanhoutte, PM=rp00238 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1111/j.1476-5381.1988.tb10306.x | - |
dc.identifier.pmid | 2453240 | en_US |
dc.identifier.scopus | eid_2-s2.0-0023867044 | en_US |
dc.identifier.volume | 93 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 515 | en_US |
dc.identifier.epage | 524 | en_US |
dc.identifier.isi | WOS:A1988M347400007 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Feletou, M=7006461826 | en_US |
dc.identifier.scopusauthorid | Vanhoutte, PM=7202304247 | en_US |
dc.identifier.issnl | 0007-1188 | - |