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- Publisher Website: 10.1161/01.CIR.83.2.652
- Scopus: eid_2-s2.0-0026058284
- PMID: 1991383
- WOS: WOS:A1991EW94000031
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Article: Loss of endothelial pertussis toxin-sensitive G protein function in atherosclerotic porcine coronary arteries
Title | Loss of endothelial pertussis toxin-sensitive G protein function in atherosclerotic porcine coronary arteries |
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Authors | |
Keywords | A23187 ADP Atherosclerosis Bradykinin Endothelium-derived relaxing factor G proteins Hypercholesterolemia Pertussis toxin Regenerated endothelium Serotonin Thrombin α2-adrenergic receptors |
Issue Date | 1991 |
Publisher | Lippincott Williams & Wilkins. The Journal's web site is located at http://circ.ahajournals.org |
Citation | Circulation, 1991, v. 83 n. 2, p. 652-660 How to Cite? |
Abstract | Pertussis toxin, an irreversible inhibitor of some G proteins, inhibits endothelium-dependent relaxations to certain agonists in porcine coronary arteries. In the present study, the effects of the toxin were examined on endothelium-dependent and -independent relaxations of hypercholesterolemic and atherosclerotic porcine coronary arteries to assess the functional state of the endothelial pertussis toxin-sensitive G protein. Male Yorkshire pigs were maintained on either a regular diet (control group, n = 7) or a 2% high-cholesterol diet (cholesterol-fed group, n = 7) for 10 weeks. After the initial 2 weeks of maintenance, animals in both groups underwent balloon catheter removal of the endothelium of the left anterior descending or left circumflex coronary arteries. Endothelium-dependent responses were examined in vitro after 10 weeks of maintenance; at this time, a full lining of endothelial cells in both left coronary arteries was confirmed histologically. In arteries with endothelium of the control group (normal responses), pertussis toxin significantly inhibited the endothelium-dependent relaxations to serotonin, UK14304 (a selective α2-adrenergic receptor agonist), and thrombin but not those to ABP, bradykinin, or the calcium ionophore A23187. In previously denuded arteries of the control group (effects of endothelial regeneration alone) or intact arteries of the cholesterol-fed group (effects of hypercholesterolemia alone), the relaxations to serotonin, UK14304, and thrombin were impaired significantly; those relaxations were impaired further in previously denuded arteries of the cholesterol-fed group (effects of atherosclerosis). The inhibitory effects of pertussis toxin were significantly reduced after endothelial regeneration and in hypercholesterolemia and were almost absent in atherosclerosis. Direct relaxations of coronary vascular smooth muscle evoked by nitric oxide or sodium nitroprusside were not significantly affected by either hypercholesterolemia or atherosclerosis. These results indicate that the function of endothelial pertussis toxin-sensitive G protein is impaired in regenerated endothelial cells or hypercholesterolemia and is almost absent in atherosclerosis, accounting in part for the endothelial dysfunction under those pathological conditions. |
Persistent Identifier | http://hdl.handle.net/10722/171028 |
ISSN | 2023 Impact Factor: 35.5 2023 SCImago Journal Rankings: 8.415 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Shimokawa, H | en_US |
dc.contributor.author | Flavahan, NA | en_US |
dc.contributor.author | Vanhoutte, PM | en_US |
dc.date.accessioned | 2012-10-30T06:11:53Z | - |
dc.date.available | 2012-10-30T06:11:53Z | - |
dc.date.issued | 1991 | en_US |
dc.identifier.citation | Circulation, 1991, v. 83 n. 2, p. 652-660 | en_US |
dc.identifier.issn | 0009-7322 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171028 | - |
dc.description.abstract | Pertussis toxin, an irreversible inhibitor of some G proteins, inhibits endothelium-dependent relaxations to certain agonists in porcine coronary arteries. In the present study, the effects of the toxin were examined on endothelium-dependent and -independent relaxations of hypercholesterolemic and atherosclerotic porcine coronary arteries to assess the functional state of the endothelial pertussis toxin-sensitive G protein. Male Yorkshire pigs were maintained on either a regular diet (control group, n = 7) or a 2% high-cholesterol diet (cholesterol-fed group, n = 7) for 10 weeks. After the initial 2 weeks of maintenance, animals in both groups underwent balloon catheter removal of the endothelium of the left anterior descending or left circumflex coronary arteries. Endothelium-dependent responses were examined in vitro after 10 weeks of maintenance; at this time, a full lining of endothelial cells in both left coronary arteries was confirmed histologically. In arteries with endothelium of the control group (normal responses), pertussis toxin significantly inhibited the endothelium-dependent relaxations to serotonin, UK14304 (a selective α2-adrenergic receptor agonist), and thrombin but not those to ABP, bradykinin, or the calcium ionophore A23187. In previously denuded arteries of the control group (effects of endothelial regeneration alone) or intact arteries of the cholesterol-fed group (effects of hypercholesterolemia alone), the relaxations to serotonin, UK14304, and thrombin were impaired significantly; those relaxations were impaired further in previously denuded arteries of the cholesterol-fed group (effects of atherosclerosis). The inhibitory effects of pertussis toxin were significantly reduced after endothelial regeneration and in hypercholesterolemia and were almost absent in atherosclerosis. Direct relaxations of coronary vascular smooth muscle evoked by nitric oxide or sodium nitroprusside were not significantly affected by either hypercholesterolemia or atherosclerosis. These results indicate that the function of endothelial pertussis toxin-sensitive G protein is impaired in regenerated endothelial cells or hypercholesterolemia and is almost absent in atherosclerosis, accounting in part for the endothelial dysfunction under those pathological conditions. | en_US |
dc.language | eng | en_US |
dc.publisher | Lippincott Williams & Wilkins. The Journal's web site is located at http://circ.ahajournals.org | en_US |
dc.relation.ispartof | Circulation | en_US |
dc.subject | A23187 | - |
dc.subject | ADP | - |
dc.subject | Atherosclerosis | - |
dc.subject | Bradykinin | - |
dc.subject | Endothelium-derived relaxing factor | - |
dc.subject | G proteins | - |
dc.subject | Hypercholesterolemia | - |
dc.subject | Pertussis toxin | - |
dc.subject | Regenerated endothelium | - |
dc.subject | Serotonin | - |
dc.subject | Thrombin | - |
dc.subject | α2-adrenergic receptors | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Coronary Artery Disease - Physiopathology | en_US |
dc.subject.mesh | Coronary Vessels - Drug Effects - Physiopathology | en_US |
dc.subject.mesh | Endothelium, Vascular - Drug Effects - Physiopathology | en_US |
dc.subject.mesh | Hypercholesterolemia - Physiopathology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Muscle Contraction - Physiology | en_US |
dc.subject.mesh | Nitric Oxide - Physiology | en_US |
dc.subject.mesh | Pertussis Toxin | en_US |
dc.subject.mesh | Swine | en_US |
dc.subject.mesh | Virulence Factors, Bordetella - Pharmacology | en_US |
dc.title | Loss of endothelial pertussis toxin-sensitive G protein function in atherosclerotic porcine coronary arteries | en_US |
dc.type | Article | en_US |
dc.identifier.email | Vanhoutte, PM:vanhoutt@hku.hk | en_US |
dc.identifier.authority | Vanhoutte, PM=rp00238 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1161/01.CIR.83.2.652 | - |
dc.identifier.pmid | 1991383 | - |
dc.identifier.scopus | eid_2-s2.0-0026058284 | en_US |
dc.identifier.volume | 83 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 652 | en_US |
dc.identifier.epage | 660 | en_US |
dc.identifier.isi | WOS:A1991EW94000031 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Shimokawa, H=16684837100 | en_US |
dc.identifier.scopusauthorid | Flavahan, NA=7006398882 | en_US |
dc.identifier.scopusauthorid | Vanhoutte, PM=7202304247 | en_US |
dc.identifier.issnl | 0009-7322 | - |