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- Scopus: eid_2-s2.0-0027419488
- PMID: 8447456
- WOS: WOS:A1993KN68100017
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Article: Induction of NO production by TNF-α and lipopolysaccharide in porcine coronary arteries without endothelium
Title | Induction of NO production by TNF-α and lipopolysaccharide in porcine coronary arteries without endothelium |
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Authors | |
Keywords | 5-hydroxytryptamine coronary artery L-arginine nitric oxide pig tumor necrosis factor |
Issue Date | 1993 |
Publisher | American Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/ |
Citation | American Journal Of Physiology - Heart And Circulatory Physiology, 1993, v. 264 n. 2 33-2, p. H403-H407 How to Cite? |
Abstract | The effects of tumor necrosis factor-α (TNF-α) and lipopolysaccharide (LPS) were studied in porcine coronary arteries without endothelium. Rings of the artery were incubated in minimum essential medium with TNF-α or LPS for 6 or 24 h. After 6 h of incubation, the rings were suspended in organ chambers filled with physiological salt solution containing indomethacin for the measurement of isometric force. The rings were contracted with prostaglandin F(2α) before the addition of L-arginine. In rings treated with TNF-α or LPS, L-arginine caused a concentration-dependent relaxation that was abolished by N(ω)-nitro-L-arginine [an inhibitor of nitric oxide (NO) synthase]. However, contractions to 5-hydroxytryptamine were not affected by TNF-α and LPS. After 24 h of incubation, TNF and LPS impaired the contractions to 5-hydroxytryptamine and increased the accumulation of nitrite, a stable degradation product of NO. These effects of TNF-α and LPS were blocked by N(ω)-nitro-L-arginine. Cycloheximide (an inhibitor of protein synthesis) attenuated the inhibitory effect of TNF-α and LPS on contractions to 5-hydroxytryptamine. Thus, in the porcine coronary artery without endothelium, TNF-α and LPS can induce an L-arginine-NO pathway. |
Persistent Identifier | http://hdl.handle.net/10722/171104 |
ISSN | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Shibano, T | en_US |
dc.contributor.author | Vanhoutte, PM | en_US |
dc.date.accessioned | 2012-10-30T06:12:12Z | - |
dc.date.available | 2012-10-30T06:12:12Z | - |
dc.date.issued | 1993 | en_US |
dc.identifier.citation | American Journal Of Physiology - Heart And Circulatory Physiology, 1993, v. 264 n. 2 33-2, p. H403-H407 | en_US |
dc.identifier.issn | 0002-9513 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171104 | - |
dc.description.abstract | The effects of tumor necrosis factor-α (TNF-α) and lipopolysaccharide (LPS) were studied in porcine coronary arteries without endothelium. Rings of the artery were incubated in minimum essential medium with TNF-α or LPS for 6 or 24 h. After 6 h of incubation, the rings were suspended in organ chambers filled with physiological salt solution containing indomethacin for the measurement of isometric force. The rings were contracted with prostaglandin F(2α) before the addition of L-arginine. In rings treated with TNF-α or LPS, L-arginine caused a concentration-dependent relaxation that was abolished by N(ω)-nitro-L-arginine [an inhibitor of nitric oxide (NO) synthase]. However, contractions to 5-hydroxytryptamine were not affected by TNF-α and LPS. After 24 h of incubation, TNF and LPS impaired the contractions to 5-hydroxytryptamine and increased the accumulation of nitrite, a stable degradation product of NO. These effects of TNF-α and LPS were blocked by N(ω)-nitro-L-arginine. Cycloheximide (an inhibitor of protein synthesis) attenuated the inhibitory effect of TNF-α and LPS on contractions to 5-hydroxytryptamine. Thus, in the porcine coronary artery without endothelium, TNF-α and LPS can induce an L-arginine-NO pathway. | en_US |
dc.language | eng | en_US |
dc.publisher | American Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/ | en_US |
dc.relation.ispartof | American Journal of Physiology - Heart and Circulatory Physiology | en_US |
dc.subject | 5-hydroxytryptamine | - |
dc.subject | coronary artery | - |
dc.subject | L-arginine | - |
dc.subject | nitric oxide | - |
dc.subject | pig | - |
dc.subject | tumor necrosis factor | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Arginine - Pharmacology | en_US |
dc.subject.mesh | Coronary Vessels - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Endothelium, Vascular - Physiology | en_US |
dc.subject.mesh | Lipopolysaccharides - Pharmacology | en_US |
dc.subject.mesh | Nitric Oxide - Metabolism | en_US |
dc.subject.mesh | Nitrites - Metabolism | en_US |
dc.subject.mesh | Serotonin - Pharmacology | en_US |
dc.subject.mesh | Swine | en_US |
dc.subject.mesh | Time Factors | en_US |
dc.subject.mesh | Tumor Necrosis Factor-Alpha - Pharmacology | en_US |
dc.subject.mesh | Vasodilation | en_US |
dc.title | Induction of NO production by TNF-α and lipopolysaccharide in porcine coronary arteries without endothelium | en_US |
dc.type | Article | en_US |
dc.identifier.email | Vanhoutte, PM:vanhoutt@hku.hk | en_US |
dc.identifier.authority | Vanhoutte, PM=rp00238 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 8447456 | - |
dc.identifier.scopus | eid_2-s2.0-0027419488 | en_US |
dc.identifier.volume | 264 | en_US |
dc.identifier.issue | 2 33-2 | en_US |
dc.identifier.spage | H403 | en_US |
dc.identifier.epage | H407 | en_US |
dc.identifier.isi | WOS:A1993KN68100017 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Shibano, T=7006946465 | en_US |
dc.identifier.scopusauthorid | Vanhoutte, PM=7202304247 | en_US |
dc.identifier.issnl | 0002-9513 | - |