File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1152/ajpheart.91513.2007
- Scopus: eid_2-s2.0-54049123058
- PMID: 18641267
- WOS: WOS:000258949200035
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Stimulation of ecto-5′-nucleotidase in human umbilical vein endothelial cells by lipopolysaccharide
Title | Stimulation of ecto-5′-nucleotidase in human umbilical vein endothelial cells by lipopolysaccharide |
---|---|
Authors | |
Keywords | Adenosine Cytokines Inflammation |
Issue Date | 2008 |
Publisher | American Physiological Society. The Journal's web site is located at http://intl-ajpheart.physiology.org/ |
Citation | American Journal Of Physiology - Heart And Circulatory Physiology, 2008, v. 295 n. 3, p. H1177-H1181 How to Cite? |
Abstract | The involvement of ecto-5′-nucleotidase (E-5′Nu) in the elevation of extracellular adenosine during inflammation is unclear. In the present study, the effect of lipopolysaccharide (LPS), an inflammation inducer, was investigated on E-5′Nu in human umbilical vein endothelial cells (HUVECs). E-5′Nu activity was enhanced after a 24 h exposure to LPS. This effect was dose dependent, with an EC 50 of 1.66 ng/ml. At 10 μM, the phosphatidylinositol 3-kinase (PI3K) inhibitor LY-294002 abolished the LPS-induced E-5′Nu activity. However, at 10 μM, the NF-κB inhibitor ammonium pyrrolidine dithiocarbamate had no effect. LPS upregulated the protein expression but not the messenger RNA expression of E-5′Nu. The inhibition of E-5′Nu by 100 μM α,β-methylene adenosine-5′-diphosphate increased the LPS-induced inflammation, suggesting that E-5′Nu plays a significant role in reducing inflammation, probably through the generation of adenosine. In conclusion, the experiments indicate that LPS upregulates E-5′Nu activity in HUVECs through a PI3K-dependent increase in the abundance of E-5′Nu on cell membranes. Since adenosine is an anti-inflammatory molecule, E-5′Nu upregulation may be crucial in protecting endothelial cells against inflammatory damage. Copyright © 2008 the American Physiological Society. |
Persistent Identifier | http://hdl.handle.net/10722/171372 |
ISSN | 2023 Impact Factor: 4.1 2023 SCImago Journal Rankings: 1.452 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, RWS | en_US |
dc.contributor.author | Man, RYK | en_US |
dc.contributor.author | Vanhoutte, PM | en_US |
dc.contributor.author | Leung, GPH | en_US |
dc.date.accessioned | 2012-10-30T06:13:40Z | - |
dc.date.available | 2012-10-30T06:13:40Z | - |
dc.date.issued | 2008 | en_US |
dc.identifier.citation | American Journal Of Physiology - Heart And Circulatory Physiology, 2008, v. 295 n. 3, p. H1177-H1181 | en_US |
dc.identifier.issn | 0363-6135 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171372 | - |
dc.description.abstract | The involvement of ecto-5′-nucleotidase (E-5′Nu) in the elevation of extracellular adenosine during inflammation is unclear. In the present study, the effect of lipopolysaccharide (LPS), an inflammation inducer, was investigated on E-5′Nu in human umbilical vein endothelial cells (HUVECs). E-5′Nu activity was enhanced after a 24 h exposure to LPS. This effect was dose dependent, with an EC 50 of 1.66 ng/ml. At 10 μM, the phosphatidylinositol 3-kinase (PI3K) inhibitor LY-294002 abolished the LPS-induced E-5′Nu activity. However, at 10 μM, the NF-κB inhibitor ammonium pyrrolidine dithiocarbamate had no effect. LPS upregulated the protein expression but not the messenger RNA expression of E-5′Nu. The inhibition of E-5′Nu by 100 μM α,β-methylene adenosine-5′-diphosphate increased the LPS-induced inflammation, suggesting that E-5′Nu plays a significant role in reducing inflammation, probably through the generation of adenosine. In conclusion, the experiments indicate that LPS upregulates E-5′Nu activity in HUVECs through a PI3K-dependent increase in the abundance of E-5′Nu on cell membranes. Since adenosine is an anti-inflammatory molecule, E-5′Nu upregulation may be crucial in protecting endothelial cells against inflammatory damage. Copyright © 2008 the American Physiological Society. | en_US |
dc.language | eng | en_US |
dc.publisher | American Physiological Society. The Journal's web site is located at http://intl-ajpheart.physiology.org/ | en_US |
dc.relation.ispartof | American Journal of Physiology - Heart and Circulatory Physiology | en_US |
dc.subject | Adenosine | en_US |
dc.subject | Cytokines | en_US |
dc.subject | Inflammation | en_US |
dc.title | Stimulation of ecto-5′-nucleotidase in human umbilical vein endothelial cells by lipopolysaccharide | en_US |
dc.type | Article | en_US |
dc.identifier.email | Man, RYK:rykman@hkucc.hku.hk | en_US |
dc.identifier.email | Vanhoutte, PM:vanhoutt@hku.hk | en_US |
dc.identifier.email | Leung, GPH:gphleung@hkucc.hku.hk | en_US |
dc.identifier.authority | Man, RYK=rp00236 | en_US |
dc.identifier.authority | Vanhoutte, PM=rp00238 | en_US |
dc.identifier.authority | Leung, GPH=rp00234 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1152/ajpheart.91513.2007 | en_US |
dc.identifier.pmid | 18641267 | - |
dc.identifier.scopus | eid_2-s2.0-54049123058 | en_US |
dc.identifier.hkuros | 147882 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-54049123058&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 295 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | H1177 | en_US |
dc.identifier.epage | H1181 | en_US |
dc.identifier.eissn | 1522-1539 | - |
dc.identifier.isi | WOS:000258949200035 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Li, RWS=7404722884 | en_US |
dc.identifier.scopusauthorid | Man, RYK=7004986435 | en_US |
dc.identifier.scopusauthorid | Vanhoutte, PM=7202304247 | en_US |
dc.identifier.scopusauthorid | Leung, GPH=35963668200 | en_US |
dc.identifier.issnl | 0363-6135 | - |