File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Scopus: eid_2-s2.0-0021342999
- PMID: 6325412
- WOS: WOS:A1984SP38500042
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Sodium and proton transport in flagella isolated from sea urchin spermatozoa
Title | Sodium and proton transport in flagella isolated from sea urchin spermatozoa |
---|---|
Authors | |
Issue Date | 1984 |
Publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ |
Citation | Journal Of Biological Chemistry, 1984, v. 259 n. 8, p. 4957-4963 How to Cite? |
Abstract | A method was developed to isolate flagella with intact membranes from sea urchin sperm to further study the mechanism of the Na+-dependent H+ extrusion which increases the intracellular pH and triggers motility. Based on cytochrome c oxidase activity and cell count, the flagellar preparation contained at most 0.05% of the sperm heads present in the initial homogenate. The isolated flagella retained a Na+-dependent H+ extrusion mechanism which, like that of intact sperm, was inhibitable by high external potassium and with an ionic selectivity in the order of Na+ > Li+ >> Rb+ ≃ Cs+ (essentially ineffective). Two methods were employed to measure the change in intraflagellar pH (pH(i)): the accumulation of [14C]methylamine and a spectrofluorimetric technique using acridine orange. Flagella isolated from sperm suspended in Na+-free seawater (pH 7.90) maintained a pH(i) of 6.72 ± 0.05 which increased by 0.5 to 0.6 pH unit following a Na+ (10 mM)-dependent release of H+ (114 ± 18 nmol/mg) and an uptake of 22Na+ (134 ± 36 nmol/mg). Both ionic movements were inhibitable by high external K+, suggesting a coupled exchange mechanism. This study provides direct evidence for the alkalinization of the flagellar pH under a condition compatible with motility initiation and shows that the isolated flagella is a useful model system for studying its mechanism. |
Persistent Identifier | http://hdl.handle.net/10722/171490 |
ISSN | 2020 Impact Factor: 5.157 2023 SCImago Journal Rankings: 1.766 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, HC | en_US |
dc.date.accessioned | 2012-10-30T06:15:24Z | - |
dc.date.available | 2012-10-30T06:15:24Z | - |
dc.date.issued | 1984 | en_US |
dc.identifier.citation | Journal Of Biological Chemistry, 1984, v. 259 n. 8, p. 4957-4963 | en_US |
dc.identifier.issn | 0021-9258 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171490 | - |
dc.description.abstract | A method was developed to isolate flagella with intact membranes from sea urchin sperm to further study the mechanism of the Na+-dependent H+ extrusion which increases the intracellular pH and triggers motility. Based on cytochrome c oxidase activity and cell count, the flagellar preparation contained at most 0.05% of the sperm heads present in the initial homogenate. The isolated flagella retained a Na+-dependent H+ extrusion mechanism which, like that of intact sperm, was inhibitable by high external potassium and with an ionic selectivity in the order of Na+ > Li+ >> Rb+ ≃ Cs+ (essentially ineffective). Two methods were employed to measure the change in intraflagellar pH (pH(i)): the accumulation of [14C]methylamine and a spectrofluorimetric technique using acridine orange. Flagella isolated from sperm suspended in Na+-free seawater (pH 7.90) maintained a pH(i) of 6.72 ± 0.05 which increased by 0.5 to 0.6 pH unit following a Na+ (10 mM)-dependent release of H+ (114 ± 18 nmol/mg) and an uptake of 22Na+ (134 ± 36 nmol/mg). Both ionic movements were inhibitable by high external K+, suggesting a coupled exchange mechanism. This study provides direct evidence for the alkalinization of the flagellar pH under a condition compatible with motility initiation and shows that the isolated flagella is a useful model system for studying its mechanism. | en_US |
dc.language | eng | en_US |
dc.publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ | en_US |
dc.relation.ispartof | Journal of Biological Chemistry | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Carrier Proteins - Metabolism | en_US |
dc.subject.mesh | Cations, Monovalent | en_US |
dc.subject.mesh | Electron Transport Complex Iv - Metabolism | en_US |
dc.subject.mesh | Flagella - Metabolism - Ultrastructure | en_US |
dc.subject.mesh | Hydrogen-Ion Concentration | en_US |
dc.subject.mesh | Kinetics | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Sea Urchins | en_US |
dc.subject.mesh | Sodium - Metabolism | en_US |
dc.subject.mesh | Sodium-Hydrogen Antiporter | en_US |
dc.subject.mesh | Spermatozoa - Metabolism | en_US |
dc.title | Sodium and proton transport in flagella isolated from sea urchin spermatozoa | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lee, HC:leehc@hku.hk | en_US |
dc.identifier.authority | Lee, HC=rp00545 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 6325412 | - |
dc.identifier.scopus | eid_2-s2.0-0021342999 | en_US |
dc.identifier.volume | 259 | en_US |
dc.identifier.issue | 8 | en_US |
dc.identifier.spage | 4957 | en_US |
dc.identifier.epage | 4963 | en_US |
dc.identifier.isi | WOS:A1984SP38500042 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Lee, HC=26642959100 | en_US |
dc.identifier.issnl | 0021-9258 | - |