File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Sodium and proton transport in flagella isolated from sea urchin spermatozoa

TitleSodium and proton transport in flagella isolated from sea urchin spermatozoa
Authors
Issue Date1984
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 1984, v. 259 n. 8, p. 4957-4963 How to Cite?
AbstractA method was developed to isolate flagella with intact membranes from sea urchin sperm to further study the mechanism of the Na+-dependent H+ extrusion which increases the intracellular pH and triggers motility. Based on cytochrome c oxidase activity and cell count, the flagellar preparation contained at most 0.05% of the sperm heads present in the initial homogenate. The isolated flagella retained a Na+-dependent H+ extrusion mechanism which, like that of intact sperm, was inhibitable by high external potassium and with an ionic selectivity in the order of Na+ > Li+ >> Rb+ ≃ Cs+ (essentially ineffective). Two methods were employed to measure the change in intraflagellar pH (pH(i)): the accumulation of [14C]methylamine and a spectrofluorimetric technique using acridine orange. Flagella isolated from sperm suspended in Na+-free seawater (pH 7.90) maintained a pH(i) of 6.72 ± 0.05 which increased by 0.5 to 0.6 pH unit following a Na+ (10 mM)-dependent release of H+ (114 ± 18 nmol/mg) and an uptake of 22Na+ (134 ± 36 nmol/mg). Both ionic movements were inhibitable by high external K+, suggesting a coupled exchange mechanism. This study provides direct evidence for the alkalinization of the flagellar pH under a condition compatible with motility initiation and shows that the isolated flagella is a useful model system for studying its mechanism.
Persistent Identifierhttp://hdl.handle.net/10722/171490
ISSN
2020 Impact Factor: 5.157
2023 SCImago Journal Rankings: 1.766
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLee, HCen_US
dc.date.accessioned2012-10-30T06:15:24Z-
dc.date.available2012-10-30T06:15:24Z-
dc.date.issued1984en_US
dc.identifier.citationJournal Of Biological Chemistry, 1984, v. 259 n. 8, p. 4957-4963en_US
dc.identifier.issn0021-9258en_US
dc.identifier.urihttp://hdl.handle.net/10722/171490-
dc.description.abstractA method was developed to isolate flagella with intact membranes from sea urchin sperm to further study the mechanism of the Na+-dependent H+ extrusion which increases the intracellular pH and triggers motility. Based on cytochrome c oxidase activity and cell count, the flagellar preparation contained at most 0.05% of the sperm heads present in the initial homogenate. The isolated flagella retained a Na+-dependent H+ extrusion mechanism which, like that of intact sperm, was inhibitable by high external potassium and with an ionic selectivity in the order of Na+ > Li+ >> Rb+ ≃ Cs+ (essentially ineffective). Two methods were employed to measure the change in intraflagellar pH (pH(i)): the accumulation of [14C]methylamine and a spectrofluorimetric technique using acridine orange. Flagella isolated from sperm suspended in Na+-free seawater (pH 7.90) maintained a pH(i) of 6.72 ± 0.05 which increased by 0.5 to 0.6 pH unit following a Na+ (10 mM)-dependent release of H+ (114 ± 18 nmol/mg) and an uptake of 22Na+ (134 ± 36 nmol/mg). Both ionic movements were inhibitable by high external K+, suggesting a coupled exchange mechanism. This study provides direct evidence for the alkalinization of the flagellar pH under a condition compatible with motility initiation and shows that the isolated flagella is a useful model system for studying its mechanism.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCarrier Proteins - Metabolismen_US
dc.subject.meshCations, Monovalenten_US
dc.subject.meshElectron Transport Complex Iv - Metabolismen_US
dc.subject.meshFlagella - Metabolism - Ultrastructureen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshKineticsen_US
dc.subject.meshMaleen_US
dc.subject.meshSea Urchinsen_US
dc.subject.meshSodium - Metabolismen_US
dc.subject.meshSodium-Hydrogen Antiporteren_US
dc.subject.meshSpermatozoa - Metabolismen_US
dc.titleSodium and proton transport in flagella isolated from sea urchin spermatozoaen_US
dc.typeArticleen_US
dc.identifier.emailLee, HC:leehc@hku.hken_US
dc.identifier.authorityLee, HC=rp00545en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid6325412-
dc.identifier.scopuseid_2-s2.0-0021342999en_US
dc.identifier.volume259en_US
dc.identifier.issue8en_US
dc.identifier.spage4957en_US
dc.identifier.epage4963en_US
dc.identifier.isiWOS:A1984SP38500042-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLee, HC=26642959100en_US
dc.identifier.issnl0021-9258-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats