File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Scopus: eid_2-s2.0-0024539836
- PMID: 2538075
- WOS: WOS:A1989T693800063
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Proton permeability and lipid dynamics of gastric and duodenal apical membrane vesicles
Title | Proton permeability and lipid dynamics of gastric and duodenal apical membrane vesicles |
---|---|
Authors | |
Issue Date | 1989 |
Publisher | American Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/ |
Citation | American Journal Of Physiology - Gastrointestinal And Liver Physiology, 1989, v. 256 n. 3, p. 19/3 How to Cite? |
Abstract | The passive proton permeability (P(net)) of apical membrane vesicles from rabbit parietal cells (gastric) and duodenal and renal cortical brush-border membranes (BBM) was determined by acridine orange fluorescence quenching. Values of P(net) were found to be gastric (4 x 10-4 cm/s) < duodenal (10-3 cm/s) << renal (10-2 cm/s). Arrhenius plots of the temperature profile of proton permeation of gastric vesicles was linear, whereas that of duodenal BBM displayed a discontinuity at 30-33°C. Alcohols (octyl, benzyl, ethyl) increased P(net) in a concentration-dependent manner, with efficacy related to their oil-water partition coefficients. In a parallel series of experiments, structural parameters of the vesicle membrane lipids (fluidity) were monitored from both the steady-state and time-resolved fluorescence anisotropy of diphenylhexatriene. Fluidity of the membranes was unrelated to P(net) (renal ≃ duodenal < gastric). Gastric vesicles demonstrated a linear Arrhenius plot of temperature dependence for fluidity, whereas duodenal BBM demonstrated a discontinuity. Membrane fluidity of gastric and duodenal vesicles was increased by alcohols, with the same potency as for P(net), and these two variables were significantly correlated after perturbation with alcohols. Thus the fluidity of the lipid bilayer is not the major determinant of P(net), but alteration of its structural parameters, as reflected by fluidity, produces parallel changes in P(net). |
Persistent Identifier | http://hdl.handle.net/10722/171537 |
ISSN | |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wilkes, JM | en_US |
dc.contributor.author | Ballard, HJ | en_US |
dc.contributor.author | Dryden, DTF | en_US |
dc.contributor.author | Hirst, BH | en_US |
dc.date.accessioned | 2012-10-30T06:15:35Z | - |
dc.date.available | 2012-10-30T06:15:35Z | - |
dc.date.issued | 1989 | en_US |
dc.identifier.citation | American Journal Of Physiology - Gastrointestinal And Liver Physiology, 1989, v. 256 n. 3, p. 19/3 | en_US |
dc.identifier.issn | 0002-9513 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171537 | - |
dc.description.abstract | The passive proton permeability (P(net)) of apical membrane vesicles from rabbit parietal cells (gastric) and duodenal and renal cortical brush-border membranes (BBM) was determined by acridine orange fluorescence quenching. Values of P(net) were found to be gastric (4 x 10-4 cm/s) < duodenal (10-3 cm/s) << renal (10-2 cm/s). Arrhenius plots of the temperature profile of proton permeation of gastric vesicles was linear, whereas that of duodenal BBM displayed a discontinuity at 30-33°C. Alcohols (octyl, benzyl, ethyl) increased P(net) in a concentration-dependent manner, with efficacy related to their oil-water partition coefficients. In a parallel series of experiments, structural parameters of the vesicle membrane lipids (fluidity) were monitored from both the steady-state and time-resolved fluorescence anisotropy of diphenylhexatriene. Fluidity of the membranes was unrelated to P(net) (renal ≃ duodenal < gastric). Gastric vesicles demonstrated a linear Arrhenius plot of temperature dependence for fluidity, whereas duodenal BBM demonstrated a discontinuity. Membrane fluidity of gastric and duodenal vesicles was increased by alcohols, with the same potency as for P(net), and these two variables were significantly correlated after perturbation with alcohols. Thus the fluidity of the lipid bilayer is not the major determinant of P(net), but alteration of its structural parameters, as reflected by fluidity, produces parallel changes in P(net). | en_US |
dc.language | eng | en_US |
dc.publisher | American Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/ | en_US |
dc.relation.ispartof | American Journal of Physiology - Gastrointestinal and Liver Physiology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Cell Membrane - Physiology | en_US |
dc.subject.mesh | Cell Membrane Permeability | en_US |
dc.subject.mesh | Duodenum - Physiology | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Gastric Fundus - Physiology | en_US |
dc.subject.mesh | Gastric Mucosa - Physiology | en_US |
dc.subject.mesh | Hydrogen-Ion Concentration | en_US |
dc.subject.mesh | Intestinal Mucosa - Physiology | en_US |
dc.subject.mesh | Kidney Cortex - Physiology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Membrane Fluidity | en_US |
dc.subject.mesh | Membrane Lipids - Physiology | en_US |
dc.subject.mesh | Microvilli - Physiology | en_US |
dc.subject.mesh | Mucous Membrane - Physiology | en_US |
dc.subject.mesh | Parietal Cells, Gastric - Physiology | en_US |
dc.subject.mesh | Protons | en_US |
dc.subject.mesh | Rabbits | en_US |
dc.subject.mesh | Thermodynamics | en_US |
dc.title | Proton permeability and lipid dynamics of gastric and duodenal apical membrane vesicles | en_US |
dc.type | Article | en_US |
dc.identifier.email | Ballard, HJ:ballard@hkucc.hku.hk | en_US |
dc.identifier.authority | Ballard, HJ=rp00367 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 2538075 | - |
dc.identifier.scopus | eid_2-s2.0-0024539836 | en_US |
dc.identifier.volume | 256 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 19/3 | en_US |
dc.identifier.isi | WOS:A1989T693800063 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Wilkes, JM=7102513233 | en_US |
dc.identifier.scopusauthorid | Ballard, HJ=7005286310 | en_US |
dc.identifier.scopusauthorid | Dryden, DTF=7004366661 | en_US |
dc.identifier.scopusauthorid | Hirst, BH=7005256376 | en_US |
dc.identifier.issnl | 0002-9513 | - |