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- Scopus: eid_2-s2.0-0025990128
- PMID: 1716053
- WOS: WOS:A1991GE63700015
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Article: Acetylcholine Ca2+ stores refilling directly involves a dihydropyridine-sensitive channel in dog trachea
Title | Acetylcholine Ca2+ stores refilling directly involves a dihydropyridine-sensitive channel in dog trachea |
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Authors | |
Keywords | Calcium store Cyclopiazonic acid Ryanodine |
Issue Date | 1991 |
Publisher | American Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/ |
Citation | American Journal Of Physiology - Cell Physiology, 1991, v. 261 n. 3 30-3, p. C497-C505 How to Cite? |
Abstract | Repetitive stimulation of the smooth muscle with acetylcholine (ACh) in the continuous presence of nifedipine resulted in a progressive decrease in the developed tension. This was associated with a decrease in the content of the agonist-sensitive intracellular Ca2+ stores. Agonist-sensitive internal Ca2+ stores appeared to be readily depleted by successive or prolonged agonist stimulation in Ca2+-free medium. The refilling of the empty stores when the muscle is at rest required extracellular Ca2+, was decreased by nifedipine, and was increased by BAY K 8644 and by increased external Ca2+ concentration. Refilling of stores during ACh stimulation in Ca2+-containing medium was decreased by nifedipine and by cyclopiazonic acid (CPA), an inhibitor of the sarcoplasmic reticulum (SR) Ca2+ pump, and was potentiated by BAY K 8644. BAY K 8644 reversed the inhibitory effect of CPA on stores Ca2+ refilling. Ryanodine in normal Krebs increased muscle resting tension, an effect not observed in Ca2+-free medium, blocked by nifedipine and enhanced by BAY K 8644. We propose that the refilling of ACh-sensitive internal Ca2+ stores involves two distinct pathways, one dependent on the uptake of cytosolic Ca2+ via a CPA-sensitive SR Ca2+-adenosinetriphosphatase, and the other pathway dependent on extracellular Ca2+ influx via a dihydropyridine-sensitive Ca2+ channel and is CPA insensitive. The refilling pathway between plasmalemma and SR may involve a plasmalemma L-type Ca2+ channel (dihydropyridine sensitive) and the SR Ca2+ release channel (ryanodine sensitive). |
Persistent Identifier | http://hdl.handle.net/10722/171557 |
ISSN | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Bourreau, JP | en_US |
dc.contributor.author | Abela, AP | en_US |
dc.contributor.author | Kwan, CY | en_US |
dc.contributor.author | Daniel, EE | en_US |
dc.date.accessioned | 2012-10-30T06:15:40Z | - |
dc.date.available | 2012-10-30T06:15:40Z | - |
dc.date.issued | 1991 | en_US |
dc.identifier.citation | American Journal Of Physiology - Cell Physiology, 1991, v. 261 n. 3 30-3, p. C497-C505 | en_US |
dc.identifier.issn | 0002-9513 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171557 | - |
dc.description.abstract | Repetitive stimulation of the smooth muscle with acetylcholine (ACh) in the continuous presence of nifedipine resulted in a progressive decrease in the developed tension. This was associated with a decrease in the content of the agonist-sensitive intracellular Ca2+ stores. Agonist-sensitive internal Ca2+ stores appeared to be readily depleted by successive or prolonged agonist stimulation in Ca2+-free medium. The refilling of the empty stores when the muscle is at rest required extracellular Ca2+, was decreased by nifedipine, and was increased by BAY K 8644 and by increased external Ca2+ concentration. Refilling of stores during ACh stimulation in Ca2+-containing medium was decreased by nifedipine and by cyclopiazonic acid (CPA), an inhibitor of the sarcoplasmic reticulum (SR) Ca2+ pump, and was potentiated by BAY K 8644. BAY K 8644 reversed the inhibitory effect of CPA on stores Ca2+ refilling. Ryanodine in normal Krebs increased muscle resting tension, an effect not observed in Ca2+-free medium, blocked by nifedipine and enhanced by BAY K 8644. We propose that the refilling of ACh-sensitive internal Ca2+ stores involves two distinct pathways, one dependent on the uptake of cytosolic Ca2+ via a CPA-sensitive SR Ca2+-adenosinetriphosphatase, and the other pathway dependent on extracellular Ca2+ influx via a dihydropyridine-sensitive Ca2+ channel and is CPA insensitive. The refilling pathway between plasmalemma and SR may involve a plasmalemma L-type Ca2+ channel (dihydropyridine sensitive) and the SR Ca2+ release channel (ryanodine sensitive). | en_US |
dc.language | eng | en_US |
dc.publisher | American Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/ | en_US |
dc.relation.ispartof | American Journal of Physiology - Cell Physiology | en_US |
dc.subject | Calcium store | - |
dc.subject | Cyclopiazonic acid | - |
dc.subject | Ryanodine | - |
dc.subject.mesh | 3-Pyridinecarboxylic Acid, 1,4-Dihydro-2,6-Dimethyl-5-Nitro-4-(2-(Trifluoromethyl)Phenyl)-, Methyl Ester - Pharmacology | en_US |
dc.subject.mesh | Acetylcholine - Pharmacology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Calcium - Metabolism - Pharmacology | en_US |
dc.subject.mesh | Calcium Channels - Drug Effects - Physiology | en_US |
dc.subject.mesh | Dogs | en_US |
dc.subject.mesh | Egtazic Acid - Pharmacology | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Kinetics | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Membrane Potentials - Drug Effects | en_US |
dc.subject.mesh | Muscle Contraction - Drug Effects | en_US |
dc.subject.mesh | Muscle, Smooth - Drug Effects - Physiology | en_US |
dc.subject.mesh | Nifedipine - Pharmacology | en_US |
dc.subject.mesh | Ryanodine - Pharmacology | en_US |
dc.subject.mesh | Trachea - Drug Effects - Physiology | en_US |
dc.title | Acetylcholine Ca2+ stores refilling directly involves a dihydropyridine-sensitive channel in dog trachea | en_US |
dc.type | Article | en_US |
dc.identifier.email | Bourreau, JP:bourreau@hkucc.hku.hk | en_US |
dc.identifier.authority | Bourreau, JP=rp00389 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 1716053 | - |
dc.identifier.scopus | eid_2-s2.0-0025990128 | en_US |
dc.identifier.volume | 261 | en_US |
dc.identifier.issue | 3 30-3 | en_US |
dc.identifier.spage | C497 | en_US |
dc.identifier.epage | C505 | en_US |
dc.identifier.isi | WOS:A1991GE63700015 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Bourreau, JP=7003927886 | en_US |
dc.identifier.scopusauthorid | Abela, AP=6602668587 | en_US |
dc.identifier.scopusauthorid | Kwan, CY=7201421224 | en_US |
dc.identifier.scopusauthorid | Daniel, EE=35474017600 | en_US |
dc.identifier.issnl | 0002-9513 | - |