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- Publisher Website: 10.1113/jphysiol.1991.sp018416
- Scopus: eid_2-s2.0-0026090324
- PMID: 1841964
- WOS: WOS:A1991EW69400006
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Article: The influence of lactic acid on adenosine release from skeletal muscle in anaesthetized dogs
Title | The influence of lactic acid on adenosine release from skeletal muscle in anaesthetized dogs |
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Authors | |
Issue Date | 1991 |
Publisher | Wiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3751 |
Citation | Journal Of Physiology, 1991, v. 433, p. 95-108 How to Cite? |
Abstract | In anaesthetized and artificially ventilated dogs, a gracilis muscle was vascularly isolated and perfused at a constant flow rate of 11.9 ± 2.2 ml min-1 100 g-1 (mean ± S.E.M., n = 16; equivalent to 170.2 ± 21.3% of its resting free flow). Stimulation (3 Hz) of the obturator nerve produced twitch contractions of the gracilis muscle, reduced venous pH from 7.366 ± 0.027 to 7.250 ± 0.031 (n = 5), increased oxygen consumption from 0.62 ± 0.24 to 2.76 ± 0.46 ml min-1 100 g-1 (n = 5) and increased adenosine release from -0.40 ± 0.14 (net uptake) to 1.36 ± 0.50 nmol min-1 100 g-1 (n = 8). Infusion of lactic acid (4.2 mM) into the artery reduced venous pH to 7.281 ± 0.026 (n = 5) and increased adenosine release to 0.96 ± 0.40 nmol min-1 100 g-1 (n = 8), but did not significantly alter oxygen consumption (0.80 ± 0.19 ml min-1 100 g-1; n = 5). Stimulation (3 Hz) in the presence of lactic acid infusion produced no further significant changes in venous pH or adenosine release, but increased oxygen consumption to 2.53 ± 0.37 ml min-1 100 g-1 (n = 5). Infusion of a range of lactic acid concentrations (≥ 1.83 mM) produced dose-dependent increases in adenosine release. The maximum lactic acid concentration tested (5.95 mM) reduced venous pH to 7.249 ± 0.023 (n = 5) and increased adenosine release to 2.64 ± 1.26 nmol min-1 100 g-1 (n = 6). A strong correlation existed between the adenosine release and the venous pH (r = -0.92); points obtained during muscle stimulation and/or lactic acid infusion fell on a single correlation line. The vasoactivity of adenosine administered by close-arterial injection was unaltered by infusion of either lactic acid (7.2 mM) or saline. These results suggest that the release of adenosine from skeletal muscle can be induced by a decrease in pH (probably at an intracellular site), and that this mechanism may contribute to the release of adenosine during muscle contractions. |
Persistent Identifier | http://hdl.handle.net/10722/171560 |
ISSN | 2023 Impact Factor: 4.7 2023 SCImago Journal Rankings: 1.708 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Ballard, HJ | en_US |
dc.date.accessioned | 2012-10-30T06:15:41Z | - |
dc.date.available | 2012-10-30T06:15:41Z | - |
dc.date.issued | 1991 | en_US |
dc.identifier.citation | Journal Of Physiology, 1991, v. 433, p. 95-108 | en_US |
dc.identifier.issn | 0022-3751 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171560 | - |
dc.description.abstract | In anaesthetized and artificially ventilated dogs, a gracilis muscle was vascularly isolated and perfused at a constant flow rate of 11.9 ± 2.2 ml min-1 100 g-1 (mean ± S.E.M., n = 16; equivalent to 170.2 ± 21.3% of its resting free flow). Stimulation (3 Hz) of the obturator nerve produced twitch contractions of the gracilis muscle, reduced venous pH from 7.366 ± 0.027 to 7.250 ± 0.031 (n = 5), increased oxygen consumption from 0.62 ± 0.24 to 2.76 ± 0.46 ml min-1 100 g-1 (n = 5) and increased adenosine release from -0.40 ± 0.14 (net uptake) to 1.36 ± 0.50 nmol min-1 100 g-1 (n = 8). Infusion of lactic acid (4.2 mM) into the artery reduced venous pH to 7.281 ± 0.026 (n = 5) and increased adenosine release to 0.96 ± 0.40 nmol min-1 100 g-1 (n = 8), but did not significantly alter oxygen consumption (0.80 ± 0.19 ml min-1 100 g-1; n = 5). Stimulation (3 Hz) in the presence of lactic acid infusion produced no further significant changes in venous pH or adenosine release, but increased oxygen consumption to 2.53 ± 0.37 ml min-1 100 g-1 (n = 5). Infusion of a range of lactic acid concentrations (≥ 1.83 mM) produced dose-dependent increases in adenosine release. The maximum lactic acid concentration tested (5.95 mM) reduced venous pH to 7.249 ± 0.023 (n = 5) and increased adenosine release to 2.64 ± 1.26 nmol min-1 100 g-1 (n = 6). A strong correlation existed between the adenosine release and the venous pH (r = -0.92); points obtained during muscle stimulation and/or lactic acid infusion fell on a single correlation line. The vasoactivity of adenosine administered by close-arterial injection was unaltered by infusion of either lactic acid (7.2 mM) or saline. These results suggest that the release of adenosine from skeletal muscle can be induced by a decrease in pH (probably at an intracellular site), and that this mechanism may contribute to the release of adenosine during muscle contractions. | en_US |
dc.language | eng | en_US |
dc.publisher | Wiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3751 | en_US |
dc.relation.ispartof | Journal of Physiology | en_US |
dc.subject.mesh | Adenosine - Blood - Secretion | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Dogs | en_US |
dc.subject.mesh | Dose-Response Relationship, Drug | en_US |
dc.subject.mesh | Electric Stimulation | en_US |
dc.subject.mesh | Gases - Blood | en_US |
dc.subject.mesh | Hemodynamics - Drug Effects | en_US |
dc.subject.mesh | Hydrogen-Ion Concentration | en_US |
dc.subject.mesh | Infusions, Intra-Arterial | en_US |
dc.subject.mesh | Lactates - Administration & Dosage - Pharmacology | en_US |
dc.subject.mesh | Lactic Acid | en_US |
dc.subject.mesh | Muscle Contraction - Physiology | en_US |
dc.subject.mesh | Muscles - Drug Effects - Physiology - Secretion | en_US |
dc.title | The influence of lactic acid on adenosine release from skeletal muscle in anaesthetized dogs | en_US |
dc.type | Article | en_US |
dc.identifier.email | Ballard, HJ:ballard@hkucc.hku.hk | en_US |
dc.identifier.authority | Ballard, HJ=rp00367 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1113/jphysiol.1991.sp018416 | - |
dc.identifier.pmid | 1841964 | - |
dc.identifier.scopus | eid_2-s2.0-0026090324 | en_US |
dc.identifier.volume | 433 | en_US |
dc.identifier.spage | 95 | en_US |
dc.identifier.epage | 108 | en_US |
dc.identifier.isi | WOS:A1991EW69400006 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Ballard, HJ=7005286310 | en_US |
dc.identifier.issnl | 0022-3751 | - |