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- Publisher Website: 10.1016/S0024-3205(98)00190-8
- Scopus: eid_2-s2.0-0031637408
- PMID: 9627094
- WOS: WOS:000073527200009
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Article: Effect of melatonin on chloride secretion by human colonic T84 cells
Title | Effect of melatonin on chloride secretion by human colonic T84 cells |
---|---|
Authors | |
Keywords | Cl - Secretion Melatonin T 84 Cells |
Issue Date | 1998 |
Publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie |
Citation | Life Sciences, 1998, v. 62 n. 23, p. 2151-2158 How to Cite? |
Abstract | The effect of melatonin on human colonic T84 cells was studied using the short-circuit current (I(SC)) technique. Basolateral, as well as apical, addition of melatonin stimulated I(SC) in a concentration-dependent manner (EC 50 at about 100 μM). The I(SC) response to melatonin was nearly abolished when external Cl - was removed. The increase in I(SC) was also blocked by apical addition of two Cl - channel blockers, 4,4'-diisothiocyanatostibene-2,2'-disulfonic acid (DIDS) and diphenylamine 2-carboxylic acid (DPC), indicating that melatonin stimulated Cl - secretion. The effect of different melatonin analogs was compared and the order of potency appeased to be 2-iodomelatonin > melatonin > 6-hydroxymelatonin, indicating the involvement of melatonin receptors. However, inhibitors for G(i)-protein, adenylate cyclase or phospholipase C were found to be ineffective in inhibiting the melatonin-induced I(SC). Pretreatment of the cells with melatonin was also found to exert little effect on subsequent forskolin- or VIP-induced I(SC), further excluding its interaction with adenylate cyclase. Our data suggest that melatonin may play a role in regulating colonic Cl - secretion via melatonin receptors; however, the signal transduction pathway(s) involved remains to be elucidated. |
Persistent Identifier | http://hdl.handle.net/10722/171646 |
ISSN | 2021 Impact Factor: 6.780 2020 SCImago Journal Rankings: 1.131 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chan, HC | en_US |
dc.contributor.author | Lui, KM | en_US |
dc.contributor.author | Wong, WS | en_US |
dc.contributor.author | Poon, AMS | en_US |
dc.date.accessioned | 2012-10-30T06:16:08Z | - |
dc.date.available | 2012-10-30T06:16:08Z | - |
dc.date.issued | 1998 | en_US |
dc.identifier.citation | Life Sciences, 1998, v. 62 n. 23, p. 2151-2158 | en_US |
dc.identifier.issn | 0024-3205 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171646 | - |
dc.description.abstract | The effect of melatonin on human colonic T84 cells was studied using the short-circuit current (I(SC)) technique. Basolateral, as well as apical, addition of melatonin stimulated I(SC) in a concentration-dependent manner (EC 50 at about 100 μM). The I(SC) response to melatonin was nearly abolished when external Cl - was removed. The increase in I(SC) was also blocked by apical addition of two Cl - channel blockers, 4,4'-diisothiocyanatostibene-2,2'-disulfonic acid (DIDS) and diphenylamine 2-carboxylic acid (DPC), indicating that melatonin stimulated Cl - secretion. The effect of different melatonin analogs was compared and the order of potency appeased to be 2-iodomelatonin > melatonin > 6-hydroxymelatonin, indicating the involvement of melatonin receptors. However, inhibitors for G(i)-protein, adenylate cyclase or phospholipase C were found to be ineffective in inhibiting the melatonin-induced I(SC). Pretreatment of the cells with melatonin was also found to exert little effect on subsequent forskolin- or VIP-induced I(SC), further excluding its interaction with adenylate cyclase. Our data suggest that melatonin may play a role in regulating colonic Cl - secretion via melatonin receptors; however, the signal transduction pathway(s) involved remains to be elucidated. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie | en_US |
dc.relation.ispartof | Life Sciences | en_US |
dc.subject | Cl - Secretion | en_US |
dc.subject | Melatonin | en_US |
dc.subject | T 84 Cells | en_US |
dc.title | Effect of melatonin on chloride secretion by human colonic T84 cells | en_US |
dc.type | Article | en_US |
dc.identifier.email | Poon, AMS:amspoon@hkucc.hku.hk | en_US |
dc.identifier.authority | Poon, AMS=rp00354 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/S0024-3205(98)00190-8 | en_US |
dc.identifier.pmid | 9627094 | - |
dc.identifier.scopus | eid_2-s2.0-0031637408 | en_US |
dc.identifier.hkuros | 36963 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0031637408&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 62 | en_US |
dc.identifier.issue | 23 | en_US |
dc.identifier.spage | 2151 | en_US |
dc.identifier.epage | 2158 | en_US |
dc.identifier.isi | WOS:000073527200009 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Chan, HC=7403402737 | en_US |
dc.identifier.scopusauthorid | Lui, KM=7103389986 | en_US |
dc.identifier.scopusauthorid | Wong, WS=7403972688 | en_US |
dc.identifier.scopusauthorid | Poon, AMS=7103068868 | en_US |
dc.customcontrol.immutable | csl 151204 | - |
dc.identifier.issnl | 0024-3205 | - |