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- Publisher Website: 10.1074/jbc.273.23.14566
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- PMID: 9603972
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Article: Calcium signaling by cyclic ADP-ribose, NAADP, and inositol trisphosphate are involved in distinct functions in Ascidian oocytes
Title | Calcium signaling by cyclic ADP-ribose, NAADP, and inositol trisphosphate are involved in distinct functions in Ascidian oocytes |
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Authors | |
Issue Date | 1998 |
Publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ |
Citation | Journal Of Biological Chemistry, 1998, v. 273 n. 23, p. 14566-14574 How to Cite? |
Abstract | ADP-ribosyl cyclase catalyzes the synthesis of two structurally and functionally different Ca2+ releasing molecules, cyclic ADP-ribose (cADPR) from β-NAD and nicotinic acid-adenine dinucleotide phosphate (NAADP) from β-NADP. Their Ca2+-mobilizing effects in ascidian oocytes were characterized in connection with that induced by inositol 1,4,5-trisphosphate (InsP3). Fertilization of the oocyte is accompanied by a decrease in the oocyte Ca2+ current and an increase in membrane capacitance due to the addition of membrane to the cell surface. Both of these electrical changes could be induced by perfusion, through a patch pipette, of nanomolar concentrations of cADPR or its precursor, β-NAD, into unfertilized oocytes. The changes induced by β-NAD showed a distinctive delay consistent with its enzymatic conversion to cADPR. The cADPR-induced changes were inhibited by preloading the oocytes with a Ca2+ chelator, indicating the effects were due to Ca2+ release induced by cADPR. Consistently, ryanodine (at high concentration) or 8-amino-cADPR, a specific antagonist of cADPR, but not heparin, inhibited the cADPR-induced changes. Both inhibitors likewise blocked the membrane insertion that normally occurred at fertilization consistent with it being mediated by a ryanodine receptor. The effects of NAADP were different from those of cADPR. Although NAADP induced a similar decrease in the Ca2+ current, no membrane insertion occurred. Moreover, pretreatment of the oocytes with NAADP inhibited the post-fertilization Ca2+ oscillation while cADPR did not. A similar Ca2+ oscillation could be artificially induced by perfusing into the oocytes a high concentration of InsP3 and NAADP could likewise inhibit such an InsP3-induced oscillation. This work shows that three independent Ca2+ signaling pathways are present in the oocytes and that each is involved in mediating distinct changes associated with fertilization. The results are consistent with a hierarchical organization of Ca2+ stores in the oocyte. |
Persistent Identifier | http://hdl.handle.net/10722/171653 |
ISSN | 2020 Impact Factor: 5.157 2023 SCImago Journal Rankings: 1.766 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Albrieux, M | en_US |
dc.contributor.author | Lee, HC | en_US |
dc.contributor.author | Villaz, M | en_US |
dc.date.accessioned | 2012-10-30T06:16:10Z | - |
dc.date.available | 2012-10-30T06:16:10Z | - |
dc.date.issued | 1998 | en_US |
dc.identifier.citation | Journal Of Biological Chemistry, 1998, v. 273 n. 23, p. 14566-14574 | en_US |
dc.identifier.issn | 0021-9258 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171653 | - |
dc.description.abstract | ADP-ribosyl cyclase catalyzes the synthesis of two structurally and functionally different Ca2+ releasing molecules, cyclic ADP-ribose (cADPR) from β-NAD and nicotinic acid-adenine dinucleotide phosphate (NAADP) from β-NADP. Their Ca2+-mobilizing effects in ascidian oocytes were characterized in connection with that induced by inositol 1,4,5-trisphosphate (InsP3). Fertilization of the oocyte is accompanied by a decrease in the oocyte Ca2+ current and an increase in membrane capacitance due to the addition of membrane to the cell surface. Both of these electrical changes could be induced by perfusion, through a patch pipette, of nanomolar concentrations of cADPR or its precursor, β-NAD, into unfertilized oocytes. The changes induced by β-NAD showed a distinctive delay consistent with its enzymatic conversion to cADPR. The cADPR-induced changes were inhibited by preloading the oocytes with a Ca2+ chelator, indicating the effects were due to Ca2+ release induced by cADPR. Consistently, ryanodine (at high concentration) or 8-amino-cADPR, a specific antagonist of cADPR, but not heparin, inhibited the cADPR-induced changes. Both inhibitors likewise blocked the membrane insertion that normally occurred at fertilization consistent with it being mediated by a ryanodine receptor. The effects of NAADP were different from those of cADPR. Although NAADP induced a similar decrease in the Ca2+ current, no membrane insertion occurred. Moreover, pretreatment of the oocytes with NAADP inhibited the post-fertilization Ca2+ oscillation while cADPR did not. A similar Ca2+ oscillation could be artificially induced by perfusing into the oocytes a high concentration of InsP3 and NAADP could likewise inhibit such an InsP3-induced oscillation. This work shows that three independent Ca2+ signaling pathways are present in the oocytes and that each is involved in mediating distinct changes associated with fertilization. The results are consistent with a hierarchical organization of Ca2+ stores in the oocyte. | en_US |
dc.language | eng | en_US |
dc.publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ | en_US |
dc.relation.ispartof | Journal of Biological Chemistry | en_US |
dc.subject.mesh | Adp-Ribosyl Cyclase | en_US |
dc.subject.mesh | Adenosine Diphosphate Ribose - Analogs & Derivatives - Pharmacology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antigens, Cd | en_US |
dc.subject.mesh | Antigens, Cd38 | en_US |
dc.subject.mesh | Antigens, Differentiation - Metabolism | en_US |
dc.subject.mesh | Calcium - Metabolism | en_US |
dc.subject.mesh | Chelating Agents - Pharmacology | en_US |
dc.subject.mesh | Cyclic Adp-Ribose | en_US |
dc.subject.mesh | Electrophysiology | en_US |
dc.subject.mesh | Fertilization - Physiology | en_US |
dc.subject.mesh | Inositol 1,4,5-Trisphosphate - Pharmacology | en_US |
dc.subject.mesh | Nad - Pharmacology | en_US |
dc.subject.mesh | Nad+ Nucleosidase - Metabolism | en_US |
dc.subject.mesh | Nadp - Analogs & Derivatives - Pharmacology | en_US |
dc.subject.mesh | Oocytes - Metabolism | en_US |
dc.subject.mesh | Patch-Clamp Techniques | en_US |
dc.subject.mesh | Ryanodine - Pharmacology | en_US |
dc.subject.mesh | Signal Transduction - Physiology | en_US |
dc.subject.mesh | Urochordata - Physiology | en_US |
dc.title | Calcium signaling by cyclic ADP-ribose, NAADP, and inositol trisphosphate are involved in distinct functions in Ascidian oocytes | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lee, HC:leehc@hku.hk | en_US |
dc.identifier.authority | Lee, HC=rp00545 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1074/jbc.273.23.14566 | en_US |
dc.identifier.pmid | 9603972 | - |
dc.identifier.scopus | eid_2-s2.0-0032486402 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0032486402&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 273 | en_US |
dc.identifier.issue | 23 | en_US |
dc.identifier.spage | 14566 | en_US |
dc.identifier.epage | 14574 | en_US |
dc.identifier.isi | WOS:000074021500074 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Albrieux, M=6603110547 | en_US |
dc.identifier.scopusauthorid | Lee, HC=26642959100 | en_US |
dc.identifier.scopusauthorid | Villaz, M=7003857998 | en_US |
dc.identifier.issnl | 0021-9258 | - |