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- Publisher Website: 10.1128/JCM.29.11.2424-2433.1991
- Scopus: eid_2-s2.0-0026000613
- PMID: 1723070
- WOS: WOS:A1991GK77700012
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Article: Anti-Salmonella lipopolysaccharide monoclonal antibodies: Characterization of Salmonella BO-, CO-, DO-, and EO-specific clones and their diagnostic usefulness
Title | Anti-Salmonella lipopolysaccharide monoclonal antibodies: Characterization of Salmonella BO-, CO-, DO-, and EO-specific clones and their diagnostic usefulness |
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Authors | |
Issue Date | 1991 |
Citation | Journal Of Clinical Microbiology, 1991, v. 29 n. 11, p. 2424-2433 How to Cite? |
Abstract | To facilitate the identification and serotyping of Salmonella species, we established a wide variety of murine monoclonal antibodies (MAbs) that were reactive with the lipopolysaccharides (LPSs) of Salmonella serogroups B to E. An effective approach for generating LPS-reactive hybridomas was used; this required immunization of mice with LPS-coated bacteria. To screen for diagnostically useful MAbs, the MAbs were tested by enzyme-linked immunosorbent assay against a set of purified LPSs from smooth and rough Salmonella strains. At least four major groups of antibody specificities were identified: Salmonella (i) BO specific, (ii) CO specific, (iii) DO specific, and (iv) EO specific. For a more detailed epitope analysis, a panel of eight different serogroup-specific MAbs which were shown to bind the O-antigenic polysaccharide chains, yielding characteristic ladder patterns in Western blots (immunoblots) against the LPS of Salmonella serogroups B to E, were selected. The availability of various chemically defined LPS structures and Salmonella O-antigen glycoconjugates permitted the definition of O-antigenic polysaccharide epitopes recognized by each MAb that serologically corresponded to factors O3, O4, O5, O6, O7, O8, O9, and O10 on the basis of the Kauffmann-White scheme for Salmonella classification. The diagnostic accuracy of these immunochemically defined O-specific MAbs for Salmonella serotyping was demonstrated by correct identification of all 167 salmonellae (including 72 serotypes from serogroups B to E) among the 294 bacterial strains in a slide agglutination test. No false-positive reactions were detected. |
Persistent Identifier | http://hdl.handle.net/10722/172652 |
ISSN | 2023 Impact Factor: 6.1 2023 SCImago Journal Rankings: 1.653 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Luk, JMC | en_US |
dc.contributor.author | Lindberg, AA | en_US |
dc.date.accessioned | 2012-10-30T06:24:02Z | - |
dc.date.available | 2012-10-30T06:24:02Z | - |
dc.date.issued | 1991 | en_US |
dc.identifier.citation | Journal Of Clinical Microbiology, 1991, v. 29 n. 11, p. 2424-2433 | en_US |
dc.identifier.issn | 0095-1137 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/172652 | - |
dc.description.abstract | To facilitate the identification and serotyping of Salmonella species, we established a wide variety of murine monoclonal antibodies (MAbs) that were reactive with the lipopolysaccharides (LPSs) of Salmonella serogroups B to E. An effective approach for generating LPS-reactive hybridomas was used; this required immunization of mice with LPS-coated bacteria. To screen for diagnostically useful MAbs, the MAbs were tested by enzyme-linked immunosorbent assay against a set of purified LPSs from smooth and rough Salmonella strains. At least four major groups of antibody specificities were identified: Salmonella (i) BO specific, (ii) CO specific, (iii) DO specific, and (iv) EO specific. For a more detailed epitope analysis, a panel of eight different serogroup-specific MAbs which were shown to bind the O-antigenic polysaccharide chains, yielding characteristic ladder patterns in Western blots (immunoblots) against the LPS of Salmonella serogroups B to E, were selected. The availability of various chemically defined LPS structures and Salmonella O-antigen glycoconjugates permitted the definition of O-antigenic polysaccharide epitopes recognized by each MAb that serologically corresponded to factors O3, O4, O5, O6, O7, O8, O9, and O10 on the basis of the Kauffmann-White scheme for Salmonella classification. The diagnostic accuracy of these immunochemically defined O-specific MAbs for Salmonella serotyping was demonstrated by correct identification of all 167 salmonellae (including 72 serotypes from serogroups B to E) among the 294 bacterial strains in a slide agglutination test. No false-positive reactions were detected. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Journal of Clinical Microbiology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antibodies, Bacterial | en_US |
dc.subject.mesh | Antibodies, Monoclonal - Diagnostic Use | en_US |
dc.subject.mesh | Antigens, Bacterial - Chemistry | en_US |
dc.subject.mesh | Carbohydrate Sequence | en_US |
dc.subject.mesh | Epitopes - Chemistry | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Lipopolysaccharides - Chemistry - Immunology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Mice, Inbred Balb C | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Salmonella - Classification - Immunology | en_US |
dc.subject.mesh | Salmonella Infections - Diagnosis | en_US |
dc.subject.mesh | Serotyping | en_US |
dc.title | Anti-Salmonella lipopolysaccharide monoclonal antibodies: Characterization of Salmonella BO-, CO-, DO-, and EO-specific clones and their diagnostic usefulness | en_US |
dc.type | Article | en_US |
dc.identifier.email | Luk, JMC: jmluk@hkucc.hku.hk | en_US |
dc.identifier.authority | Luk, JMC=rp00349 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1128/JCM.29.11.2424-2433.1991 | - |
dc.identifier.pmid | 1723070 | - |
dc.identifier.scopus | eid_2-s2.0-0026000613 | en_US |
dc.identifier.volume | 29 | en_US |
dc.identifier.issue | 11 | en_US |
dc.identifier.spage | 2424 | en_US |
dc.identifier.epage | 2433 | en_US |
dc.identifier.isi | WOS:A1991GK77700012 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Luk, JMC=7006777791 | en_US |
dc.identifier.scopusauthorid | Lindberg, AA=7101776377 | en_US |
dc.identifier.issnl | 0095-1137 | - |