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- Publisher Website: 10.1128/JCM.32.12.3072-3074.1994
- Scopus: eid_2-s2.0-0027959935
- PMID: 7883906
- WOS: WOS:A1994PT18700038
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Article: Comparison of three stool-processing methods for detection of Salmonella serogroups B, C2, and D by PCR
Title | Comparison of three stool-processing methods for detection of Salmonella serogroups B, C2, and D by PCR |
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Authors | |
Issue Date | 1994 |
Citation | Journal Of Clinical Microbiology, 1994, v. 32 n. 12, p. 3072-3074 How to Cite? |
Abstract | Three different stool sample-processing methods (centrifugation, immunomagnetic separation, and selective enrichment cultivation) for the identification of Salmonella serogroups by PCR were studied. The corresponding sensitivities in an ethidium bromide stained-agarose gel were 105, 103, and 10 bacteria, respectively. The PCR assay with overnight enrichment performed as well as, or even better than, the conventional culture technique. Of 485 clinical stool samples, PCR correctly identified all 230 culture-positive samples as well as mixed Salmonella infections in four cases. |
Persistent Identifier | http://hdl.handle.net/10722/172701 |
ISSN | 2023 Impact Factor: 6.1 2023 SCImago Journal Rankings: 1.653 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Kongmuang, U | en_US |
dc.contributor.author | Luk, JMC | en_US |
dc.contributor.author | Lindberg, AA | en_US |
dc.date.accessioned | 2012-10-30T06:24:22Z | - |
dc.date.available | 2012-10-30T06:24:22Z | - |
dc.date.issued | 1994 | en_US |
dc.identifier.citation | Journal Of Clinical Microbiology, 1994, v. 32 n. 12, p. 3072-3074 | en_US |
dc.identifier.issn | 0095-1137 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/172701 | - |
dc.description.abstract | Three different stool sample-processing methods (centrifugation, immunomagnetic separation, and selective enrichment cultivation) for the identification of Salmonella serogroups by PCR were studied. The corresponding sensitivities in an ethidium bromide stained-agarose gel were 105, 103, and 10 bacteria, respectively. The PCR assay with overnight enrichment performed as well as, or even better than, the conventional culture technique. Of 485 clinical stool samples, PCR correctly identified all 230 culture-positive samples as well as mixed Salmonella infections in four cases. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Journal of Clinical Microbiology | en_US |
dc.subject.mesh | Bacteriological Techniques | en_US |
dc.subject.mesh | Base Sequence | en_US |
dc.subject.mesh | Dna, Bacterial - Analysis | en_US |
dc.subject.mesh | Feces - Microbiology | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Polymerase Chain Reaction | en_US |
dc.subject.mesh | Salmonella - Classification - Genetics - Isolation & Purification | en_US |
dc.subject.mesh | Salmonella Infections - Diagnosis | en_US |
dc.title | Comparison of three stool-processing methods for detection of Salmonella serogroups B, C2, and D by PCR | en_US |
dc.type | Article | en_US |
dc.identifier.email | Luk, JMC: jmluk@hkucc.hku.hk | en_US |
dc.identifier.authority | Luk, JMC=rp00349 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1128/JCM.32.12.3072-3074.1994 | - |
dc.identifier.pmid | 7883906 | - |
dc.identifier.scopus | eid_2-s2.0-0027959935 | en_US |
dc.identifier.volume | 32 | en_US |
dc.identifier.issue | 12 | en_US |
dc.identifier.spage | 3072 | en_US |
dc.identifier.epage | 3074 | en_US |
dc.identifier.isi | WOS:A1994PT18700038 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Kongmuang, U=6602328523 | en_US |
dc.identifier.scopusauthorid | Luk, JMC=7006777791 | en_US |
dc.identifier.scopusauthorid | Lindberg, AA=7101776377 | en_US |
dc.identifier.issnl | 0095-1137 | - |