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Article: Different evaluation of human sperm hypoosmotic swelling test and its relationship with the outcome of in-vitro fertilization of human occytes
Title | Different evaluation of human sperm hypoosmotic swelling test and its relationship with the outcome of in-vitro fertilization of human occytes |
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Authors | |
Keywords | Hypoosmotic swelling IVF Multivariate discriminant analysis Sperm morphology |
Issue Date | 1990 |
Publisher | Oxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/ |
Citation | Human Reproduction, 1990, v. 5 n. 1, p. 84-88 How to Cite? |
Abstract | The hypoosmotic swelling test is a simple laboratory test to measure the functional integrity of the human sperm membrane, Its in-vivo and in-vitro applicability needs to be evaluated before it can be accepted as a useful routine test for the fertilizing potential of human seemen. We studied the standard semen analysis results and differential sperm tail swelling patterns of seminal and swim-up sperm after hypoosmotic treatment in 97 semen samples used for in-vitro fertilization of human oocytes. Semen samples were classified as infertile (0% fertilization rate; n = 27) or fertile ( > 0% fertilization rate; n = 70) before statistical analyses. There was a significant difference (P < 0.005) in percentage normal morphology of seminal sperm between the fertile and infertile semen samples. The percentage normal morpholgy of seminal sperm correlated (r = 0.4250; P < 0.005) with the in-vitro fertilization rate of human oocytes and this parameter was selected by the multivariate stepwise discriminant analysis as the discriminator capable of predicting the in-vitro fertilization rate with 57.7% accuracy. The percentage total swelling of seminal and swim-up sperm after hypoosmotic treatment was not correlated with the in-vitro fertilization rate. The percentage swelling pattern g (the open type) of seminal sperm was also selected by the multivaiate stepwise discriminant analysis as the discriminator to predict the in-vitro fertilization rate. This parameter correlated with the percentage normal morphology of seminal sperm (r = 0.3014; P < 0.05). The addition of this parameter to the percentage normal morphology of seminal sperm, another discriminator, did not significantly improve the predicitve accuracy (61.9%) for the in-vitro fertilization rate. Our results indicate that the addition of differential evaluation of the human sperm hypoosmotic swelling test to standard semen analysis is not helpful in predicting the in-vitro fertilization of human oocytes. |
Persistent Identifier | http://hdl.handle.net/10722/173163 |
ISSN | 2023 Impact Factor: 6.0 2023 SCImago Journal Rankings: 1.852 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chan, SYW | en_US |
dc.contributor.author | Wang, C | en_US |
dc.contributor.author | Chan, STH | en_US |
dc.contributor.author | Ho, PC | en_US |
dc.date.accessioned | 2012-10-30T06:28:16Z | - |
dc.date.available | 2012-10-30T06:28:16Z | - |
dc.date.issued | 1990 | en_US |
dc.identifier.citation | Human Reproduction, 1990, v. 5 n. 1, p. 84-88 | en_US |
dc.identifier.issn | 0268-1161 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/173163 | - |
dc.description.abstract | The hypoosmotic swelling test is a simple laboratory test to measure the functional integrity of the human sperm membrane, Its in-vivo and in-vitro applicability needs to be evaluated before it can be accepted as a useful routine test for the fertilizing potential of human seemen. We studied the standard semen analysis results and differential sperm tail swelling patterns of seminal and swim-up sperm after hypoosmotic treatment in 97 semen samples used for in-vitro fertilization of human oocytes. Semen samples were classified as infertile (0% fertilization rate; n = 27) or fertile ( > 0% fertilization rate; n = 70) before statistical analyses. There was a significant difference (P < 0.005) in percentage normal morphology of seminal sperm between the fertile and infertile semen samples. The percentage normal morpholgy of seminal sperm correlated (r = 0.4250; P < 0.005) with the in-vitro fertilization rate of human oocytes and this parameter was selected by the multivariate stepwise discriminant analysis as the discriminator capable of predicting the in-vitro fertilization rate with 57.7% accuracy. The percentage total swelling of seminal and swim-up sperm after hypoosmotic treatment was not correlated with the in-vitro fertilization rate. The percentage swelling pattern g (the open type) of seminal sperm was also selected by the multivaiate stepwise discriminant analysis as the discriminator to predict the in-vitro fertilization rate. This parameter correlated with the percentage normal morphology of seminal sperm (r = 0.3014; P < 0.05). The addition of this parameter to the percentage normal morphology of seminal sperm, another discriminator, did not significantly improve the predicitve accuracy (61.9%) for the in-vitro fertilization rate. Our results indicate that the addition of differential evaluation of the human sperm hypoosmotic swelling test to standard semen analysis is not helpful in predicting the in-vitro fertilization of human oocytes. | en_US |
dc.language | eng | en_US |
dc.publisher | Oxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/ | en_US |
dc.relation.ispartof | Human Reproduction | en_US |
dc.subject | Hypoosmotic swelling | - |
dc.subject | IVF | - |
dc.subject | Multivariate discriminant analysis | - |
dc.subject | Sperm morphology | - |
dc.title | Different evaluation of human sperm hypoosmotic swelling test and its relationship with the outcome of in-vitro fertilization of human occytes | en_US |
dc.type | Article | en_US |
dc.identifier.email | Ho, PC:pcho@hku.hk | en_US |
dc.identifier.authority | Ho, PC=rp00325 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.scopus | eid_2-s2.0-0025193365 | en_US |
dc.identifier.volume | 5 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 84 | en_US |
dc.identifier.epage | 88 | en_US |
dc.identifier.isi | WOS:A1990CP81900017 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Chan, SYW=7404255960 | en_US |
dc.identifier.scopusauthorid | Wang, C=7501631357 | en_US |
dc.identifier.scopusauthorid | Chan, STH=24368283200 | en_US |
dc.identifier.scopusauthorid | Ho, PC=7402211440 | en_US |
dc.identifier.issnl | 0268-1161 | - |