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- Publisher Website: 10.1006/taap.1999.8821
- Scopus: eid_2-s2.0-0033572640
- PMID: 10620487
- WOS: WOS:000085233400010
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Article: Generation and characterization of hammerhead ribozymes targeting rodent metallothionein-I and -II ribonucleic acid
Title | Generation and characterization of hammerhead ribozymes targeting rodent metallothionein-I and -II ribonucleic acid |
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Authors | |
Issue Date | 1999 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/taap |
Citation | Toxicology And Applied Pharmacology, 1999, v. 161 n. 3, p. 294-301 How to Cite? |
Abstract | Two trans-acting hammerhead ribozymes (Rzs), Rz1-2 and Rz4-9, were designed and shown to be effective in cleaving rat or mouse metallothenein (MT)-I and MT-II mRNA, respectively, at position +147/148, in a sequence- specific manner. The catalytic efficiency for Rz1-2 on rat MT-I was found to be 678 M-1s-1 and that for Rz4-9 on rat MT-II cRNA was found to be 372 M-1s-1. An expression vector, pRz(4-9/1-2), containing both Rzs linked in tandem, was constructed and used to transfect NbE-1 cells, an immortalized ventral prostate epithelial cell line. Two stable-transfected lines, NbE- 1(Rz2) and NbE-1(Rz3), expressing substantial levels of (Rz1-2/Rz4-9) and minimal levels of MT-I and MT-II transcripts were found to exhibit increased sensitivity to cadmium (Cd)-induced cytotoxicity compared to the parent line. This article is the first to report successful degradation of rodent MT mRNA in vitro and in cellulo by hammerhead Rzs. |
Persistent Identifier | http://hdl.handle.net/10722/173235 |
ISSN | 2023 Impact Factor: 3.3 2023 SCImago Journal Rankings: 0.788 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, KF | en_US |
dc.contributor.author | Lau, KM | en_US |
dc.contributor.author | Ho, SM | en_US |
dc.date.accessioned | 2012-10-30T06:28:42Z | - |
dc.date.available | 2012-10-30T06:28:42Z | - |
dc.date.issued | 1999 | en_US |
dc.identifier.citation | Toxicology And Applied Pharmacology, 1999, v. 161 n. 3, p. 294-301 | en_US |
dc.identifier.issn | 0041-008X | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/173235 | - |
dc.description.abstract | Two trans-acting hammerhead ribozymes (Rzs), Rz1-2 and Rz4-9, were designed and shown to be effective in cleaving rat or mouse metallothenein (MT)-I and MT-II mRNA, respectively, at position +147/148, in a sequence- specific manner. The catalytic efficiency for Rz1-2 on rat MT-I was found to be 678 M-1s-1 and that for Rz4-9 on rat MT-II cRNA was found to be 372 M-1s-1. An expression vector, pRz(4-9/1-2), containing both Rzs linked in tandem, was constructed and used to transfect NbE-1 cells, an immortalized ventral prostate epithelial cell line. Two stable-transfected lines, NbE- 1(Rz2) and NbE-1(Rz3), expressing substantial levels of (Rz1-2/Rz4-9) and minimal levels of MT-I and MT-II transcripts were found to exhibit increased sensitivity to cadmium (Cd)-induced cytotoxicity compared to the parent line. This article is the first to report successful degradation of rodent MT mRNA in vitro and in cellulo by hammerhead Rzs. | en_US |
dc.language | eng | en_US |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/taap | en_US |
dc.relation.ispartof | Toxicology and Applied Pharmacology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Base Sequence | en_US |
dc.subject.mesh | Cadmium - Toxicity | en_US |
dc.subject.mesh | Cell Line, Transformed | en_US |
dc.subject.mesh | Cell Survival - Drug Effects | en_US |
dc.subject.mesh | Clone Cells | en_US |
dc.subject.mesh | Dna Primers - Chemistry | en_US |
dc.subject.mesh | Epithelial Cells - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Metallothionein - Biosynthesis - Genetics | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Nucleic Acid Conformation | en_US |
dc.subject.mesh | Prostate - Cytology - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Rna, Catalytic - Chemistry - Metabolism | en_US |
dc.subject.mesh | Rna, Messenger - Metabolism | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Transcription, Genetic | en_US |
dc.subject.mesh | Transfection | en_US |
dc.title | Generation and characterization of hammerhead ribozymes targeting rodent metallothionein-I and -II ribonucleic acid | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lee, KF:ckflee@hku.hk | en_US |
dc.identifier.authority | Lee, KF=rp00458 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1006/taap.1999.8821 | en_US |
dc.identifier.pmid | 10620487 | - |
dc.identifier.scopus | eid_2-s2.0-0033572640 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0033572640&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 161 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 294 | en_US |
dc.identifier.epage | 301 | en_US |
dc.identifier.isi | WOS:000085233400010 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Lee, KF=26643097500 | en_US |
dc.identifier.scopusauthorid | Lau, KM=36722691500 | en_US |
dc.identifier.scopusauthorid | Ho, SM=7403713823 | en_US |
dc.identifier.issnl | 0041-008X | - |