File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Scopus: eid_2-s2.0-0024797381
- PMID: 2484708
- WOS: WOS:A1989EC14200017
- Find via
Supplementary
- Citations:
- Appears in Collections:
Conference Paper: SIN-1 stimulates the production of cyclic GMP but not cyclic AMP in porcine aortic endothelial cells
Title | SIN-1 stimulates the production of cyclic GMP but not cyclic AMP in porcine aortic endothelial cells |
---|---|
Authors | |
Keywords | Atrial natriuretic peptide Cyclic AMP Cyclic GMP Endothelial cells Hemoglobin Methylene blue |
Issue Date | 1989 |
Publisher | Lippincott Williams & Wilkins. The Journal's web site is located at http://www.cardiovascularpharm.com/ |
Citation | Journal Of Cardiovascular Pharmacology, 1989, v. 14 SUPPL. 11, p. S91-S94 How to Cite? |
Abstract | The purpose of the present investigations was to determine whether or not SIN-1, a metabolite of molsidomine that spontaneously releases nitric oxide, stimulates the production of adenosine-3',5'-cyclic monophosphate (cyclic AMP) and of guanosine-3',5'-cyclic monophosphate (cyclic GMP) in endothelial cells. All experiments were performed on first or second passage cultured porcine aortic endothelial cells. SIN-1 induced a time- and concentration-dependent accumulation of cyclic GMP but not of cyclic AMP. The production of cyclic GMP evoked by SIN-1 but not evoked by human α-natriuretic polypeptide was inhibited by treatment of the cells with either methylene blue (an inhibitor of soluble guanylate cyclase) and hemoglobin (a scavenger of nitric oxide). These data suggest that SIN-1 enhances the activity of soluble guanylate cyclase, which in turn induces the accumulation of cyclic GMP in endothelial cells. This response is probably due to the spontaneous release of nitric oxide, which is a potent activator of soluble guanylate cyclase. |
Persistent Identifier | http://hdl.handle.net/10722/173457 |
ISSN | 2023 Impact Factor: 2.6 2023 SCImago Journal Rankings: 0.610 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Schini, VB | en_US |
dc.contributor.author | Vanhoutte, PM | en_US |
dc.date.accessioned | 2012-10-30T06:32:10Z | - |
dc.date.available | 2012-10-30T06:32:10Z | - |
dc.date.issued | 1989 | en_US |
dc.identifier.citation | Journal Of Cardiovascular Pharmacology, 1989, v. 14 SUPPL. 11, p. S91-S94 | en_US |
dc.identifier.issn | 0160-2446 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/173457 | - |
dc.description.abstract | The purpose of the present investigations was to determine whether or not SIN-1, a metabolite of molsidomine that spontaneously releases nitric oxide, stimulates the production of adenosine-3',5'-cyclic monophosphate (cyclic AMP) and of guanosine-3',5'-cyclic monophosphate (cyclic GMP) in endothelial cells. All experiments were performed on first or second passage cultured porcine aortic endothelial cells. SIN-1 induced a time- and concentration-dependent accumulation of cyclic GMP but not of cyclic AMP. The production of cyclic GMP evoked by SIN-1 but not evoked by human α-natriuretic polypeptide was inhibited by treatment of the cells with either methylene blue (an inhibitor of soluble guanylate cyclase) and hemoglobin (a scavenger of nitric oxide). These data suggest that SIN-1 enhances the activity of soluble guanylate cyclase, which in turn induces the accumulation of cyclic GMP in endothelial cells. This response is probably due to the spontaneous release of nitric oxide, which is a potent activator of soluble guanylate cyclase. | en_US |
dc.language | eng | en_US |
dc.publisher | Lippincott Williams & Wilkins. The Journal's web site is located at http://www.cardiovascularpharm.com/ | en_US |
dc.relation.ispartof | Journal of Cardiovascular Pharmacology | en_US |
dc.subject | Atrial natriuretic peptide | - |
dc.subject | Cyclic AMP | - |
dc.subject | Cyclic GMP | - |
dc.subject | Endothelial cells | - |
dc.subject | Hemoglobin | - |
dc.subject | Methylene blue | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Aorta, Thoracic | en_US |
dc.subject.mesh | Cells, Cultured | en_US |
dc.subject.mesh | Cyclic Amp - Biosynthesis | en_US |
dc.subject.mesh | Cyclic Gmp - Biosynthesis | en_US |
dc.subject.mesh | Endothelium, Vascular - Metabolism | en_US |
dc.subject.mesh | Heme - Physiology | en_US |
dc.subject.mesh | Methylene Blue | en_US |
dc.subject.mesh | Molsidomine - Analogs & Derivatives - Pharmacology | en_US |
dc.subject.mesh | Rodentia | en_US |
dc.subject.mesh | Vasodilator Agents - Pharmacology | en_US |
dc.title | SIN-1 stimulates the production of cyclic GMP but not cyclic AMP in porcine aortic endothelial cells | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Vanhoutte, PM:vanhoutt@hku.hk | en_US |
dc.identifier.authority | Vanhoutte, PM=rp00238 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 2484708 | - |
dc.identifier.scopus | eid_2-s2.0-0024797381 | en_US |
dc.identifier.volume | 14 | en_US |
dc.identifier.issue | SUPPL. 11 | en_US |
dc.identifier.spage | S91 | en_US |
dc.identifier.epage | S94 | en_US |
dc.identifier.isi | WOS:A1989EC14200017 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Schini, VB=7004113565 | en_US |
dc.identifier.scopusauthorid | Vanhoutte, PM=7202304247 | en_US |
dc.identifier.issnl | 0160-2446 | - |