File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Scopus: eid_2-s2.0-0026322078
- PMID: 1951697
- WOS: WOS:A1991GQ78000050
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Use of the ileal conduit as a model for studying human small intestinal mucus glycoprotein secretion
Title | Use of the ileal conduit as a model for studying human small intestinal mucus glycoprotein secretion |
---|---|
Authors | |
Keywords | Small intestine |
Issue Date | 1991 |
Publisher | American Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/ |
Citation | American Journal Of Physiology - Gastrointestinal And Liver Physiology, 1991, v. 261 n. 5 24-5, p. G728-G734 How to Cite? |
Abstract | To establish a human model system for investigating control of mucus secretion by the small intestine, we have studied the output of mucus glycoprotein in the urine of patients with ileal conduits. A highly specific enzyme-linked immunosorbent assay (ELISA) was used to measure mucus glycoprotein with a sensitivity down to 0.3 ng protein of mucus glycoprotein. The assay detects human ileal mucus glycoprotein in the urine of ileal conduit patients; for an individual subject, the amount hardly varied from day to day, although there were substantial differences between individuals in the amounts secreted. Control urine from persons with normal bladders did not contain any mucus glycoprotein that reacted in the ELISA. The model has many advantages over other experimental systems for studying the rate of mucus glycoprotein secretion from the human ileum and can also be used as a reproducible resource of undegraded mucus glycoprotein. |
Persistent Identifier | http://hdl.handle.net/10722/175668 |
ISSN | |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Roberton, AM | en_US |
dc.contributor.author | Rabel, B | en_US |
dc.contributor.author | Harding, CA | en_US |
dc.contributor.author | TasmanJones, C | en_US |
dc.contributor.author | Harris, PJ | en_US |
dc.contributor.author | Lee, SP | en_US |
dc.date.accessioned | 2012-11-26T09:00:22Z | - |
dc.date.available | 2012-11-26T09:00:22Z | - |
dc.date.issued | 1991 | en_US |
dc.identifier.citation | American Journal Of Physiology - Gastrointestinal And Liver Physiology, 1991, v. 261 n. 5 24-5, p. G728-G734 | en_US |
dc.identifier.issn | 0002-9513 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/175668 | - |
dc.description.abstract | To establish a human model system for investigating control of mucus secretion by the small intestine, we have studied the output of mucus glycoprotein in the urine of patients with ileal conduits. A highly specific enzyme-linked immunosorbent assay (ELISA) was used to measure mucus glycoprotein with a sensitivity down to 0.3 ng protein of mucus glycoprotein. The assay detects human ileal mucus glycoprotein in the urine of ileal conduit patients; for an individual subject, the amount hardly varied from day to day, although there were substantial differences between individuals in the amounts secreted. Control urine from persons with normal bladders did not contain any mucus glycoprotein that reacted in the ELISA. The model has many advantages over other experimental systems for studying the rate of mucus glycoprotein secretion from the human ileum and can also be used as a reproducible resource of undegraded mucus glycoprotein. | en_US |
dc.language | eng | en_US |
dc.publisher | American Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/ | en_US |
dc.relation.ispartof | American Journal of Physiology - Gastrointestinal and Liver Physiology | en_US |
dc.subject | Small intestine | - |
dc.subject.mesh | Amino Acids - Analysis | en_US |
dc.subject.mesh | Anastomosis, Surgical | en_US |
dc.subject.mesh | Cadaver | en_US |
dc.subject.mesh | Electrophoresis, Polyacrylamide Gel | en_US |
dc.subject.mesh | Enzyme-Linked Immunosorbent Assay | en_US |
dc.subject.mesh | Glycoproteins - Chemistry - Secretion - Urine | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Ileum - Secretion | en_US |
dc.subject.mesh | Immunodiffusion | en_US |
dc.subject.mesh | Intestine, Small - Secretion | en_US |
dc.subject.mesh | Monosaccharides - Analysis | en_US |
dc.subject.mesh | Sensitivity And Specificity | en_US |
dc.subject.mesh | Urinary Diversion | en_US |
dc.title | Use of the ileal conduit as a model for studying human small intestinal mucus glycoprotein secretion | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lee, SP: sumlee@hku.hk | en_US |
dc.identifier.authority | Lee, SP=rp01351 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 1951697 | - |
dc.identifier.scopus | eid_2-s2.0-0026322078 | en_US |
dc.identifier.volume | 261 | en_US |
dc.identifier.issue | 5 24-5 | en_US |
dc.identifier.spage | G728 | en_US |
dc.identifier.epage | G734 | en_US |
dc.identifier.isi | WOS:A1991GQ78000050 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Roberton, AM=7003310534 | en_US |
dc.identifier.scopusauthorid | Rabel, B=16158653200 | en_US |
dc.identifier.scopusauthorid | Harding, CA=7102457548 | en_US |
dc.identifier.scopusauthorid | TasmanJones, C=7003303326 | en_US |
dc.identifier.scopusauthorid | Harris, PJ=35512009000 | en_US |
dc.identifier.scopusauthorid | Lee, SP=7601417497 | en_US |
dc.identifier.issnl | 0002-9513 | - |