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- Scopus: eid_2-s2.0-0034047882
- PMID: 10912287
- WOS: WOS:000087833700005
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Article: Effect of polysaccharide krestin on glutathione peroxidase gene expression in mouse peritoneal macrophages
Title | Effect of polysaccharide krestin on glutathione peroxidase gene expression in mouse peritoneal macrophages |
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Authors | |
Keywords | Glutathione peroxidase Glutathione transferase Macrophages Polysaccharides, protein-bound |
Issue Date | 2000 |
Publisher | Step Communications Ltd. The Journal's web site is located at http://www.ibms.org/index.cfm?method=publications.british_journal |
Citation | British Journal Of Biomedical Science, 2000, v. 57 n. 2, p. 130-136 How to Cite? |
Abstract | Polysaccharide krestin (PSK) is a protein-bound polysaccharide extracted from the sporophore Coriolus versicolor. Previously, we found that PSK could reduce the oxidative injury that oxidised low-density lipoprotein (Ox-LDL) produced in monocytes/macrophages, and therefore have some prophylactic or therapeutic effect on atherosclerosis. Glutathione peroxidases, including selenium-dependent glutathione peroxidase (SeGPx) and nonselenium-dependent glutathione peroxidase (non-SeGPx, also called glutathione S-transferase [GST]), play an important role in the defence against oxidative injury. In order to find out if the effects of PSK were associated with antioxidant enzymes, we investigated its effect on glutathione peroxidase activity and messenger RNA (mRNA) expression in mouse peritoneal macrophages. Results showed that PSK enhanced SeGPx and non-SeGPx activity, and increased SeGPx and GST-P (π class GST) mRNA in mouse peritoneal macrophages. In addition, the induction by PSK of the two glutathione peroxidases could be blocked by cycloheximide (30 μg/mL), but 5 μg/mL actinomycin D and 50 μg/mL acetovanilone (a superoxide inhibitor) had no effect. We conclude that PSK improved glutathione peroxidase activity through transcriptional induction of mRNA expression. |
Persistent Identifier | http://hdl.handle.net/10722/178707 |
ISSN | 2023 Impact Factor: 2.7 2023 SCImago Journal Rankings: 0.498 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Pang, ZJ | en_US |
dc.contributor.author | Chen, Y | en_US |
dc.contributor.author | Zhou, M | en_US |
dc.contributor.author | Wan, J | en_US |
dc.date.accessioned | 2012-12-19T09:49:15Z | - |
dc.date.available | 2012-12-19T09:49:15Z | - |
dc.date.issued | 2000 | en_US |
dc.identifier.citation | British Journal Of Biomedical Science, 2000, v. 57 n. 2, p. 130-136 | en_US |
dc.identifier.issn | 0967-4845 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178707 | - |
dc.description.abstract | Polysaccharide krestin (PSK) is a protein-bound polysaccharide extracted from the sporophore Coriolus versicolor. Previously, we found that PSK could reduce the oxidative injury that oxidised low-density lipoprotein (Ox-LDL) produced in monocytes/macrophages, and therefore have some prophylactic or therapeutic effect on atherosclerosis. Glutathione peroxidases, including selenium-dependent glutathione peroxidase (SeGPx) and nonselenium-dependent glutathione peroxidase (non-SeGPx, also called glutathione S-transferase [GST]), play an important role in the defence against oxidative injury. In order to find out if the effects of PSK were associated with antioxidant enzymes, we investigated its effect on glutathione peroxidase activity and messenger RNA (mRNA) expression in mouse peritoneal macrophages. Results showed that PSK enhanced SeGPx and non-SeGPx activity, and increased SeGPx and GST-P (π class GST) mRNA in mouse peritoneal macrophages. In addition, the induction by PSK of the two glutathione peroxidases could be blocked by cycloheximide (30 μg/mL), but 5 μg/mL actinomycin D and 50 μg/mL acetovanilone (a superoxide inhibitor) had no effect. We conclude that PSK improved glutathione peroxidase activity through transcriptional induction of mRNA expression. | en_US |
dc.language | eng | en_US |
dc.publisher | Step Communications Ltd. The Journal's web site is located at http://www.ibms.org/index.cfm?method=publications.british_journal | en_US |
dc.relation.ispartof | British Journal of Biomedical Science | en_US |
dc.subject | Glutathione peroxidase | - |
dc.subject | Glutathione transferase | - |
dc.subject | Macrophages | - |
dc.subject | Polysaccharides, protein-bound | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antibiotics, Antineoplastic - Pharmacology | en_US |
dc.subject.mesh | Gene Expression Regulation, Enzymologic - Drug Effects | en_US |
dc.subject.mesh | Glutathione Peroxidase - Drug Effects - Genetics - Metabolism | en_US |
dc.subject.mesh | Immunologic Factors - Pharmacology | en_US |
dc.subject.mesh | Macrophages, Peritoneal - Drug Effects - Enzymology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Proteoglycans - Pharmacology | en_US |
dc.subject.mesh | Rna, Messenger - Genetics | en_US |
dc.title | Effect of polysaccharide krestin on glutathione peroxidase gene expression in mouse peritoneal macrophages | en_US |
dc.type | Article | en_US |
dc.identifier.email | Wan, J: jmfwan@hku.hk | en_US |
dc.identifier.authority | Wan, J=rp00798 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 10912287 | - |
dc.identifier.scopus | eid_2-s2.0-0034047882 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0034047882&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 57 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 130 | en_US |
dc.identifier.epage | 136 | en_US |
dc.identifier.isi | WOS:000087833700005 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Pang, ZJ=7103343225 | en_US |
dc.identifier.scopusauthorid | Chen, Y=16745998900 | en_US |
dc.identifier.scopusauthorid | Zhou, M=7403506134 | en_US |
dc.identifier.scopusauthorid | Wan, J=8930305000 | en_US |
dc.identifier.issnl | 0967-4845 | - |