File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1007/s002530100786
- Scopus: eid_2-s2.0-0034793636
- PMID: 11759678
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: A perfusion-cell bleeding culture strategy for enhancing the productivity of eicosapentaenoic acid by Nitzschia laevis
Title | A perfusion-cell bleeding culture strategy for enhancing the productivity of eicosapentaenoic acid by Nitzschia laevis |
---|---|
Authors | |
Issue Date | 2001 |
Publisher | Springer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm |
Citation | Applied Microbiology And Biotechnology, 2001, v. 57 n. 3, p. 316-322 How to Cite? |
Abstract | A perfusion-cell bleeding culture strategy was developed for enhancing the productivity of eicosapentaenoic acid (EPA) by the diatom Nitzschia laevis. As the strategy combined the concepts of continuous culture and perfusion culture, it allowed continuously and simultaneously harvesting the algal cells and removing inhibitory compounds during the cultivation. Compared with a single operation of continuous culture, the perfusion-cell bleeding culture greatly enhanced the steady-state biomass concentration, biomass productivity, EPA yield, EPA productivity and glucose utilization efficiency. The perfusion-cell bleeding culture also allowed higher biomass productivity and EPA productivity than the single perfusion culture did. At a bleeding rate of 0.67 day-1 and a perfusion rate of 0.6 day-1, the EPA productivity achieved 175 mg 1-1 day-1, which is the highest ever reported in microalgal cultures. |
Persistent Identifier | http://hdl.handle.net/10722/178725 |
ISSN | 2023 Impact Factor: 3.9 2023 SCImago Journal Rankings: 0.957 |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wen, ZY | en_US |
dc.contributor.author | Chen, F | en_US |
dc.date.accessioned | 2012-12-19T09:49:21Z | - |
dc.date.available | 2012-12-19T09:49:21Z | - |
dc.date.issued | 2001 | en_US |
dc.identifier.citation | Applied Microbiology And Biotechnology, 2001, v. 57 n. 3, p. 316-322 | en_US |
dc.identifier.issn | 0175-7598 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178725 | - |
dc.description.abstract | A perfusion-cell bleeding culture strategy was developed for enhancing the productivity of eicosapentaenoic acid (EPA) by the diatom Nitzschia laevis. As the strategy combined the concepts of continuous culture and perfusion culture, it allowed continuously and simultaneously harvesting the algal cells and removing inhibitory compounds during the cultivation. Compared with a single operation of continuous culture, the perfusion-cell bleeding culture greatly enhanced the steady-state biomass concentration, biomass productivity, EPA yield, EPA productivity and glucose utilization efficiency. The perfusion-cell bleeding culture also allowed higher biomass productivity and EPA productivity than the single perfusion culture did. At a bleeding rate of 0.67 day-1 and a perfusion rate of 0.6 day-1, the EPA productivity achieved 175 mg 1-1 day-1, which is the highest ever reported in microalgal cultures. | en_US |
dc.language | eng | en_US |
dc.publisher | Springer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm | en_US |
dc.relation.ispartof | Applied Microbiology and Biotechnology | en_US |
dc.subject.mesh | Biomass | en_US |
dc.subject.mesh | Bioreactors | en_US |
dc.subject.mesh | Diatoms - Growth & Development - Metabolism | en_US |
dc.subject.mesh | Eicosapentaenoic Acid - Analysis - Biosynthesis | en_US |
dc.title | A perfusion-cell bleeding culture strategy for enhancing the productivity of eicosapentaenoic acid by Nitzschia laevis | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_US |
dc.identifier.authority | Chen, F=rp00672 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1007/s002530100786 | en_US |
dc.identifier.pmid | 11759678 | - |
dc.identifier.scopus | eid_2-s2.0-0034793636 | en_US |
dc.identifier.hkuros | 72409 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0034793636&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 57 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 316 | en_US |
dc.identifier.epage | 322 | en_US |
dc.publisher.place | Germany | en_US |
dc.identifier.scopusauthorid | Wen, ZY=14012373200 | en_US |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_US |
dc.identifier.issnl | 0175-7598 | - |