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Article: Effects of pituitary adenylate cyclase activating polypeptide (PACAP) on intracellular cAMP and Ca 2+ levels in common carp (Cyprinus carpio) pituitary cells in vitro
Title | Effects of pituitary adenylate cyclase activating polypeptide (PACAP) on intracellular cAMP and Ca 2+ levels in common carp (Cyprinus carpio) pituitary cells in vitro |
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Authors | |
Keywords | Calcium cAMP Common carp Pituitary adenylate cyclase activating polypeptide (PACAP) Signal transduction |
Issue Date | 2002 |
Citation | Acta Biochimica Et Biophysica Sinica, 2002, v. 34 n. 6, p. 790-795 How to Cite? |
Abstract | In order to study signal transduction in PACAP-stimulated GH release, effects of ovine PACAPs (oPACAPs) on cAMP synthesis and intracellular free Ca 2+ ([Ca 2+ ] i) levels in primarily cultured common carp (Cyprinus carpio) pituitary cells were investigated using radioimmunoassay (RIA) for cAMP and [Ca 2+ ] i Indo1/AM loading techniques. The main results obtained could be briefly summarized as follows: parallel with their GH-releasing effects, oPACAP-38 (0.01 nmol/L to 1 μmol/L) and oPACAP-27 (0.01 nmol/L to 1 μmol/L) were also effective in increasing cAMP release, cellular cAMP content, and total cAMP production in a dose-dependent manner in common carp pituitary cells. In addition to their cAMP synthesis, 0.5 μmol/L oPACAP-38 and 0.5 μmol/L oPACAP-27 were also effective in elevating [Ca 2+ ] i levels in common carp pituitary cells preloaded with the Ca 2+ -sensitive dye Indo-1; the stimulatory actions were dependent on the presence of extracellular Ca 2+ ([Ca 2+ ] e), as removal of [Ca 2+ ] e by adding the Ca 2+ chelator 4 mmol/L EGTA blocked the rise of [Ca 2+ ] i induced by oPACAP-38 and oPACAP-27, respectively. Besides, the rise in [Ca 2+ ] i induced by 0.5 μmol/L oPACAP-38 was blocked by treatment with 10 μmol/L nifedipine, an inhibitor of "L-type" voltage-sensitive Ca 2+ channels (VSCC); but 0.5 μmol/L oPACAP-38 was not effective in elevating [Ca 2+ ] i levels after the cells treated with Ca 2+ -sensitive dye Indo-1 were challenged with 10 μmol/L nifedipine. These results, taken as a whole, strongly suggest the involvement of the cAMP- and Ca 2+ -dependent pathways in PACAP-stimulated GH release in common carp. |
Persistent Identifier | http://hdl.handle.net/10722/178778 |
ISSN | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Xiao, D | en_US |
dc.contributor.author | Chu, MS | en_US |
dc.contributor.author | Lee, KY | en_US |
dc.contributor.author | Wong, AO | en_US |
dc.contributor.author | Lin, HR | en_US |
dc.date.accessioned | 2012-12-19T09:49:42Z | - |
dc.date.available | 2012-12-19T09:49:42Z | - |
dc.date.issued | 2002 | en_US |
dc.identifier.citation | Acta Biochimica Et Biophysica Sinica, 2002, v. 34 n. 6, p. 790-795 | en_US |
dc.identifier.issn | 0582-9879 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178778 | - |
dc.description.abstract | In order to study signal transduction in PACAP-stimulated GH release, effects of ovine PACAPs (oPACAPs) on cAMP synthesis and intracellular free Ca 2+ ([Ca 2+ ] i) levels in primarily cultured common carp (Cyprinus carpio) pituitary cells were investigated using radioimmunoassay (RIA) for cAMP and [Ca 2+ ] i Indo1/AM loading techniques. The main results obtained could be briefly summarized as follows: parallel with their GH-releasing effects, oPACAP-38 (0.01 nmol/L to 1 μmol/L) and oPACAP-27 (0.01 nmol/L to 1 μmol/L) were also effective in increasing cAMP release, cellular cAMP content, and total cAMP production in a dose-dependent manner in common carp pituitary cells. In addition to their cAMP synthesis, 0.5 μmol/L oPACAP-38 and 0.5 μmol/L oPACAP-27 were also effective in elevating [Ca 2+ ] i levels in common carp pituitary cells preloaded with the Ca 2+ -sensitive dye Indo-1; the stimulatory actions were dependent on the presence of extracellular Ca 2+ ([Ca 2+ ] e), as removal of [Ca 2+ ] e by adding the Ca 2+ chelator 4 mmol/L EGTA blocked the rise of [Ca 2+ ] i induced by oPACAP-38 and oPACAP-27, respectively. Besides, the rise in [Ca 2+ ] i induced by 0.5 μmol/L oPACAP-38 was blocked by treatment with 10 μmol/L nifedipine, an inhibitor of "L-type" voltage-sensitive Ca 2+ channels (VSCC); but 0.5 μmol/L oPACAP-38 was not effective in elevating [Ca 2+ ] i levels after the cells treated with Ca 2+ -sensitive dye Indo-1 were challenged with 10 μmol/L nifedipine. These results, taken as a whole, strongly suggest the involvement of the cAMP- and Ca 2+ -dependent pathways in PACAP-stimulated GH release in common carp. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Acta Biochimica et Biophysica Sinica | en_US |
dc.subject | Calcium | - |
dc.subject | cAMP | - |
dc.subject | Common carp | - |
dc.subject | Pituitary adenylate cyclase activating polypeptide (PACAP) | - |
dc.subject | Signal transduction | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Calcium - Metabolism | en_US |
dc.subject.mesh | Calcium Channel Blockers - Pharmacology | en_US |
dc.subject.mesh | Carps | en_US |
dc.subject.mesh | Cells, Cultured | en_US |
dc.subject.mesh | Cyclic Amp - Metabolism | en_US |
dc.subject.mesh | Dose-Response Relationship, Drug | en_US |
dc.subject.mesh | Neuropeptides - Pharmacology | en_US |
dc.subject.mesh | Nifedipine - Pharmacology | en_US |
dc.subject.mesh | Pituitary Adenylate Cyclase-Activating Polypeptide | en_US |
dc.subject.mesh | Pituitary Gland - Cytology - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Sheep | en_US |
dc.subject.mesh | Time Factors | en_US |
dc.title | Effects of pituitary adenylate cyclase activating polypeptide (PACAP) on intracellular cAMP and Ca 2+ levels in common carp (Cyprinus carpio) pituitary cells in vitro | en_US |
dc.type | Article | en_US |
dc.identifier.email | Wong, AO: olwong@hkucc.hku.hk | en_US |
dc.identifier.authority | Wong, AO=rp00806 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 12417926 | - |
dc.identifier.scopus | eid_2-s2.0-0036944502 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0036944502&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 34 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.spage | 790 | en_US |
dc.identifier.epage | 795 | en_US |
dc.identifier.scopusauthorid | Xiao, D=45561966600 | en_US |
dc.identifier.scopusauthorid | Chu, MS=7402457255 | en_US |
dc.identifier.scopusauthorid | Lee, KY=26660356800 | en_US |
dc.identifier.scopusauthorid | Wong, AO=7403147570 | en_US |
dc.identifier.scopusauthorid | Lin, HR=7405571313 | en_US |
dc.identifier.issnl | 0582-9879 | - |