File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/S0032-9592(02)00174-7
- Scopus: eid_2-s2.0-0037010767
- WOS: WOS:000179438000007
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Perfusion culture of the diatom Nitzschia laevis for ultra-high yield of eicosapentaenoic acid
Title | Perfusion culture of the diatom Nitzschia laevis for ultra-high yield of eicosapentaenoic acid |
---|---|
Authors | |
Keywords | Diatom Eicosapentaenoic Acid High Yield Nitzschia Laevis Perfusion |
Issue Date | 2002 |
Publisher | Elsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/procbio |
Citation | Process Biochemistry, 2002, v. 38 n. 4, p. 523-529 How to Cite? |
Abstract | In the development of high yield of eicosapentaenoic acid (EPA) by the diatom Nitzschia laevis, substrate limitation and toxic metabolite inhibition were the two principal obstacles. In the present study, a perfusion culture was developed to eliminate substrate (glucose) limitation and by-product inhibition simultaneously. Using an exponential feed strategy and manipulating glucose supply rate and by-product removal rate, the optimal glucose concentration in the feed medium was determined to be 50 g l-1. With this feed glucose concentration (S0 = 50 g l-1), the perfusion culture resulted to a cell dry weight concentration of 40 g l-1. Such a high cell density led to an EPA yield of 1112 mg l-1, which is the highest ever reported in microalgal cultures. In addition, the perfusion culture (at S0 = 50 g l-1) greatly reduced the amount of wastewater (broth) during cultures. Considering the simple set-up and operation, the proposed perfusion culture is potentially useful for large scale EPA production by N. laevis. © 2002 Elsevier Science Ltd. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/178779 |
ISSN | 2023 Impact Factor: 3.7 2023 SCImago Journal Rankings: 0.701 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wen, ZY | en_US |
dc.contributor.author | Chen, F | en_US |
dc.date.accessioned | 2012-12-19T09:49:42Z | - |
dc.date.available | 2012-12-19T09:49:42Z | - |
dc.date.issued | 2002 | en_US |
dc.identifier.citation | Process Biochemistry, 2002, v. 38 n. 4, p. 523-529 | en_US |
dc.identifier.issn | 1359-5113 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178779 | - |
dc.description.abstract | In the development of high yield of eicosapentaenoic acid (EPA) by the diatom Nitzschia laevis, substrate limitation and toxic metabolite inhibition were the two principal obstacles. In the present study, a perfusion culture was developed to eliminate substrate (glucose) limitation and by-product inhibition simultaneously. Using an exponential feed strategy and manipulating glucose supply rate and by-product removal rate, the optimal glucose concentration in the feed medium was determined to be 50 g l-1. With this feed glucose concentration (S0 = 50 g l-1), the perfusion culture resulted to a cell dry weight concentration of 40 g l-1. Such a high cell density led to an EPA yield of 1112 mg l-1, which is the highest ever reported in microalgal cultures. In addition, the perfusion culture (at S0 = 50 g l-1) greatly reduced the amount of wastewater (broth) during cultures. Considering the simple set-up and operation, the proposed perfusion culture is potentially useful for large scale EPA production by N. laevis. © 2002 Elsevier Science Ltd. All rights reserved. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/procbio | en_US |
dc.relation.ispartof | Process Biochemistry | en_US |
dc.rights | Process Biochemistry. Copyright © Elsevier Ltd. | - |
dc.subject | Diatom | en_US |
dc.subject | Eicosapentaenoic Acid | en_US |
dc.subject | High Yield | en_US |
dc.subject | Nitzschia Laevis | en_US |
dc.subject | Perfusion | en_US |
dc.title | Perfusion culture of the diatom Nitzschia laevis for ultra-high yield of eicosapentaenoic acid | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_US |
dc.identifier.authority | Chen, F=rp00672 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/S0032-9592(02)00174-7 | en_US |
dc.identifier.scopus | eid_2-s2.0-0037010767 | en_US |
dc.identifier.hkuros | 80809 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0037010767&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 38 | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.spage | 523 | en_US |
dc.identifier.epage | 529 | en_US |
dc.identifier.isi | WOS:000179438000007 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Wen, ZY=14012373200 | en_US |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_US |
dc.identifier.issnl | 1359-5113 | - |