File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/0960-8524(95)00141-7
- Scopus: eid_2-s2.0-0343773296
- WOS: WOS:A1996UP45300002
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: High cell density culture of Chlamydomonas reinhardtii on acetate using fed-batch and hollow-fibre cell-recycle systems
Title | High cell density culture of Chlamydomonas reinhardtii on acetate using fed-batch and hollow-fibre cell-recycle systems |
---|---|
Authors | |
Keywords | Acetate Chlamydomonas Reinhardtii Fed Batch High Cell Density Hollow-Fibre Cell-Recycle System |
Issue Date | 1996 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biortech |
Citation | Bioresource Technology, 1996, v. 55 n. 2, p. 103-110 How to Cite? |
Abstract | Fed-batch culture was performed to minimise growth inhibition of Chlamydomonas reinhardtii by acetate in heterotrophic culture. Although the cell concentration obtained was higher than in batch cultures it was eventually constrained by high sodium concentrations, which accumulated in the fermenter. The use of a continuous-flow, hollow-fibre cell-recycle system (HFCRS) using a membrane with a 0.2 μm pore size was successfully employed to overcome this problem. The HFCRS was run with no cell bleed to obtain a cell concentration of 9 g/l at a dilution rate of 0.1/h and a feed acetate concentration of 1.7 g/l. However, the cell growth yield under these conditions was poor. Using the same dilution rate and feed acetate concentration, a superior cell productivity of 0.083 g/l/h was achieved at a bleed ratio of 0.5. An unstructured mathematical model, incorporating the maintenance coefficient, was derived for the HFCRS. The model gave good agreement with experimental results, particularly at high bleed ratios, and predicted that higher cell concentration and productivity could be obtained using the HFCRS with a higher feed acetate concentration and dilution rate. The results demonstrate that the HFCRS is well suited to obtaining high concentrations of microalgae grown heterotrophically on inhibitory substrates. | Fed-batch culture was performed to minimise growth inhibition of Chlamydomonas reinhardtii by acetate in heterotrophic culture. Although the cell concentration obtained was higher than in batch cultures, it was eventually constrained by high sodium concentrations, which accumulated in the fermenter. The use of a continuous-flow, hollow-fibre cell-recycle system (HFCRS) using a membrane with a 0·2 μm pore size was successfully employed to overcome this problem. The HFCRS was run with no cell bleed to obtain a cell concentration of 9 g/l at a dilution rate of 0·1/h and a feed acetate concentration of 1·7 g/l. However, the cell growth yield under these conditions was poor. Using the same dilution rate and feed acetate concentration, a superior cell productivity of 0·083 g/l/h was achieved at a bleed ratio of 0·5. An unstructured mathematical model, incorporating the maintenance coefficient, was derived for the HFCRS. The model gave good agreement with experimental results, particularly at high bleed ratios, and predicted that higher cell concentration and productivity could be obtained using the HFCRS with a higher feed acetate concentration and dilution rate. The results demonstrate that the HFCRS is well suited to obtaining high concentrations of microalgae grown heterotrophically on inhibitory substrates. |
Persistent Identifier | http://hdl.handle.net/10722/178826 |
ISSN | 2023 Impact Factor: 9.7 2023 SCImago Journal Rankings: 2.576 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chen, F | en_US |
dc.contributor.author | Johns, MR | en_US |
dc.date.accessioned | 2012-12-19T09:49:58Z | - |
dc.date.available | 2012-12-19T09:49:58Z | - |
dc.date.issued | 1996 | en_US |
dc.identifier.citation | Bioresource Technology, 1996, v. 55 n. 2, p. 103-110 | en_US |
dc.identifier.issn | 0960-8524 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178826 | - |
dc.description.abstract | Fed-batch culture was performed to minimise growth inhibition of Chlamydomonas reinhardtii by acetate in heterotrophic culture. Although the cell concentration obtained was higher than in batch cultures it was eventually constrained by high sodium concentrations, which accumulated in the fermenter. The use of a continuous-flow, hollow-fibre cell-recycle system (HFCRS) using a membrane with a 0.2 μm pore size was successfully employed to overcome this problem. The HFCRS was run with no cell bleed to obtain a cell concentration of 9 g/l at a dilution rate of 0.1/h and a feed acetate concentration of 1.7 g/l. However, the cell growth yield under these conditions was poor. Using the same dilution rate and feed acetate concentration, a superior cell productivity of 0.083 g/l/h was achieved at a bleed ratio of 0.5. An unstructured mathematical model, incorporating the maintenance coefficient, was derived for the HFCRS. The model gave good agreement with experimental results, particularly at high bleed ratios, and predicted that higher cell concentration and productivity could be obtained using the HFCRS with a higher feed acetate concentration and dilution rate. The results demonstrate that the HFCRS is well suited to obtaining high concentrations of microalgae grown heterotrophically on inhibitory substrates. | Fed-batch culture was performed to minimise growth inhibition of Chlamydomonas reinhardtii by acetate in heterotrophic culture. Although the cell concentration obtained was higher than in batch cultures, it was eventually constrained by high sodium concentrations, which accumulated in the fermenter. The use of a continuous-flow, hollow-fibre cell-recycle system (HFCRS) using a membrane with a 0·2 μm pore size was successfully employed to overcome this problem. The HFCRS was run with no cell bleed to obtain a cell concentration of 9 g/l at a dilution rate of 0·1/h and a feed acetate concentration of 1·7 g/l. However, the cell growth yield under these conditions was poor. Using the same dilution rate and feed acetate concentration, a superior cell productivity of 0·083 g/l/h was achieved at a bleed ratio of 0·5. An unstructured mathematical model, incorporating the maintenance coefficient, was derived for the HFCRS. The model gave good agreement with experimental results, particularly at high bleed ratios, and predicted that higher cell concentration and productivity could be obtained using the HFCRS with a higher feed acetate concentration and dilution rate. The results demonstrate that the HFCRS is well suited to obtaining high concentrations of microalgae grown heterotrophically on inhibitory substrates. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biortech | en_US |
dc.relation.ispartof | Bioresource Technology | en_US |
dc.subject | Acetate | en_US |
dc.subject | Chlamydomonas Reinhardtii | en_US |
dc.subject | Fed Batch | en_US |
dc.subject | High Cell Density | en_US |
dc.subject | Hollow-Fibre Cell-Recycle System | en_US |
dc.title | High cell density culture of Chlamydomonas reinhardtii on acetate using fed-batch and hollow-fibre cell-recycle systems | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_US |
dc.identifier.authority | Chen, F=rp00672 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/0960-8524(95)00141-7 | en_US |
dc.identifier.scopus | eid_2-s2.0-0343773296 | en_US |
dc.identifier.hkuros | 12305 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0343773296&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 55 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 103 | en_US |
dc.identifier.epage | 110 | en_US |
dc.identifier.isi | WOS:A1996UP45300002 | - |
dc.publisher.place | Netherlands | en_US |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_US |
dc.identifier.scopusauthorid | Johns, MR=7202660598 | en_US |
dc.identifier.issnl | 0960-8524 | - |