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Article: α2-Macroglobulin expression in the liver in response to inflammation is mediated by the testis
Title | α2-Macroglobulin expression in the liver in response to inflammation is mediated by the testis |
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Authors | |
Issue Date | 2005 |
Publisher | Society for Endocrinology. The Journal's web site is located at http://joe.endocrinology-journals.org |
Citation | Journal Of Endocrinology, 2005, v. 185 n. 3, p. 497-505 How to Cite? |
Abstract | Earlier studies have shown that germ cells or germ cell-conditioned media are capable of regulating α2-macroglobulin (α2-MG, a non-specific protease inhibitor) expression by Sertoli cells and hepatocytes cultured in vitro. These results illustrate a possible physiological link between testes and liver regarding α2-MG production. Using a series of surgical procedures including castration, hemicastration, and hepatectomy coupled with Northern blot and immunoblot analyses, we report herein that the surge in α2-MG expression in the liver in response to inflammation is indeed regulated, at least in part, by the testis via testosterone. It was found that hepatectomy induced at least a tenfold increase in the steady-state mRNA and protein production of α2-MG in the liver. However, castration induced a mild but not statistically significant induction of α2-MG in the liver in contrast to sham operation or hemicastration alone, when hemicastration alone could induce liver α2-MG production by almost fourfold. Perhaps most important of all, hepatectomy accompanied by castration significantly reduced the liver α2-MG response to the surgery-induced inflammation compared with hepatectomy alone, illustrating that the removal of the testicles can induce a loss of signal communications between the testis and the liver, rendering a significant loss of the α2-MG response to experimentally induced inflammation in the liver. Interestingly, this lack of response of the liver to surgery-induced inflammation regarding α2-MG production following castration could be restored, at least in part, by using testosterone implants placed subdermally 6 days prior to orchiectomy. Collectively, these results illustrate that a physiological link does indeed exist between the testis and the liver, and that testes per se can influence the liver α2-MG expression in response to inflammation in vivo possibly via testosterone or testosterone-induced biological factor(s). © 2005 Society for Endocrinology. |
Persistent Identifier | http://hdl.handle.net/10722/178891 |
ISSN | 2023 Impact Factor: 3.4 2023 SCImago Journal Rankings: 1.159 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lui, WY | en_US |
dc.contributor.author | Cheng, YH | en_US |
dc.contributor.author | Mruk, DD | en_US |
dc.contributor.author | Cheng, CH | en_US |
dc.contributor.author | Mo, MY | en_US |
dc.contributor.author | Lee, WM | en_US |
dc.contributor.author | Cheng, CY | en_US |
dc.date.accessioned | 2012-12-19T09:50:30Z | - |
dc.date.available | 2012-12-19T09:50:30Z | - |
dc.date.issued | 2005 | en_US |
dc.identifier.citation | Journal Of Endocrinology, 2005, v. 185 n. 3, p. 497-505 | en_US |
dc.identifier.issn | 0022-0795 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178891 | - |
dc.description.abstract | Earlier studies have shown that germ cells or germ cell-conditioned media are capable of regulating α2-macroglobulin (α2-MG, a non-specific protease inhibitor) expression by Sertoli cells and hepatocytes cultured in vitro. These results illustrate a possible physiological link between testes and liver regarding α2-MG production. Using a series of surgical procedures including castration, hemicastration, and hepatectomy coupled with Northern blot and immunoblot analyses, we report herein that the surge in α2-MG expression in the liver in response to inflammation is indeed regulated, at least in part, by the testis via testosterone. It was found that hepatectomy induced at least a tenfold increase in the steady-state mRNA and protein production of α2-MG in the liver. However, castration induced a mild but not statistically significant induction of α2-MG in the liver in contrast to sham operation or hemicastration alone, when hemicastration alone could induce liver α2-MG production by almost fourfold. Perhaps most important of all, hepatectomy accompanied by castration significantly reduced the liver α2-MG response to the surgery-induced inflammation compared with hepatectomy alone, illustrating that the removal of the testicles can induce a loss of signal communications between the testis and the liver, rendering a significant loss of the α2-MG response to experimentally induced inflammation in the liver. Interestingly, this lack of response of the liver to surgery-induced inflammation regarding α2-MG production following castration could be restored, at least in part, by using testosterone implants placed subdermally 6 days prior to orchiectomy. Collectively, these results illustrate that a physiological link does indeed exist between the testis and the liver, and that testes per se can influence the liver α2-MG expression in response to inflammation in vivo possibly via testosterone or testosterone-induced biological factor(s). © 2005 Society for Endocrinology. | en_US |
dc.language | eng | en_US |
dc.publisher | Society for Endocrinology. The Journal's web site is located at http://joe.endocrinology-journals.org | en_US |
dc.relation.ispartof | Journal of Endocrinology | en_US |
dc.rights | Journal of Endocrinology. Copyright © Society for Endocrinology. | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Drug Implants | en_US |
dc.subject.mesh | Gene Expression Regulation | en_US |
dc.subject.mesh | Hepatectomy | en_US |
dc.subject.mesh | Immunoblotting | en_US |
dc.subject.mesh | Inflammation | en_US |
dc.subject.mesh | Liver - Metabolism | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Orchiectomy | en_US |
dc.subject.mesh | Rna, Messenger - Analysis | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Sprague-Dawley | en_US |
dc.subject.mesh | Surgical Wound Infection - Metabolism | en_US |
dc.subject.mesh | Testis - Metabolism | en_US |
dc.subject.mesh | Testosterone - Pharmacology | en_US |
dc.subject.mesh | Alpha-Macroglobulins - Analysis - Genetics - Metabolism | en_US |
dc.title | α2-Macroglobulin expression in the liver in response to inflammation is mediated by the testis | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lui, WY: wylui@hku.hk | en_US |
dc.identifier.email | Lee, WM: hrszlwm@hku.hk | en_US |
dc.identifier.authority | Lui, WY=rp00756 | en_US |
dc.identifier.authority | Lee, WM=rp00728 | en_US |
dc.description.nature | link_to_OA_fulltext | en_US |
dc.identifier.doi | 10.1677/joe.1.06136 | en_US |
dc.identifier.pmid | 15930176 | - |
dc.identifier.scopus | eid_2-s2.0-21244485432 | en_US |
dc.identifier.hkuros | 100001 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-21244485432&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 185 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 497 | en_US |
dc.identifier.epage | 505 | en_US |
dc.identifier.isi | WOS:000229857800016 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Lui, WY=35220192400 | en_US |
dc.identifier.scopusauthorid | Cheng, YH=37093704600 | en_US |
dc.identifier.scopusauthorid | Mruk, DD=6701823934 | en_US |
dc.identifier.scopusauthorid | Cheng, CH=8630373700 | en_US |
dc.identifier.scopusauthorid | Mo, MY=7006857340 | en_US |
dc.identifier.scopusauthorid | Lee, WM=24799156600 | en_US |
dc.identifier.scopusauthorid | Cheng, CY=7404797787 | en_US |
dc.identifier.issnl | 0022-0795 | - |