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- Publisher Website: 10.1016/j.carres.2009.05.032
- Scopus: eid_2-s2.0-68949167939
- PMID: 19604502
- WOS: WOS:000269991600016
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Article: Fine structure characterization of amylopectins from grain amaranth starch
Title | Fine structure characterization of amylopectins from grain amaranth starch |
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Authors | |
Keywords | Amaranthus Amylopectin Building blocks Cluster structure Starch |
Issue Date | 2009 |
Publisher | Pergamon. The Journal's web site is located at http://www.elsevier.com/locate/carres |
Citation | Carbohydrate Research, 2009, v. 344 n. 13, p. 1701-1708 How to Cite? |
Abstract | The aim of this study was to determine the fine structure of amylopectin from grain amaranth. Amaranthus amylopectin was hydrolyzed with α-amylase, and single clusters and a group of clusters (domain) were isolated by methanol precipitation. The domain and the clusters were treated with phosphorylase a and then β-amylase to remove all external chains, whereby the internal structure was obtained. The φ{symbol},β-limit dextrins were analyzed on Sepharose CL 6B. The average DP (degree of polymerization) and peak-DP values of fractions of clusters were 57 and 82, respectively; the values of the domain were 137 and 309, respectively. The unit chain length profiles were analyzed by high-performance anion-exchange chromatography with pulsed amperometric detector (HPAEC-PAD). The results showed that the domain fraction contained ∼2.2 clusters, and single clusters were composed of ∼13 chains. The φ{symbol},β-limit dextrins of the clusters were further hydrolyzed with α-amylase to characterize their building block composition. The average DP of the branched blocks was ∼11 and they contained on average ∼2.5 chains. Their average chain length, internal chain length, and degree of branching were approximately 4.3, 2.8, and 14, respectively. A cluster consisted of ∼6 branched blocks, and the internal chain length between the blocks was ∼6.8. © 2009 Elsevier Ltd. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/179153 |
ISSN | 2023 Impact Factor: 2.4 2023 SCImago Journal Rankings: 0.509 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kong, X | en_US |
dc.contributor.author | Corke, H | en_US |
dc.contributor.author | Bertoft, E | en_US |
dc.date.accessioned | 2012-12-19T09:52:24Z | - |
dc.date.available | 2012-12-19T09:52:24Z | - |
dc.date.issued | 2009 | en_US |
dc.identifier.citation | Carbohydrate Research, 2009, v. 344 n. 13, p. 1701-1708 | en_US |
dc.identifier.issn | 0008-6215 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179153 | - |
dc.description.abstract | The aim of this study was to determine the fine structure of amylopectin from grain amaranth. Amaranthus amylopectin was hydrolyzed with α-amylase, and single clusters and a group of clusters (domain) were isolated by methanol precipitation. The domain and the clusters were treated with phosphorylase a and then β-amylase to remove all external chains, whereby the internal structure was obtained. The φ{symbol},β-limit dextrins were analyzed on Sepharose CL 6B. The average DP (degree of polymerization) and peak-DP values of fractions of clusters were 57 and 82, respectively; the values of the domain were 137 and 309, respectively. The unit chain length profiles were analyzed by high-performance anion-exchange chromatography with pulsed amperometric detector (HPAEC-PAD). The results showed that the domain fraction contained ∼2.2 clusters, and single clusters were composed of ∼13 chains. The φ{symbol},β-limit dextrins of the clusters were further hydrolyzed with α-amylase to characterize their building block composition. The average DP of the branched blocks was ∼11 and they contained on average ∼2.5 chains. Their average chain length, internal chain length, and degree of branching were approximately 4.3, 2.8, and 14, respectively. A cluster consisted of ∼6 branched blocks, and the internal chain length between the blocks was ∼6.8. © 2009 Elsevier Ltd. All rights reserved. | en_US |
dc.language | eng | en_US |
dc.publisher | Pergamon. The Journal's web site is located at http://www.elsevier.com/locate/carres | en_US |
dc.relation.ispartof | Carbohydrate Research | en_US |
dc.subject | Amaranthus | - |
dc.subject | Amylopectin | - |
dc.subject | Building blocks | - |
dc.subject | Cluster structure | - |
dc.subject | Starch | - |
dc.subject.mesh | Amaranthus - Chemistry | en_US |
dc.subject.mesh | Amylopectin - Chemistry - Metabolism | en_US |
dc.subject.mesh | Dextrins - Chemistry | en_US |
dc.subject.mesh | Kinetics | en_US |
dc.subject.mesh | Molecular Weight | en_US |
dc.subject.mesh | Starch - Chemistry | en_US |
dc.subject.mesh | Alpha-Amylases - Metabolism | en_US |
dc.title | Fine structure characterization of amylopectins from grain amaranth starch | en_US |
dc.type | Article | en_US |
dc.identifier.email | Corke, H: harold@hku.hk | en_US |
dc.identifier.authority | Corke, H=rp00688 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.carres.2009.05.032 | en_US |
dc.identifier.pmid | 19604502 | - |
dc.identifier.scopus | eid_2-s2.0-68949167939 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-68949167939&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 344 | en_US |
dc.identifier.issue | 13 | en_US |
dc.identifier.spage | 1701 | en_US |
dc.identifier.epage | 1708 | en_US |
dc.identifier.isi | WOS:000269991600016 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Kong, X=7202794616 | en_US |
dc.identifier.scopusauthorid | Corke, H=7007102942 | en_US |
dc.identifier.scopusauthorid | Bertoft, E=6604042426 | en_US |
dc.identifier.citeulike | 5203822 | - |
dc.identifier.issnl | 0008-6215 | - |