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- Publisher Website: 10.1007/s11356-012-0887-z
- Scopus: eid_2-s2.0-84871848370
- PMID: 22828878
- WOS: WOS:000306790200003
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Article: Gender-specific modulation of immune system complement gene expression in marine medaka Oryzias melastigma following dietary exposure of BDE-47.
Title | Gender-specific modulation of immune system complement gene expression in marine medaka Oryzias melastigma following dietary exposure of BDE-47. |
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Authors | |
Keywords | BDE-47 C3 Complement system Gender difference Immunomodulatory effects Marine medaka PBDEs |
Issue Date | 2011 |
Citation | Environmental Science And Pollution Research International, 2011, v. 19 n. 7, p. 2477-2487 How to Cite? |
Abstract | BDE-47 is one of the most widely found congeners of PBDEs in marine environments. The potential immunomodulatory effects of BDE-47 on fish complement system were studied using the marine medaka Oryzias melastigma as a model fish. Three-month-old O. melastigma were subjected to short-term (5 days) and long-term (21 days) exposure to two concentrations of BDE-47 (low dose at 290 ± 172 ng/day; high dose at 580 ± 344 ng/day) via dietary uptake of BDE-47 encapsulated in Artemia nauplii. Body burdens of BDE-47 and other metabolic products were analyzed in the exposed and control fish. Only a small amount of debrominated product, BDE-28, was detected, while other metabolic products were all under detection limit. Transcriptional expression of six major complement system genes involved in complement activation: C1r/s (classical pathway), MBL-2 (lectin pathway), CFP (alternative pathway), F2 (coagulation pathway), C3 (the central component of complement system), and C9 (cell lysis) were quantified in the liver of marine medaka. Endogenous expression of all six complement system genes was found to be higher in males than in females (p < 0.05). Upon dietary exposure of marine medaka to BDE-47, expression of all six complement genes were downregulated in males at day 5 (or longer), whereas in females, MBl-2, CFP, and F2 mRNAs expression were upregulated, but C3 and C9 remained stable with exposure time and dose. A significant negative relationship was found between BDE-47 body burden and mRNA expression of C1r/s, CFP, and C3 in male fish (r = -0.8576 to -0.9447). The above findings on changes in complement gene expression patterns indicate the complement system may be compromised in male O. melastigma upon dietary exposure to BDE-47. Distinct gender difference in expression of six major complement system genes was evident in marine medaka under resting condition and dietary BDE-47 challenge. The immunomodulatory effects of BDE-47 on transcriptional expression of these complement components in marine medaka were likely induced by the parent compound instead of biotransformed products. Our results clearly demonstrate that future direction for fish immunotoxicology and risk assessment of immunosuppressive chemicals must include parallel evaluation for both genders. |
Persistent Identifier | http://hdl.handle.net/10722/179323 |
ISSN | 2022 Impact Factor: 5.8 2023 SCImago Journal Rankings: 1.006 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Ye, RR | en_US |
dc.contributor.author | Lei, EN | en_US |
dc.contributor.author | Lam, MH | en_US |
dc.contributor.author | Chan, AK | en_US |
dc.contributor.author | Bo, J | en_US |
dc.contributor.author | Van De Merwe, JP | en_US |
dc.contributor.author | Fong, AC | en_US |
dc.contributor.author | Yang, MM | en_US |
dc.contributor.author | Lee, JS | en_US |
dc.contributor.author | Segner, HE | en_US |
dc.contributor.author | Wong, CK | en_US |
dc.contributor.author | Wu, RS | en_US |
dc.contributor.author | Au, DW | en_US |
dc.date.accessioned | 2012-12-19T09:54:10Z | - |
dc.date.available | 2012-12-19T09:54:10Z | - |
dc.date.issued | 2011 | en_US |
dc.identifier.citation | Environmental Science And Pollution Research International, 2011, v. 19 n. 7, p. 2477-2487 | en_US |
dc.identifier.issn | 1614-7499 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179323 | - |
dc.description.abstract | BDE-47 is one of the most widely found congeners of PBDEs in marine environments. The potential immunomodulatory effects of BDE-47 on fish complement system were studied using the marine medaka Oryzias melastigma as a model fish. Three-month-old O. melastigma were subjected to short-term (5 days) and long-term (21 days) exposure to two concentrations of BDE-47 (low dose at 290 ± 172 ng/day; high dose at 580 ± 344 ng/day) via dietary uptake of BDE-47 encapsulated in Artemia nauplii. Body burdens of BDE-47 and other metabolic products were analyzed in the exposed and control fish. Only a small amount of debrominated product, BDE-28, was detected, while other metabolic products were all under detection limit. Transcriptional expression of six major complement system genes involved in complement activation: C1r/s (classical pathway), MBL-2 (lectin pathway), CFP (alternative pathway), F2 (coagulation pathway), C3 (the central component of complement system), and C9 (cell lysis) were quantified in the liver of marine medaka. Endogenous expression of all six complement system genes was found to be higher in males than in females (p < 0.05). Upon dietary exposure of marine medaka to BDE-47, expression of all six complement genes were downregulated in males at day 5 (or longer), whereas in females, MBl-2, CFP, and F2 mRNAs expression were upregulated, but C3 and C9 remained stable with exposure time and dose. A significant negative relationship was found between BDE-47 body burden and mRNA expression of C1r/s, CFP, and C3 in male fish (r = -0.8576 to -0.9447). The above findings on changes in complement gene expression patterns indicate the complement system may be compromised in male O. melastigma upon dietary exposure to BDE-47. Distinct gender difference in expression of six major complement system genes was evident in marine medaka under resting condition and dietary BDE-47 challenge. The immunomodulatory effects of BDE-47 on transcriptional expression of these complement components in marine medaka were likely induced by the parent compound instead of biotransformed products. Our results clearly demonstrate that future direction for fish immunotoxicology and risk assessment of immunosuppressive chemicals must include parallel evaluation for both genders. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Environmental science and pollution research international | en_US |
dc.subject | BDE-47 | - |
dc.subject | C3 | - |
dc.subject | Complement system | - |
dc.subject | Gender difference | - |
dc.subject | Immunomodulatory effects | - |
dc.subject | Marine medaka | - |
dc.subject | PBDEs | - |
dc.subject.mesh | Administration, Oral | en_US |
dc.subject.mesh | Animal Feed | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Artemia | en_US |
dc.subject.mesh | Complement System Proteins - Genetics - Metabolism | en_US |
dc.subject.mesh | Diet | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Fish Proteins - Genetics - Metabolism | en_US |
dc.subject.mesh | Gene Expression Regulation - Immunology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Oryzias | en_US |
dc.subject.mesh | Polybrominated Biphenyls - Administration & Dosage - Toxicity | en_US |
dc.subject.mesh | Rna, Messenger - Genetics - Metabolism | en_US |
dc.subject.mesh | Sex Factors | en_US |
dc.subject.mesh | Water Pollutants, Chemical - Administration & Dosage - Toxicity | en_US |
dc.title | Gender-specific modulation of immune system complement gene expression in marine medaka Oryzias melastigma following dietary exposure of BDE-47. | en_US |
dc.type | Article | en_US |
dc.identifier.email | Wu, RS: rudolfwu@hku.hk | en_US |
dc.identifier.authority | Wu, RS=rp01398 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1007/s11356-012-0887-z | en_US |
dc.identifier.pmid | 22828878 | - |
dc.identifier.scopus | eid_2-s2.0-84871848370 | en_US |
dc.identifier.volume | 19 | en_US |
dc.identifier.issue | 7 | en_US |
dc.identifier.spage | 2477 | en_US |
dc.identifier.epage | 2487 | en_US |
dc.identifier.isi | WOS:000306790200003 | - |
dc.identifier.scopusauthorid | Ye, RR=55389042100 | en_US |
dc.identifier.scopusauthorid | Lei, EN=36465365000 | en_US |
dc.identifier.scopusauthorid | Lam, MH=7202630175 | en_US |
dc.identifier.scopusauthorid | Chan, AK=24278666900 | en_US |
dc.identifier.scopusauthorid | Bo, J=55389621300 | en_US |
dc.identifier.scopusauthorid | van de Merwe, JP=8701906400 | en_US |
dc.identifier.scopusauthorid | Fong, AC=55389444300 | en_US |
dc.identifier.scopusauthorid | Yang, MM=55389097600 | en_US |
dc.identifier.scopusauthorid | Lee, JS=55389852000 | en_US |
dc.identifier.scopusauthorid | Segner, HE=7004956984 | en_US |
dc.identifier.scopusauthorid | Wong, CK=35276549400 | en_US |
dc.identifier.scopusauthorid | Wu, RS=7402945079 | en_US |
dc.identifier.scopusauthorid | Au, DW=7004909228 | en_US |
dc.identifier.issnl | 0944-1344 | - |