Determination on the contents of condensed tannins in Spatholobus suberectus Dunn. extracts and primary study on their anti-tumor activities

Abstract

The contents of total flavonoids and condensed tannins in the ethanol extract of Spatholobus suberectus Dunn and its different solvents partitioned extracts were determined by ultraviolet spectrophotometry and Vanillin-H 2SO 4 method, respectively. The contents of total flavonoids in the φ=60% ethanol extract, ethyl acetate insoluble extract, ethyl acetate partitioned extract, n-butanol partitioned extract, water residue were (6.49±1.99)%, (7.36±0.13)%, (9.35±0.22)%, (8.41±0.27)%, (4.48±1.53)%, respectively, while their contents of condensed tannins were (51.09±0.37)%, (52.34±4.73)%, (73.22±2.72)%, (83.11±5.87)%, (29.74±2.12)%, respectively. The inhibitory activities on the proliferation of HT-29 and MCF-7 tumor cells were determined by MTT. The extract samples all showed inhibitory activities in different extent on the proliferation of HT-29 and MCF-7 tumor cells. The n-butanol partitioned extract had the strongest inhibitory activities on the proliferation of both tumor cells.