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Article: Antibody-mediated enhancement of Flavivirus replication in macrophage-like cell lines

TitleAntibody-mediated enhancement of Flavivirus replication in macrophage-like cell lines
Authors
Peiris, JSMPorterfield, JS
Issue Date1979
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/nature
Citation
Nature, 1979, v. 282 n. 5738, p. 509-511 How to Cite?
DOI: http://dx.doi.org/10.1038/282509a0
AbstractInteractions between animal viruses and antiviral antisera may exceptionally result in an apparent increase in viral infectivity. Halstead and coworkers demonstrated enhanced replication of dengue virus (a Flavivirus, family Togaviridae) in human or simian peripheral blood leucocytes carrying Fc receptors at subneutralising concentrations of antidengue antibody. We have used three continuous cell lines which express macrophage markers to explore the mechanism of this phenomenon. Dengue virus failed to replicate in these cells, but West Nile virus, another Flavivirus, replicated in all three, and we were able to demonstrate reproducibly 50-100-fold enhancement of virus yields in the presence of Flavivirus antisera, the effect also being directly demonstrable in P388D1 cells by increased numbers of virus-induced plaques. The phenomenon of antibody-dependent enhancement of viral replication is not unique to dengue virus, and may have far wider relevance in other viral infections.
Persistent Identifierhttp://hdl.handle.net/10722/179726
ISSN
0028-0836
2023 Impact Factor: 50.5
2023 SCImago Journal Rankings: 18.509
ISI Accession Number ID
WOS:A1979HV64300055

 

DC FieldValueLanguage
dc.contributor.authorPeiris, JSMen_US
dc.contributor.authorPorterfield, JSen_US
dc.date.accessioned2012-12-19T10:04:05Z-
dc.date.available2012-12-19T10:04:05Z-
dc.date.issued1979en_US
dc.identifier.citationNature, 1979, v. 282 n. 5738, p. 509-511en_US
dc.identifier.issn0028-0836en_US
dc.identifier.urihttp://hdl.handle.net/10722/179726-
dc.description.abstractInteractions between animal viruses and antiviral antisera may exceptionally result in an apparent increase in viral infectivity. Halstead and coworkers demonstrated enhanced replication of dengue virus (a Flavivirus, family Togaviridae) in human or simian peripheral blood leucocytes carrying Fc receptors at subneutralising concentrations of antidengue antibody. We have used three continuous cell lines which express macrophage markers to explore the mechanism of this phenomenon. Dengue virus failed to replicate in these cells, but West Nile virus, another Flavivirus, replicated in all three, and we were able to demonstrate reproducibly 50-100-fold enhancement of virus yields in the presence of Flavivirus antisera, the effect also being directly demonstrable in P388D1 cells by increased numbers of virus-induced plaques. The phenomenon of antibody-dependent enhancement of viral replication is not unique to dengue virus, and may have far wider relevance in other viral infections.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/natureen_US
dc.relation.ispartofNatureen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Viralen_US
dc.subject.meshAntigen-Antibody Reactionsen_US
dc.subject.meshArboviruses - Genetics - Immunologyen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCross Reactionsen_US
dc.subject.meshCytopathogenic Effect, Viralen_US
dc.subject.meshMacrophages - Immunology - Microbiologyen_US
dc.subject.meshMiceen_US
dc.subject.meshReceptors, Fc - Metabolismen_US
dc.subject.meshVirus Replicationen_US
dc.titleAntibody-mediated enhancement of Flavivirus replication in macrophage-like cell linesen_US
dc.typeArticleen_US
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_US
dc.identifier.authorityPeiris, JSM=rp00410en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/282509a0-
dc.identifier.pmid503230-
dc.identifier.scopuseid_2-s2.0-0018636837en_US
dc.identifier.volume282en_US
dc.identifier.issue5738en_US
dc.identifier.spage509en_US
dc.identifier.epage511en_US
dc.identifier.isiWOS:A1979HV64300055-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_US
dc.identifier.scopusauthoridPorterfield, JS=7005608715en_US
dc.identifier.issnl0028-0836-

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