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- Scopus: eid_2-s2.0-0025284774
- PMID: 1692862
- WOS: WOS:A1990DF84400043
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Article: Target antigens of transmission blocking immunity of Plasmodium vivax malaria. Characterization and polymorphism in natural parasite isolates
Title | Target antigens of transmission blocking immunity of Plasmodium vivax malaria. Characterization and polymorphism in natural parasite isolates |
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Authors | |
Issue Date | 1990 |
Publisher | American Association of Immunologists. The Journal's web site is located at http://www.jimmunol.org |
Citation | Journal Of Immunology, 1990, v. 144 n. 11, p. 4376-4383 How to Cite? |
Abstract | A panel of 20 anti-Plasmodium vivax female gamete mAb has been established and was characterized with respect to their transmission-blocking properties in membrane-feeding experiments and their target Ag identified. Seven mAb suppressed the infectivity of P. vivax parasites to Anopheles tesselatus mosquitoes. The m.w. of the Ag recognized by these mAb were ascertained by SDS-PAGE and Western blots. Three sets of polypeptides of low M(r) - 20, 24, and a doublet of 37/42 kDa - have been defined as traget Ag of transmission-blocking antibodies of P. vivax. All epitopes of these target Ag were found to be dependent on the tertiary conformational structure of the Ag. Polymorphism of target Ag of transmission-blocking immunity was investigated in over 30 natural isolates of P. vivax in Sri Lanka based on the reactivity of a mAb with an isolate as assessed by the indirect immunofluorescent test with the use of live extracellular female gametes, and in Western blots with the use of extracted gametes. The functional consequences of antigenic polymorphism on immunity was investigated in transmission-blocking assays by using membrane-feeding experiments. A majority of target Ag of transmission-blocking immunity were found to be polymorphic, exhibiting size as well as epitope polymorphism. Results indicate that failure of a mAb to affect the inefectivity of a parasite isolate of P. vivax to mosquitoes can be caused by polymorphism of the target Ag among isolates. |
Persistent Identifier | http://hdl.handle.net/10722/179740 |
ISSN | 2023 Impact Factor: 3.6 2023 SCImago Journal Rankings: 1.558 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Premawansa, S | en_US |
dc.contributor.author | Peiris, JSM | en_US |
dc.contributor.author | Perera Lakshman, KLR | en_US |
dc.contributor.author | Ariyaratne, G | en_US |
dc.contributor.author | Carter, R | en_US |
dc.contributor.author | Mendis, KN | en_US |
dc.date.accessioned | 2012-12-19T10:04:13Z | - |
dc.date.available | 2012-12-19T10:04:13Z | - |
dc.date.issued | 1990 | en_US |
dc.identifier.citation | Journal Of Immunology, 1990, v. 144 n. 11, p. 4376-4383 | en_US |
dc.identifier.issn | 0022-1767 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179740 | - |
dc.description.abstract | A panel of 20 anti-Plasmodium vivax female gamete mAb has been established and was characterized with respect to their transmission-blocking properties in membrane-feeding experiments and their target Ag identified. Seven mAb suppressed the infectivity of P. vivax parasites to Anopheles tesselatus mosquitoes. The m.w. of the Ag recognized by these mAb were ascertained by SDS-PAGE and Western blots. Three sets of polypeptides of low M(r) - 20, 24, and a doublet of 37/42 kDa - have been defined as traget Ag of transmission-blocking antibodies of P. vivax. All epitopes of these target Ag were found to be dependent on the tertiary conformational structure of the Ag. Polymorphism of target Ag of transmission-blocking immunity was investigated in over 30 natural isolates of P. vivax in Sri Lanka based on the reactivity of a mAb with an isolate as assessed by the indirect immunofluorescent test with the use of live extracellular female gametes, and in Western blots with the use of extracted gametes. The functional consequences of antigenic polymorphism on immunity was investigated in transmission-blocking assays by using membrane-feeding experiments. A majority of target Ag of transmission-blocking immunity were found to be polymorphic, exhibiting size as well as epitope polymorphism. Results indicate that failure of a mAb to affect the inefectivity of a parasite isolate of P. vivax to mosquitoes can be caused by polymorphism of the target Ag among isolates. | en_US |
dc.language | eng | en_US |
dc.publisher | American Association of Immunologists. The Journal's web site is located at http://www.jimmunol.org | en_US |
dc.relation.ispartof | Journal of Immunology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antibodies, Monoclonal - Immunology | en_US |
dc.subject.mesh | Antibodies, Protozoan - Genetics - Immunology | en_US |
dc.subject.mesh | Antigens, Protozoan - Genetics - Immunology | en_US |
dc.subject.mesh | Antigens, Surface - Immunology | en_US |
dc.subject.mesh | Blotting, Western | en_US |
dc.subject.mesh | Culicidae - Parasitology | en_US |
dc.subject.mesh | Epitopes | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Malaria - Immunology | en_US |
dc.subject.mesh | Molecular Weight | en_US |
dc.subject.mesh | Ovum - Immunology | en_US |
dc.subject.mesh | Plasmodium Vivax - Immunology - Pathogenicity | en_US |
dc.subject.mesh | Polymorphism, Genetic | en_US |
dc.title | Target antigens of transmission blocking immunity of Plasmodium vivax malaria. Characterization and polymorphism in natural parasite isolates | en_US |
dc.type | Article | en_US |
dc.identifier.email | Peiris, JSM: malik@hkucc.hku.hk | en_US |
dc.identifier.authority | Peiris, JSM=rp00410 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 1692862 | - |
dc.identifier.scopus | eid_2-s2.0-0025284774 | en_US |
dc.identifier.volume | 144 | en_US |
dc.identifier.issue | 11 | en_US |
dc.identifier.spage | 4376 | en_US |
dc.identifier.epage | 4383 | en_US |
dc.identifier.isi | WOS:A1990DF84400043 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Premawansa, S=6601964639 | en_US |
dc.identifier.scopusauthorid | Peiris, JSM=7005486823 | en_US |
dc.identifier.scopusauthorid | Perera Lakshman, KLR=6504713351 | en_US |
dc.identifier.scopusauthorid | Ariyaratne, G=7801423153 | en_US |
dc.identifier.scopusauthorid | Carter, R=7402936927 | en_US |
dc.identifier.scopusauthorid | Mendis, KN=7004958149 | en_US |
dc.identifier.issnl | 0022-1767 | - |