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- PMID: 11514393
- WOS: WOS:000170636600004
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Article: Effects of blood-processing protocols on fetal and total DNA quantification in maternal plasma
Title | Effects of blood-processing protocols on fetal and total DNA quantification in maternal plasma |
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Authors | |
Issue Date | 2001 |
Publisher | American Association for Clinical Chemistry, Inc. The Journal's web site is located at http://www.clinchem.org |
Citation | Clinical Chemistry, 2001, v. 47 n. 9, p. 1607-1613 How to Cite? |
Abstract | Background: Recently, apoptotic cells have been found in plasma obtained by centrifugation of blood from pregnant women, raising the question of what constitutes plasma and whether plasma is truly cell free. We compared the effects of different blood-processing protocols on the quantification, DNA composition, and day-to-day fluctuation of fetal and total DNA in maternal plasma. Methods: Blood samples were collected from healthy pregnant women. The blood sample from each individual was simultaneously processed by different means, including the following: Percoll separation, centrifugation, microcentrifugation, and filtration. The resulting plasma aliquots were subjected to real-time quantitative amplification of the β-globin (for total DNA) and SRY (for fetal DNA) genes. The differences in the β-globin and SRY DNA concentrations and the degree of variation between the various plasma aliquots were assessed statistically. Results: Different protocols of blood processing significantly affected the quantification and the day-to-day fluctuation of total (P <0.001), but not fetal (quantification, P = 0.336; fluctuation, P = 0.206), DNA in maternal plasma. The quantitative difference could be attributed to the fact that efficacies of different protocols for generating cell-free plasma vary. Processing blood samples by centrifugation followed by filtration or microcentrifugation is effective in producing cell-free plasma. Conclusions: Standardization in plasma-processing protocols is needed for maternal plasma DNA analysis, especially for quantification of total DNA in maternal plasma. Such preanalytic factors may also affect other applications of plasma DNA analysis. © 2001 American Association for Clinical Chemistry. |
Persistent Identifier | http://hdl.handle.net/10722/179769 |
ISSN | 2023 Impact Factor: 7.1 2023 SCImago Journal Rankings: 1.460 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Chiu, RWK | en_US |
dc.contributor.author | Poon, LLM | en_US |
dc.contributor.author | Lau, TK | en_US |
dc.contributor.author | Leung, TN | en_US |
dc.contributor.author | Wong, EMC | en_US |
dc.contributor.author | Lo, YMD | en_US |
dc.date.accessioned | 2012-12-19T10:04:27Z | - |
dc.date.available | 2012-12-19T10:04:27Z | - |
dc.date.issued | 2001 | en_US |
dc.identifier.citation | Clinical Chemistry, 2001, v. 47 n. 9, p. 1607-1613 | en_US |
dc.identifier.issn | 0009-9147 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179769 | - |
dc.description.abstract | Background: Recently, apoptotic cells have been found in plasma obtained by centrifugation of blood from pregnant women, raising the question of what constitutes plasma and whether plasma is truly cell free. We compared the effects of different blood-processing protocols on the quantification, DNA composition, and day-to-day fluctuation of fetal and total DNA in maternal plasma. Methods: Blood samples were collected from healthy pregnant women. The blood sample from each individual was simultaneously processed by different means, including the following: Percoll separation, centrifugation, microcentrifugation, and filtration. The resulting plasma aliquots were subjected to real-time quantitative amplification of the β-globin (for total DNA) and SRY (for fetal DNA) genes. The differences in the β-globin and SRY DNA concentrations and the degree of variation between the various plasma aliquots were assessed statistically. Results: Different protocols of blood processing significantly affected the quantification and the day-to-day fluctuation of total (P <0.001), but not fetal (quantification, P = 0.336; fluctuation, P = 0.206), DNA in maternal plasma. The quantitative difference could be attributed to the fact that efficacies of different protocols for generating cell-free plasma vary. Processing blood samples by centrifugation followed by filtration or microcentrifugation is effective in producing cell-free plasma. Conclusions: Standardization in plasma-processing protocols is needed for maternal plasma DNA analysis, especially for quantification of total DNA in maternal plasma. Such preanalytic factors may also affect other applications of plasma DNA analysis. © 2001 American Association for Clinical Chemistry. | en_US |
dc.language | eng | en_US |
dc.publisher | American Association for Clinical Chemistry, Inc. The Journal's web site is located at http://www.clinchem.org | en_US |
dc.relation.ispartof | Clinical Chemistry | en_US |
dc.subject.mesh | Blood Specimen Collection - Methods | en_US |
dc.subject.mesh | Dna - Blood - Isolation & Purification | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Fetal Blood - Chemistry | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Polymerase Chain Reaction | en_US |
dc.subject.mesh | Pregnancy - Blood | en_US |
dc.title | Effects of blood-processing protocols on fetal and total DNA quantification in maternal plasma | en_US |
dc.type | Article | en_US |
dc.identifier.email | Poon, LLM: llmpoon@hkucc.hku.hk | en_US |
dc.identifier.authority | Poon, LLM=rp00484 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 11514393 | - |
dc.identifier.scopus | eid_2-s2.0-0034872431 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0034872431&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 47 | en_US |
dc.identifier.issue | 9 | en_US |
dc.identifier.spage | 1607 | en_US |
dc.identifier.epage | 1613 | en_US |
dc.identifier.isi | WOS:000170636600004 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Chiu, RWK=7103038413 | en_US |
dc.identifier.scopusauthorid | Poon, LLM=7005441747 | en_US |
dc.identifier.scopusauthorid | Lau, TK=24491963900 | en_US |
dc.identifier.scopusauthorid | Leung, TN=7202110927 | en_US |
dc.identifier.scopusauthorid | Wong, EMC=13409091100 | en_US |
dc.identifier.scopusauthorid | Lo, YMD=7401935391 | en_US |
dc.identifier.issnl | 0009-9147 | - |