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- Publisher Website: 10.1016/j.ymben.2006.04.004
- Scopus: eid_2-s2.0-33748801503
- PMID: 16797196
- WOS: WOS:000240600800009
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Article: Engineering of the redox imbalance of Fusarium oxysporum enables anaerobic growth on xylose
Title | Engineering of the redox imbalance of Fusarium oxysporum enables anaerobic growth on xylose |
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Authors | |
Keywords | 13C-labelling experiments Fusarium oxysporum Metabolic network analysis Nitrogen source Redox balance |
Issue Date | 2006 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/meteng |
Citation | Metabolic Engineering, 2006, v. 8 n. 5, p. 474-482 How to Cite? |
Abstract | Dissimilatory nitrate reduction metabolism, of the natural xylose-fermenting fungus Fusarium oxysporum, was used as a strategy to achieve anaerobic growth and ethanol production from xylose. Beneficial alterations of the redox fluxes and thereby of the xylose metabolism were obtained by taking advantage of the regeneration of the cofactor NAD+ during the denitrification process. In batch cultivations, nitrate sustained growth under anaerobic conditions (1.21 g L-1 biomass) and simultaneously a maximum yield of 0.55 moles of ethanol per mole of xylose was achieved, whereas substitution of nitrate with ammonium limited the growth significantly (0.15 g L-1 biomass). Using nitrate, the maximum acetate yield was 0.21 moles per mole of xylose and no xylitol excretion was observed. Furthermore, the network structure in the central carbon metabolism of F. oxysporum was characterized in steady state. F. oxysporum grew anaerobically on [1-13C] labelled glucose and unlabelled xylose in chemostat cultivation with nitrate as nitrogen source. The use of labelled substrate allowed the precise determination of the glucose and xylose contribution to the carbon fluxes in the central metabolism of this poorly described microorganism. It was demonstrated that dissimilatory nitrate reduction allows F. oxysporum to exhibit typical respiratory metabolic behaviour with a highly active TCA cycle and a large demand for NADPH. © 2006 Elsevier Inc. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/181241 |
ISSN | 2023 Impact Factor: 6.8 2023 SCImago Journal Rankings: 1.853 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Panagiotou, G | en_US |
dc.contributor.author | Christakopoulos, P | en_US |
dc.contributor.author | Grotkjær, T | en_US |
dc.contributor.author | Olsson, L | en_US |
dc.date.accessioned | 2013-02-21T02:03:26Z | - |
dc.date.available | 2013-02-21T02:03:26Z | - |
dc.date.issued | 2006 | en_US |
dc.identifier.citation | Metabolic Engineering, 2006, v. 8 n. 5, p. 474-482 | en_US |
dc.identifier.issn | 1096-7176 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/181241 | - |
dc.description.abstract | Dissimilatory nitrate reduction metabolism, of the natural xylose-fermenting fungus Fusarium oxysporum, was used as a strategy to achieve anaerobic growth and ethanol production from xylose. Beneficial alterations of the redox fluxes and thereby of the xylose metabolism were obtained by taking advantage of the regeneration of the cofactor NAD+ during the denitrification process. In batch cultivations, nitrate sustained growth under anaerobic conditions (1.21 g L-1 biomass) and simultaneously a maximum yield of 0.55 moles of ethanol per mole of xylose was achieved, whereas substitution of nitrate with ammonium limited the growth significantly (0.15 g L-1 biomass). Using nitrate, the maximum acetate yield was 0.21 moles per mole of xylose and no xylitol excretion was observed. Furthermore, the network structure in the central carbon metabolism of F. oxysporum was characterized in steady state. F. oxysporum grew anaerobically on [1-13C] labelled glucose and unlabelled xylose in chemostat cultivation with nitrate as nitrogen source. The use of labelled substrate allowed the precise determination of the glucose and xylose contribution to the carbon fluxes in the central metabolism of this poorly described microorganism. It was demonstrated that dissimilatory nitrate reduction allows F. oxysporum to exhibit typical respiratory metabolic behaviour with a highly active TCA cycle and a large demand for NADPH. © 2006 Elsevier Inc. All rights reserved. | en_US |
dc.language | eng | en_US |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/meteng | en_US |
dc.relation.ispartof | Metabolic Engineering | en_US |
dc.subject | 13C-labelling experiments | - |
dc.subject | Fusarium oxysporum | - |
dc.subject | Metabolic network analysis | - |
dc.subject | Nitrogen source | - |
dc.subject | Redox balance | - |
dc.subject.mesh | Anaerobiosis - Physiology | en_US |
dc.subject.mesh | Bioreactors - Microbiology | en_US |
dc.subject.mesh | Cell Culture Techniques - Methods | en_US |
dc.subject.mesh | Cell Proliferation | en_US |
dc.subject.mesh | Fusarium - Physiology | en_US |
dc.subject.mesh | Genetic Enhancement - Methods | en_US |
dc.subject.mesh | Nitrates - Metabolism | en_US |
dc.subject.mesh | Oxidation-Reduction | en_US |
dc.subject.mesh | Xylose - Metabolism | en_US |
dc.title | Engineering of the redox imbalance of Fusarium oxysporum enables anaerobic growth on xylose | en_US |
dc.type | Article | en_US |
dc.identifier.email | Panagiotou, G: gipa@hku.hk | en_US |
dc.identifier.authority | Panagiotou, G=rp01725 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.ymben.2006.04.004 | en_US |
dc.identifier.pmid | 16797196 | - |
dc.identifier.scopus | eid_2-s2.0-33748801503 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-33748801503&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 8 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.spage | 474 | en_US |
dc.identifier.epage | 482 | en_US |
dc.identifier.isi | WOS:000240600800009 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Panagiotou, G=8566179700 | en_US |
dc.identifier.scopusauthorid | Christakopoulos, P=7006479823 | en_US |
dc.identifier.scopusauthorid | Grotkjær, T=6506918583 | en_US |
dc.identifier.scopusauthorid | Olsson, L=7203077540 | en_US |
dc.identifier.issnl | 1096-7176 | - |