File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1073/pnas.0709964105
- Scopus: eid_2-s2.0-41949120307
- PMID: 18332432
- WOS: WOS:000254263300057
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: A trispecies Aspergillus microarray: Comparative transcriptomics of three Aspergillus species
Title | A trispecies Aspergillus microarray: Comparative transcriptomics of three Aspergillus species |
---|---|
Authors | |
Keywords | Aspergillus Nidulans Aspergillus Niger Aspergillus Oryzae Xlnr |
Issue Date | 2008 |
Publisher | National Academy of Sciences. The Journal's web site is located at http://www.pnas.org |
Citation | Proceedings Of The National Academy Of Sciences Of The United States Of America, 2008, v. 105 n. 11, p. 4387-4392 How to Cite? |
Abstract | The full-genome sequencing of the filamentous fungi Aspergillus nidulans, Aspergillus niger, and Aspergillus oryzae has opened possibilities for studying the cellular physiology of these fungi on a systemic level. As a tool to explore this, we are making available an Affymetrix GeneChip developed for transcriptome analysis of any of the three above-mentioned aspergilli. Transcriptome analysis of triplicate batch cultivations of all three aspergilli on glucose and xylose media was used to validate the performance of the microarray. Gene comparisons of all three species and cross-analysis with the expression data identified 23 genes to be a conserved response across Aspergillus sp., including the xylose transcriptional activator XlnR. A promoter analysis of the upregulated genes in all three species indicates the conserved XlnR-binding site to be 5′-GGNTAAA-3′. The composition of the conserved gene-set suggests that xylose acts as a molecule, indicating the presence of complex carbohydrates such as hemicellulose, and triggers an array of degrading enzymes. With this case example, we present a validated tool for transcriptome analysis of three Aspergillus species and a methodology for conducting cross-species evolutionary studies within a genus using comparative transcriptomics. © 2008 by The National Academy of Sciences of the USA. |
Persistent Identifier | http://hdl.handle.net/10722/181249 |
ISSN | 2023 Impact Factor: 9.4 2023 SCImago Journal Rankings: 3.737 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Andersen, MR | en_US |
dc.contributor.author | Vongsangnak, W | en_US |
dc.contributor.author | Panagiotou, G | en_US |
dc.contributor.author | Salazar, MP | en_US |
dc.contributor.author | Lehmann, L | en_US |
dc.contributor.author | Nielsen, J | en_US |
dc.date.accessioned | 2013-02-21T02:03:29Z | - |
dc.date.available | 2013-02-21T02:03:29Z | - |
dc.date.issued | 2008 | en_US |
dc.identifier.citation | Proceedings Of The National Academy Of Sciences Of The United States Of America, 2008, v. 105 n. 11, p. 4387-4392 | en_US |
dc.identifier.issn | 0027-8424 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/181249 | - |
dc.description.abstract | The full-genome sequencing of the filamentous fungi Aspergillus nidulans, Aspergillus niger, and Aspergillus oryzae has opened possibilities for studying the cellular physiology of these fungi on a systemic level. As a tool to explore this, we are making available an Affymetrix GeneChip developed for transcriptome analysis of any of the three above-mentioned aspergilli. Transcriptome analysis of triplicate batch cultivations of all three aspergilli on glucose and xylose media was used to validate the performance of the microarray. Gene comparisons of all three species and cross-analysis with the expression data identified 23 genes to be a conserved response across Aspergillus sp., including the xylose transcriptional activator XlnR. A promoter analysis of the upregulated genes in all three species indicates the conserved XlnR-binding site to be 5′-GGNTAAA-3′. The composition of the conserved gene-set suggests that xylose acts as a molecule, indicating the presence of complex carbohydrates such as hemicellulose, and triggers an array of degrading enzymes. With this case example, we present a validated tool for transcriptome analysis of three Aspergillus species and a methodology for conducting cross-species evolutionary studies within a genus using comparative transcriptomics. © 2008 by The National Academy of Sciences of the USA. | en_US |
dc.language | eng | en_US |
dc.publisher | National Academy of Sciences. The Journal's web site is located at http://www.pnas.org | en_US |
dc.relation.ispartof | Proceedings of the National Academy of Sciences of the United States of America | en_US |
dc.subject | Aspergillus Nidulans | en_US |
dc.subject | Aspergillus Niger | en_US |
dc.subject | Aspergillus Oryzae | en_US |
dc.subject | Xlnr | en_US |
dc.title | A trispecies Aspergillus microarray: Comparative transcriptomics of three Aspergillus species | en_US |
dc.type | Article | en_US |
dc.identifier.email | Panagiotou, G: gipa@hku.hk | en_US |
dc.identifier.authority | Panagiotou, G=rp01725 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1073/pnas.0709964105 | en_US |
dc.identifier.pmid | 18332432 | - |
dc.identifier.scopus | eid_2-s2.0-41949120307 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-41949120307&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 105 | en_US |
dc.identifier.issue | 11 | en_US |
dc.identifier.spage | 4387 | en_US |
dc.identifier.epage | 4392 | en_US |
dc.identifier.isi | WOS:000254263300057 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Andersen, MR=15841796100 | en_US |
dc.identifier.scopusauthorid | Vongsangnak, W=24068171600 | en_US |
dc.identifier.scopusauthorid | Panagiotou, G=8566179700 | en_US |
dc.identifier.scopusauthorid | Salazar, MP=24067239400 | en_US |
dc.identifier.scopusauthorid | Lehmann, L=24066987200 | en_US |
dc.identifier.scopusauthorid | Nielsen, J=7404066338 | en_US |
dc.identifier.issnl | 0027-8424 | - |