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Article: Systems analysis unfolds the relationship between the phosphoketolase pathway and growth in Aspergillus nidulans
Title | Systems analysis unfolds the relationship between the phosphoketolase pathway and growth in Aspergillus nidulans |
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Authors | |
Issue Date | 2008 |
Publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action |
Citation | Plos One, 2008, v. 3 n. 12 How to Cite? |
Abstract | Background: Aspergillus nidulans is an important model organism for studies on fundamental eukaryotic cell biology and on industrial processes due to its close relation to A. niger and A. oryzae. Here we identified the gene coding for a novel metabolic pathway in A. nidulans, namely the phosphoketolase pathway, and investigated the role of an increased phosphoketolase activity. Methodology/Principal Findings: Over-expression of the phosphoketolase gene (phk) improved the specific growth rate on xylose, glycerol and ethanol. Transcriptome analysis showed that a total of 1,222 genes were significantly affected by overexpression of the phk, while more than half of the affected genes were carbon source specific. During growth on glucose medium, the transcriptome analysis showed that the response to phk over-expression is targeted to neutralize the effect of the over-expression by regulating the acetate metabolism and initiate a growth dampening response. Conclusions/Significance: Metabolic flux analysis using 13C-labelled glucose, showed that over-expression of phosphoketolase added flexibility to the central metabolism. Our findings further suggests that A. nidulans is not optimized for growth on xylose, glycerol or ethanol as the sole carbon sources. © 2008 Panagiotou et al. |
Persistent Identifier | http://hdl.handle.net/10722/181253 |
ISSN | 2023 Impact Factor: 2.9 2023 SCImago Journal Rankings: 0.839 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Panagiotou, G | en_US |
dc.contributor.author | Anderson, MR | en_US |
dc.contributor.author | Grotkjær, T | en_US |
dc.contributor.author | Regueira, TB | en_US |
dc.contributor.author | Hofmann, G | en_US |
dc.contributor.author | Nielsen, J | en_US |
dc.contributor.author | Olsson, L | en_US |
dc.date.accessioned | 2013-02-21T02:03:31Z | - |
dc.date.available | 2013-02-21T02:03:31Z | - |
dc.date.issued | 2008 | en_US |
dc.identifier.citation | Plos One, 2008, v. 3 n. 12 | en_US |
dc.identifier.issn | 1932-6203 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/181253 | - |
dc.description.abstract | Background: Aspergillus nidulans is an important model organism for studies on fundamental eukaryotic cell biology and on industrial processes due to its close relation to A. niger and A. oryzae. Here we identified the gene coding for a novel metabolic pathway in A. nidulans, namely the phosphoketolase pathway, and investigated the role of an increased phosphoketolase activity. Methodology/Principal Findings: Over-expression of the phosphoketolase gene (phk) improved the specific growth rate on xylose, glycerol and ethanol. Transcriptome analysis showed that a total of 1,222 genes were significantly affected by overexpression of the phk, while more than half of the affected genes were carbon source specific. During growth on glucose medium, the transcriptome analysis showed that the response to phk over-expression is targeted to neutralize the effect of the over-expression by regulating the acetate metabolism and initiate a growth dampening response. Conclusions/Significance: Metabolic flux analysis using 13C-labelled glucose, showed that over-expression of phosphoketolase added flexibility to the central metabolism. Our findings further suggests that A. nidulans is not optimized for growth on xylose, glycerol or ethanol as the sole carbon sources. © 2008 Panagiotou et al. | en_US |
dc.language | eng | en_US |
dc.publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | en_US |
dc.relation.ispartof | PLoS ONE | en_US |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Systems analysis unfolds the relationship between the phosphoketolase pathway and growth in Aspergillus nidulans | en_US |
dc.type | Article | en_US |
dc.identifier.email | Panagiotou, G: gipa@hku.hk | en_US |
dc.identifier.authority | Panagiotou, G=rp01725 | en_US |
dc.description.nature | published_or_final_version | en_US |
dc.identifier.doi | 10.1371/journal.pone.0003847 | en_US |
dc.identifier.pmid | 19052639 | - |
dc.identifier.scopus | eid_2-s2.0-57349128941 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-57349128941&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 3 | en_US |
dc.identifier.issue | 12 | en_US |
dc.identifier.isi | WOS:000265452200004 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Panagiotou, G=8566179700 | en_US |
dc.identifier.scopusauthorid | Anderson, MR=18133298200 | en_US |
dc.identifier.scopusauthorid | Grotkjær, T=6506918583 | en_US |
dc.identifier.scopusauthorid | Regueira, TB=25825375400 | en_US |
dc.identifier.scopusauthorid | Hofmann, G=7202274378 | en_US |
dc.identifier.scopusauthorid | Nielsen, J=7404066338 | en_US |
dc.identifier.scopusauthorid | Olsson, L=7203077540 | en_US |
dc.identifier.issnl | 1932-6203 | - |