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- Publisher Website: 10.1074/jbc.M111.337980
- Scopus: eid_2-s2.0-84860378380
- PMID: 22427655
- WOS: WOS:000304003200064
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Article: Leucine-rich repeat and WD repeat-containing protein 1 is recruited to pericentric heterochromatin by trimethylated lysine 9 of histone H3 and maintains heterochromatin silencing
Title | Leucine-rich repeat and WD repeat-containing protein 1 is recruited to pericentric heterochromatin by trimethylated lysine 9 of histone H3 and maintains heterochromatin silencing |
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Authors | |
Issue Date | 2012 |
Publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ |
Citation | Journal Of Biological Chemistry, 2012, v. 287 n. 18, p. 15024-15033 How to Cite? |
Abstract | Lrwd1, a protein containing a leucine-rich repeat and aWD40 repeat domain, interacts with the origin replication complex (ORC), a protein complex involved in both initiation of DNA replication and heterochromatin silencing. Lrwd1 and ORC are known to co-purify with repressive histone marks (trimethylated lysine 9 of histone H3 (H3K9me3) and trimethylated lysine 20 of histone H4 (H4K20me3)) and localize to pericentric heterochromatin. However, how the Lrwd1 is recruited to heterochromatin and the functional significance of the localization of Lrwd1 to the heterochromatin are not known. Here, we show that Lrwd1 preferentially binds to trimethylated repressive histone marks in vitro, which is dependent on an intact WD40 domain but independent of ORC proteins. The localization of Lrwd1 and Orc2 at pericentric heterochromatin in mouse cells is lost in cells lacking H3K9me3 but not in cells lacking H4K20me3. In addition, depletion of HP1α has little impact on the localization of Lrwd1 on pericentric heterochromatin. Finally, depletion of Lrwd1 and Orc2 in mouse cells leads to increased transcription of major satellite repeats. These results indicate that the Lrwd1 is recruited to pericentric heterochromatin through binding to H3K9me3 and that the association of Lrwd1 with pericentric heterochromatin is required for heterochromatin silencing and maintenance. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc. |
Persistent Identifier | http://hdl.handle.net/10722/184147 |
ISSN | 2020 Impact Factor: 5.157 2023 SCImago Journal Rankings: 1.766 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chan, KM | en_US |
dc.contributor.author | Zhang, Z | en_US |
dc.date.accessioned | 2013-06-25T03:00:21Z | - |
dc.date.available | 2013-06-25T03:00:21Z | - |
dc.date.issued | 2012 | en_US |
dc.identifier.citation | Journal Of Biological Chemistry, 2012, v. 287 n. 18, p. 15024-15033 | en_US |
dc.identifier.issn | 0021-9258 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/184147 | - |
dc.description.abstract | Lrwd1, a protein containing a leucine-rich repeat and aWD40 repeat domain, interacts with the origin replication complex (ORC), a protein complex involved in both initiation of DNA replication and heterochromatin silencing. Lrwd1 and ORC are known to co-purify with repressive histone marks (trimethylated lysine 9 of histone H3 (H3K9me3) and trimethylated lysine 20 of histone H4 (H4K20me3)) and localize to pericentric heterochromatin. However, how the Lrwd1 is recruited to heterochromatin and the functional significance of the localization of Lrwd1 to the heterochromatin are not known. Here, we show that Lrwd1 preferentially binds to trimethylated repressive histone marks in vitro, which is dependent on an intact WD40 domain but independent of ORC proteins. The localization of Lrwd1 and Orc2 at pericentric heterochromatin in mouse cells is lost in cells lacking H3K9me3 but not in cells lacking H4K20me3. In addition, depletion of HP1α has little impact on the localization of Lrwd1 on pericentric heterochromatin. Finally, depletion of Lrwd1 and Orc2 in mouse cells leads to increased transcription of major satellite repeats. These results indicate that the Lrwd1 is recruited to pericentric heterochromatin through binding to H3K9me3 and that the association of Lrwd1 with pericentric heterochromatin is required for heterochromatin silencing and maintenance. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc. | en_US |
dc.language | eng | en_US |
dc.publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ | en_US |
dc.relation.ispartof | Journal of Biological Chemistry | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Cell Line | en_US |
dc.subject.mesh | Chromosomal Proteins, Non-Histone - Genetics - Metabolism | en_US |
dc.subject.mesh | Gene Silencing - Physiology | en_US |
dc.subject.mesh | Heterochromatin - Genetics - Metabolism | en_US |
dc.subject.mesh | Histones - Genetics - Metabolism | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Mice, Knockout | en_US |
dc.subject.mesh | Microtubule Proteins - Genetics - Metabolism | en_US |
dc.subject.mesh | Origin Recognition Complex - Genetics - Metabolism | en_US |
dc.subject.mesh | Protein Binding - Physiology | en_US |
dc.subject.mesh | Transcription, Genetic - Physiology | en_US |
dc.title | Leucine-rich repeat and WD repeat-containing protein 1 is recruited to pericentric heterochromatin by trimethylated lysine 9 of histone H3 and maintains heterochromatin silencing | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chan, KM: ming616@graduate.hku.hk | en_US |
dc.identifier.authority | Chan, KM=rp01757 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1074/jbc.M111.337980 | en_US |
dc.identifier.pmid | 22427655 | - |
dc.identifier.scopus | eid_2-s2.0-84860378380 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-84860378380&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 287 | en_US |
dc.identifier.issue | 18 | en_US |
dc.identifier.spage | 15024 | en_US |
dc.identifier.epage | 15033 | en_US |
dc.identifier.isi | WOS:000304003200064 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Chan, KM=8631854500 | en_US |
dc.identifier.scopusauthorid | Zhang, Z=35239002800 | en_US |
dc.identifier.issnl | 0021-9258 | - |