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- Publisher Website: 10.1016/S0010-7824(84)80028-1
- Scopus: eid_2-s2.0-0021744186
- PMID: 6548951
- WOS: WOS:A1984TT57200007
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Article: Effect of reserpine on fertilizing capacity of human spermatozoa
Title | Effect of reserpine on fertilizing capacity of human spermatozoa |
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Authors | |
Issue Date | 1984 |
Publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/contraception |
Citation | Contraception, 1984, v. 30 n. 4, p. 363-369 How to Cite? |
Abstract | The effect of reserpine at various concentrations (2 x 10-6, 2 x 10-7, 2 x 10-8, 2 x 10-9 and 2 x 10-10M) on the in vitro fertilizing capacity of human spermatozoa was studied. Spermatozoa collected from presumably fertile men were washed in BWW medium and incubated with different concentrations of reserpine for 5 hr before insemination of the zona-free hamster ova. The spermatozoa penetration of the zona-free hamster ova was scored 6 hr later and the results were analyzed statistically. Reserpine, at all the concentrations tested, caused a significant dose-dependent decrease in the penetration of the denuded hamster ova in comparison to the control (p < 0.05). The percent mobility of spermatozoa decreased as a function of time during the preincubation period to initiate spermatozoal capacitation but there were no significant differences in the values between the control and the reserpine-treated spermatozoa (p > 0.05). These findings indicate that reserpine can affect the fertilizing capacity of human spermatozoa in vitro and provide an additional evidence to suggest the prospective use of reserpine as a vaginal contraceptive. | The effect of reserpine at various concentrations (2x10-6, 2x10-7 2x10-8, 2x10-9, and 2x10-10 m) on the in vitro fertilizing capacity of human spermatozoa was studied. Spermatozoa was collected from presumably fertile men and washed in BWW medium and incubated with different concentrations of reserpine for 5 hours prior to insemination of the zona-free hamster ova. The spermatozoal penetration of the zona-free hamster ova was scored 6 hours later and results were analyzed statistically. Reserpine, at all concentrations tested, caused a significant dose-dependent decrease in the penetration of the denuded hamster ova in comparison to the control (P0.05). The percent motility of spermatozoa decreased as a function of time during the preincubation period to initiate spermatozoal capacitation but there were no significant differences in the values between the control and the reserpine-treated sprmatozoa (P0.05). These findings indicate that reserpine can affect the fertilizing capacity of human spermatozoa in vitro and provide additional evidence to suggest the prospective use of reserpine as a vaginal contraceptive. author's modified |
Persistent Identifier | http://hdl.handle.net/10722/184163 |
ISSN | 2023 Impact Factor: 2.8 2023 SCImago Journal Rankings: 1.210 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chan, SYW | en_US |
dc.contributor.author | Tang, LCH | en_US |
dc.date.accessioned | 2013-06-25T03:00:54Z | - |
dc.date.available | 2013-06-25T03:00:54Z | - |
dc.date.issued | 1984 | en_US |
dc.identifier.citation | Contraception, 1984, v. 30 n. 4, p. 363-369 | en_US |
dc.identifier.issn | 0010-7824 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/184163 | - |
dc.description.abstract | The effect of reserpine at various concentrations (2 x 10-6, 2 x 10-7, 2 x 10-8, 2 x 10-9 and 2 x 10-10M) on the in vitro fertilizing capacity of human spermatozoa was studied. Spermatozoa collected from presumably fertile men were washed in BWW medium and incubated with different concentrations of reserpine for 5 hr before insemination of the zona-free hamster ova. The spermatozoa penetration of the zona-free hamster ova was scored 6 hr later and the results were analyzed statistically. Reserpine, at all the concentrations tested, caused a significant dose-dependent decrease in the penetration of the denuded hamster ova in comparison to the control (p < 0.05). The percent mobility of spermatozoa decreased as a function of time during the preincubation period to initiate spermatozoal capacitation but there were no significant differences in the values between the control and the reserpine-treated spermatozoa (p > 0.05). These findings indicate that reserpine can affect the fertilizing capacity of human spermatozoa in vitro and provide an additional evidence to suggest the prospective use of reserpine as a vaginal contraceptive. | The effect of reserpine at various concentrations (2x10-6, 2x10-7 2x10-8, 2x10-9, and 2x10-10 m) on the in vitro fertilizing capacity of human spermatozoa was studied. Spermatozoa was collected from presumably fertile men and washed in BWW medium and incubated with different concentrations of reserpine for 5 hours prior to insemination of the zona-free hamster ova. The spermatozoal penetration of the zona-free hamster ova was scored 6 hours later and results were analyzed statistically. Reserpine, at all concentrations tested, caused a significant dose-dependent decrease in the penetration of the denuded hamster ova in comparison to the control (P0.05). The percent motility of spermatozoa decreased as a function of time during the preincubation period to initiate spermatozoal capacitation but there were no significant differences in the values between the control and the reserpine-treated sprmatozoa (P0.05). These findings indicate that reserpine can affect the fertilizing capacity of human spermatozoa in vitro and provide additional evidence to suggest the prospective use of reserpine as a vaginal contraceptive. author's modified | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/contraception | en_US |
dc.relation.ispartof | Contraception | en_US |
dc.subject.mesh | Adult | en_US |
dc.subject.mesh | Dose-Response Relationship, Drug | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Fertilization - Drug Effects | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Reserpine - Pharmacology | en_US |
dc.subject.mesh | Sperm-Ovum Interactions - Drug Effects | en_US |
dc.subject.mesh | Spermatozoa - Drug Effects | en_US |
dc.title | Effect of reserpine on fertilizing capacity of human spermatozoa | en_US |
dc.type | Article | en_US |
dc.identifier.email | Tang, LCH: lchtang@hku.hk | en_US |
dc.identifier.authority | Tang, LCH=rp01756 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/S0010-7824(84)80028-1 | - |
dc.identifier.pmid | 6548951 | - |
dc.identifier.scopus | eid_2-s2.0-0021744186 | en_US |
dc.identifier.volume | 30 | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.spage | 363 | en_US |
dc.identifier.epage | 369 | en_US |
dc.identifier.isi | WOS:A1984TT57200007 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Chan, SYW=7404255960 | en_US |
dc.identifier.scopusauthorid | Tang, LCH=7402081111 | en_US |
dc.identifier.issnl | 0010-7824 | - |