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- Publisher Website: 10.1007/s00011-012-0584-x
- Scopus: eid_2-s2.0-84877135473
- PMID: 23266598
- WOS: WOS:000314776000008
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Article: Polysaccharides of Dendrobium officinale inhibit TNF-α-induced apoptosis in A-253 cell line
Title | Polysaccharides of Dendrobium officinale inhibit TNF-α-induced apoptosis in A-253 cell line |
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Authors | |
Keywords | A-253 Apoptosis Dendrobium officinale Polysaccharides TNF-α |
Issue Date | 2013 |
Citation | Inflammation Research, 2013, v. 62 n. 3, p. 313-324 How to Cite? |
Abstract | Objective: Our previous study demonstrated that polysaccharides of Dendrobium officinale Kimura et Migo (DP) were capable of enhancing immunomodulation in an experimental model of Sjögren's syndrome, a chronic autoimmune disease mainly affecting the salivary glands. In the present study, we further investigated the protective effect of DP on a human salivary gland cell line A-253 against tumor necrosis factor (TNF)-α-induced apoptosis. Materials: TNF-α (100 U/ml) was used as the stimulus for treating the A-253 cells to induce cellular apoptosis. Nuclear factor-kappa B (NF-κB, p65), phosphorylation of mitogen-activated protein kinases (MAPK), reactive oxygen species (ROS) generation, mitochondrial membrane potential and proapoptotic proteins were examined. A-253 cells were pre-treated with DP for 12 h before TNF-α stimulation. Results: We observed translocation of NF-κB into the nuclei, prolonged MAPK, excessive ROS generation and strongly decreased mitochondrial membrane potential, and subsequently cytochrome C release and caspase-3 activation. However, pre-treatment with DP significantly inhibited the TNF-α-induced apoptotic factors. Conclusions: Our data suggested the inhibitory effect of DP on TNF-α-induced apoptosis in a human salivary gland cell line. This inhibition indicated potential inference of DP in the initial plasma membrane-bound complex of TNF-α and its receptors. © 2012 Springer Basel. |
Persistent Identifier | http://hdl.handle.net/10722/186043 |
ISSN | 2023 Impact Factor: 4.8 2023 SCImago Journal Rankings: 1.309 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Lin, X | en_US |
dc.contributor.author | Sze, CW | en_US |
dc.contributor.author | Ng, TB | en_US |
dc.contributor.author | Tong, Y | en_US |
dc.contributor.author | Shaw, PC | en_US |
dc.contributor.author | Tang, SCW | en_US |
dc.contributor.author | Zhang, Y | en_US |
dc.date.accessioned | 2013-08-20T11:50:17Z | - |
dc.date.available | 2013-08-20T11:50:17Z | - |
dc.date.issued | 2013 | en_US |
dc.identifier.citation | Inflammation Research, 2013, v. 62 n. 3, p. 313-324 | en_US |
dc.identifier.issn | 1023-3830 | - |
dc.identifier.uri | http://hdl.handle.net/10722/186043 | - |
dc.description.abstract | Objective: Our previous study demonstrated that polysaccharides of Dendrobium officinale Kimura et Migo (DP) were capable of enhancing immunomodulation in an experimental model of Sjögren's syndrome, a chronic autoimmune disease mainly affecting the salivary glands. In the present study, we further investigated the protective effect of DP on a human salivary gland cell line A-253 against tumor necrosis factor (TNF)-α-induced apoptosis. Materials: TNF-α (100 U/ml) was used as the stimulus for treating the A-253 cells to induce cellular apoptosis. Nuclear factor-kappa B (NF-κB, p65), phosphorylation of mitogen-activated protein kinases (MAPK), reactive oxygen species (ROS) generation, mitochondrial membrane potential and proapoptotic proteins were examined. A-253 cells were pre-treated with DP for 12 h before TNF-α stimulation. Results: We observed translocation of NF-κB into the nuclei, prolonged MAPK, excessive ROS generation and strongly decreased mitochondrial membrane potential, and subsequently cytochrome C release and caspase-3 activation. However, pre-treatment with DP significantly inhibited the TNF-α-induced apoptotic factors. Conclusions: Our data suggested the inhibitory effect of DP on TNF-α-induced apoptosis in a human salivary gland cell line. This inhibition indicated potential inference of DP in the initial plasma membrane-bound complex of TNF-α and its receptors. © 2012 Springer Basel. | - |
dc.language | eng | en_US |
dc.relation.ispartof | Inflammation Research | en_US |
dc.subject | A-253 | - |
dc.subject | Apoptosis | - |
dc.subject | Dendrobium officinale | - |
dc.subject | Polysaccharides | - |
dc.subject | TNF-α | - |
dc.title | Polysaccharides of Dendrobium officinale inhibit TNF-α-induced apoptosis in A-253 cell line | en_US |
dc.type | Article | en_US |
dc.identifier.email | Sze, CW: stephens@hku.hk | en_US |
dc.identifier.email | Tong, Y: tongyao@hku.hk | en_US |
dc.identifier.email | Tang, SCW: scwtang@hku.hk | en_US |
dc.identifier.email | Zhang, Y: ybzhang@hku.hk | en_US |
dc.identifier.authority | Sze, CW=rp00514 | en_US |
dc.identifier.authority | Tong, Y=rp00509 | en_US |
dc.identifier.authority | Tang, SCW=rp00480 | en_US |
dc.identifier.authority | Zhang, Y=rp01410 | en_US |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/s00011-012-0584-x | - |
dc.identifier.pmid | 23266598 | - |
dc.identifier.scopus | eid_2-s2.0-84877135473 | - |
dc.identifier.hkuros | 219451 | en_US |
dc.identifier.volume | 62 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 313 | en_US |
dc.identifier.epage | 324 | en_US |
dc.identifier.eissn | 1420-908X | - |
dc.identifier.isi | WOS:000314776000008 | - |
dc.identifier.issnl | 1023-3830 | - |