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Article: Tumor-derived autophagosomes (DRibbles) induce B cell activation in a TLR2-MyD88 dependent manner
Title | Tumor-derived autophagosomes (DRibbles) induce B cell activation in a TLR2-MyD88 dependent manner |
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Authors | |
Issue Date | 2013 |
Publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action |
Citation | PLoS One, 2013, v. 8 n. 1, p. e53564 How to Cite? |
Abstract | Previously, we have documented that isolated autophagosomes from tumor cells could efficiently cross-prime tumor-reactive naïve T cells and mediate tumor regression in preclinical mouse models. However, the effect of tumor-derived autophagosomes, here we refer as to DRibbles, on B cells has not been studied so far. At present study, we found that DRibbles generated from a murine hepatoma cell line Hep1-6, induced B-cell activation after intravenous injection into mice. B-cell populations were significantly expanded and the production of Hep1-6 tumor-specific antibodies was successfully induced. Moreover, in vitro studies showed that DRibbles could induce more efficient B-cell proliferation and activation, antibody production, and cytokine secretion than whole tumor cell lysates. Notably, we found that B-cell activation required proteins but not DNA in the DRibbles. We further showed that B cells could capture DRibbles and present antigens in the DRibbles to directly induce T cell activation. Furthermore, we found that B-cell activation, antibody production, cytokine secretion and antigen cross-presentation were TLR2-MyD88 pathway dependent. Taken together, the present studies demonstrated that tumor-derived autophagosomes (DRibbles) efficiently induced B cells activation, antibody production, cytokine secretion and antigen cross-presentation mainly depending on their protein component via TLR2/MyD88 dependent manner. |
Persistent Identifier | http://hdl.handle.net/10722/186442 |
ISSN | 2021 Impact Factor: 3.752 2020 SCImago Journal Rankings: 0.990 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Li, W | - |
dc.contributor.author | Zhou, M | - |
dc.contributor.author | Ren, H | - |
dc.contributor.author | Hu, HM | - |
dc.contributor.author | Lu, L | - |
dc.contributor.author | Cao, M | - |
dc.contributor.author | Wang, LX | - |
dc.date.accessioned | 2013-08-20T12:09:01Z | - |
dc.date.available | 2013-08-20T12:09:01Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | PLoS One, 2013, v. 8 n. 1, p. e53564 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/10722/186442 | - |
dc.description.abstract | Previously, we have documented that isolated autophagosomes from tumor cells could efficiently cross-prime tumor-reactive naïve T cells and mediate tumor regression in preclinical mouse models. However, the effect of tumor-derived autophagosomes, here we refer as to DRibbles, on B cells has not been studied so far. At present study, we found that DRibbles generated from a murine hepatoma cell line Hep1-6, induced B-cell activation after intravenous injection into mice. B-cell populations were significantly expanded and the production of Hep1-6 tumor-specific antibodies was successfully induced. Moreover, in vitro studies showed that DRibbles could induce more efficient B-cell proliferation and activation, antibody production, and cytokine secretion than whole tumor cell lysates. Notably, we found that B-cell activation required proteins but not DNA in the DRibbles. We further showed that B cells could capture DRibbles and present antigens in the DRibbles to directly induce T cell activation. Furthermore, we found that B-cell activation, antibody production, cytokine secretion and antigen cross-presentation were TLR2-MyD88 pathway dependent. Taken together, the present studies demonstrated that tumor-derived autophagosomes (DRibbles) efficiently induced B cells activation, antibody production, cytokine secretion and antigen cross-presentation mainly depending on their protein component via TLR2/MyD88 dependent manner. | - |
dc.language | eng | - |
dc.publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | - |
dc.relation.ispartof | PLoS ONE | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Tumor-derived autophagosomes (DRibbles) induce B cell activation in a TLR2-MyD88 dependent manner | - |
dc.type | Article | - |
dc.identifier.email | Lu, L: liweilu@hkucc.hku.hk | - |
dc.identifier.authority | Lu, L=rp00477 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1371/journal.pone.0053564 | - |
dc.identifier.pmcid | PMC3541185 | - |
dc.identifier.scopus | eid_2-s2.0-84872256620 | - |
dc.identifier.hkuros | 218878 | - |
dc.identifier.volume | 8 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | e53564 | - |
dc.identifier.epage | e53564 | - |
dc.identifier.isi | WOS:000313551500084 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1932-6203 | - |